Revised classification of the DLA loci by serological studies

Krumbacher, K.; Feltz, M. J. M. van der; Happel, M.; Gerlach, Carmen; Lößlein, Lena; Grosse‐Wilde, H.

doi:10.1111/j.1399-0039.1986.tb01531.x

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Cited by 12 publications

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Immunological characterization of canine hematopoietic progenitor cells

Hahn¹,

Kolb²,

Schumm³

et al. 1991

Ann Hematol

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Canine hematopoietic progenitor cells were characterized by separation with monoclonal antibodies. Depleted and enriched fractions were studied for growth of CFU-GM in semisolid agar and for repopulating capacity of lethally irradiated dogs. CFU growth was not reduced by depletion of marrow using monoclonal antibodies F 3-20-7 (anti-dog Thy-1), MT606 (anti-human CD 6), and IOT2a (anti-human DR). CFU growth was variable following treatment with the anti-canine T-cell antibody MdT-P 1 and immunomagnetic bead separation. It was regularly enriched when MdT-P 1 treatment was followed by immunorosetting with staphylococcal protein A-loaded sheep red blood cells and density gradient separation. Lethally irradiated dogs were reconstituted by autologous marrow depleted of MdT-P 1-positive cells using immunorosetting and density gradient centrifugation, whereas immunomagnetic bead-depleted marrow was ineffective. Fluorescence-activated cell sorting showed enrichment of hematopoietic progenitor cells in the weakly MdT-P 1-positive fraction.

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