Revised annotation and extended characterizations of components of the <i>Chlamydomonas reinhardtii</i> SUMOylation system

Lin, Yen‐Ling; Chung, Chin-Lin; Huang, Pin jui; Chen, Chun‐Han; Fang, Su‐Chiung

doi:10.1002/pld3.266

Cited by 5 publications

(3 citation statements)

References 127 publications

(235 reference statements)

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“…The proteomic profiles and subsequent MS/MS revealed a DeSI-like protein, a putative protease enzyme [ 52 , 53 , 54 ]. DeSI-like protein showed an increasing accumulation over the different infective stages (T1–T3; Figure 4 ), suggesting that the protein catabolism occurs during the invasive process.…”

Section: Resultsmentioning

confidence: 99%

“…DNA replication licensing factor MCM2 [ 77 ] and WEB family proteins [ 78 , 79 ] are synchronized and prepare the cell cycle progression, respectively, while leucine-rich repeat-containing protein DDB is involved in chromatin and DNA dynamics [ 79 ]. On the other hand, DeSI-like (peptidase-like) protein is a protein involved in nitrogen catabolism [ 52 , 80 , 81 ], and these nitrogen derivates could be translocated and transformed into nitrogen reserves in seeds or be responsible for the emergence of seedlings [ 82 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, in P. calyculatus , that infects mesquite trees, there are no reports indicating that it secretes lignocellulolytic and peptidase enzymes. In that sense, we detected a group of proteins that were accumulated in the main infective stages such as pectinesterase 15 and DeSI-like (peptidase-like) protein that are likely cell wall-degrading and peptidase enzymes, respectively [ 52 , 53 , 54 , 80 , 81 , 116 ]. A pectin methylesterase enzyme was secreted from calli and seedlings of Orobranche aegyptiaca and O. cumana and it was speculated that the enzyme may have a function in host infection [ 51 , 117 , 118 ].…”

Section: Discussionmentioning

confidence: 99%

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Protein Profiling of Psittacanthus calyculatus during Mesquite Infection

Aguilar-Venegas

Quintana-Rodríguez²,

Aguilar-Hernández³

et al. 2023

Plants

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Psittacanthus calyculatus is a hemiparasite mistletoe that represents an ecological problem due to the impacts caused to various tree species of ecological and commercial interest. Although the life cycle for the Psittacanthus genus is well established in the literature, the development stages and molecular mechanism implicated in P. calyculatus host infection are poorly understood. In this study, we used a manageable infestation of P. laevigata with P. calyculatus to clearly trace the infection, which allowed us to describe five phenological infective stages of mistletoe on host tree branches: mature seed (T1), holdfast formation (T2), haustorium activation (T3), haustorium penetration (T4), and haustorium connection (T5) with the host tree. Proteomic analyses revealed proteins with a different accumulation and cellular processes in infective stages. Activities of the cell wall-degrading enzymes cellulase and β-1,4-glucosidase were primarily active in haustorium development (T3), while xylanase, endo-glucanase, and peptidase were highly active in the haustorium penetration and xylem connection (T4). Patterns of auxins and cytokinin showed spatial concentrations in infective stages and moreover were involved in haustorium development. These results are the first evidence of proteins, cell wall-degrading enzymes, and phytohormones that are involved in early infection for the Psittacanthus genus, and thus represent a general infection mechanism for other mistletoe species. These results could help to understand the molecular dialogue in the establishment of P. calyculatus parasitism.

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Section: Resultsmentioning

confidence: 99%