A Substrate‐Independent Approach for the Surface Immobilization of Oligonucleotides using Aldehyde Functionalized Surfaces

Abstract

A highly efficient approach for the covalent immobilization of single-strand DNA onto solid surfaces and their subsequent hybridization to complementary strands is described. This entails pulsed plasma deposition of poly(3-vinylbenzaldehyde) followed by the Schiff-base reaction. A range of substrates, including glass slides, silicon wafers, polystyrene microspheres, and single-walled carbon nanotubes (SWNTs), are functionalized employing this methodology.