Nucleotide sequence of a dog class I cDNA clone

Sarmiento, Ulla M.; Storb, Rainer

doi:10.1007/bf02115019

Cited by 25 publications

(6 citation statements)

References 19 publications

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“…The predicted amino acid sequences of clones 8-9 and 1-29 (excluding the leader peptide) share from 80.9% to 86.6% sequence similarity with the protein sequences of cotton-top tamarin (Watkins et al 1990), human (Zemmour and Parham 1993), dog (Sarmiento and Storb 1990), and cattle (Ennis et al 1988 Me CA r g I I e TM 1 Met P r o P roThr P h e Leu L eu L eu L eu S e rG I yA! a L eu g e r Le u Th r G i u qh r LeUAI a G l y S e r Hi S S e r Me U A r g Ty • P h e TY r Th rGlyVa l S e rat _o P r oG i y Ar g G l y G i u P roAr .…”

Section: Horse Edna Clones Encoding Two Mhc Class I Genesmentioning

confidence: 99%

Horse cDNA clones encoding two MHC class I genes

et al. 1994

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Section: Horse Edna Clones Encoding Two Mhc Class I Genesmentioning

confidence: 99%

Horse cDNA clones encoding two MHC class I genes

et al. 1994

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“…The first of these to be identified was DLA-79, which was originally isolated from a canine phage library following screening with a human HLA-E cDNA probe (12). In separate experiments, a single clone, 116, was isolated from a canine cDNA library using a human HLA-B7 probe (13). I16 was found to be 80% identical with the HLA-A, -B and -C consensus sequence and was subsequently used to screen three canine genomic libraries.…”

mentioning

confidence: 99%

Polymorphism analysis of four canine MHC class I genes

et al. 1998

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We have studied the variability of four structurally complete dog leukocyte antigen @LA) class I genes, termed DLA-12, -88, -79 and -64, in a population of mixed breed, unrelated dogs. The human HLA and canine DLA loci share a high degree of similarity in terms of gene structure. This analysis focused on the first three exons of each of four complete canine genes. Exons two and three are the major source of polymorphism in the corresponding human genes. In this analysis, DLA-88 was found to be significantly more polymorphic than the other three genes, with 44 distinct alleles observed among 63 mixed breed, unrelated dogs. The remaining

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“…The different approaches used by these methods, distance vs. discrete characters, respectively, allow focussing on branch patterns that are supported by statistical anlyses with both methods. (Parham et al 1989); gorilla (Ggo) (Lawlor et al 1991); orangatuan (Ppy) (Lawlor et al 1990); cottontop tamarin (Soe) (Watkins et al 1991); horse (Eta) ( Barbis et al 1994); sheep (Oar) (Grossberger, Hein, and Marcuz 1990); cow (Bta) (Brown, Spooner, and Clark 1989); rabbit (Ocu) (Tykocinski et al 1984); dog (Cfa) (Sarmiento and Storb 1990); mouse (Mmu) H-2!? (Hasenkrug and Nathenson 1991), H-2Kf (Horton et al 1990), Kk (Arnold et al 1984), Kd (Kvist, Roberts, and Dobberstein 1983), Dk (Watts et al 1987), Db (Watts et al 1987), QIOk (Watts et al 1989), and M3 (Wang, Loveland, and Lindahl 1991); rat (Rno) RT1.A" (Rada et al 1990) and RT1.Ab (Radojcic et al 1989); hamster (Mau) (McGuire, Duncan, and Tucker 1986); and red-necked wallaby (Mru) (Mayer et al 1993).…”

Section: Resultsmentioning

confidence: 99%

“…The lack of indels in human and mouse class I genes suggests that they are rare in the second and third exons of class I sequences from these species. However, indels have been identified in class I sequences from four other mammals including dogs, cotton-top tamatins, Rhesus macaques, and red-necked wallabies (Sarmiento and Storb 1990;Watkins et al 1991;Otting and Bontrop 1993;Mayer et al 1993). The insertions in the latter three species share similarities with the Scab insertion in that they occurred on l3 strands or adjacent loops in pocket B.…”

Section: Fig 7-alignmentmentioning

confidence: 99%

The divergence of major histocompatibility complex class I genes in Sciurus aberti

Wettstein¹,

Strausbauch²,

Johnston³

et al. 1996

Molecular Biology and Evolution

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Major histocompatibility complex (MHC) class I genes encode cell surface molecules that bind and present immunogenic peptides to cytolytic T lymphocytes. We have developed a model using the MHC (Scab) of tassel-eared squirrels, Sciurus aberti, to investigate the evolution of MHC genes in subspecies that have been effectively isolated in comparable, if not identical, environments. Class I cDNAs from single S. aberti aberti and S. aberti kaibabensis squirrels were cloned and sequenced. These two cDNA transcripts differed by nucleotide substitutions that were predominantly located in codons impacting the peptide binding site, and nonsynonymous substitutions exceeded synonymous substitutions at these sites. These sequences also differed by the insertion of two amino acids in a beta-strand adjacent to position 45 in pocket B of the peptide binding site that may result in a deeper pocket with altered peptide specificity. This indel is present in additional Scab class I sequences, and class I sequences in five subspecies carry identically sized insertions. Phylogenetic analyses of exons 2, 3, and 4 with neighbor-joining and maximum parsimony methods depict that Scab class I sequences diverged at a point intermediate between murid class I sequences and class I sequences of primates, carnivores, and artiodactyls. The relative relatedness of Scab class I sequences to those in the latter group appears to be founded in relative similarities in exons 2 and 3, which encode the peptide binding site. These results bring into question the use of a single model for rodent class I sequences. Moreover, they demonstrate that the inclusion of exons 2 and 3 in phylogenetic analyses of class I may obscure true phylogenetic relationships, perhaps due to convergence through strong selective pressure.

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Nucleotide sequence of a dog class I cDNA clone

Cited by 25 publications

References 19 publications

Horse cDNA clones encoding two MHC class I genes

Horse cDNA clones encoding two MHC class I genes

Polymorphism analysis of four canine MHC class I genes

The divergence of major histocompatibility complex class I genes in Sciurus aberti

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