Review—Single-Molecule Sensors Based on Protein Nanopores

Zuo, Jiaqi; Song, Ningning; Wang, Jia; Zhao, Xian; Cheng, Man; Wang, Qinyi; Tang, Wen; Yang, Zekai; Qiu, Kaipei

doi:10.1149/1945-7111/ac39da

Cited by 7 publications

(4 citation statements)

References 201 publications

(341 reference statements)

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“…Therefore, their detection is achieved through a specific blockade fingerprint, correlated with ensuing alterations in the net ionic current across it. The subsequent on- or off-line statistical analysis of the resulting current fluctuations, generates information regarding the analyte’s identity and its physical and chemical properties [ 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ].…”

Section: Introductionmentioning

confidence: 99%

Probing the Hepatitis B Virus E-Antigen with a Nanopore Sensor Based on Collisional Events Analysis

et al. 2022

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Real-time monitoring, simple operation, and cheaper methods for detecting immunological proteins hold the potential for a solid influence on proteomics and human biology, as they can promote the onset of timely diagnoses and adequate treatment protocols. In this work we present an exploratory study suggesting the applicability of resistive-pulse sensing technology in conjunction with the α-hemolysin (α-HL) protein nanopore, for the detection of the chronic hepatitis B virus (HBV) e-antigen (HBeAg). In this approach, the recognition between HBeAg and a purified monoclonal hepatitis B e antibody (Ab(HBeAg)) was detected via transient ionic current spikes generated by partial occlusions of the α-HL nanopore by protein aggregates electrophoretically driven toward the nanopore’s vestibule entrance. Despite the steric hindrance precluding antigen, antibody, or antigen–antibody complex capture inside the nanopore, their stochastic bumping with the nanopore generated clear transient blockade events. The subsequent analysis suggested the detection of protein subpopulations in solution, rendering the approach a potentially valuable label-free platform for the sensitive, submicromolar-scale screening of HBeAg targets.

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Section: Introductionmentioning

confidence: 99%

Probing the Hepatitis B Virus E-Antigen with a Nanopore Sensor Based on Collisional Events Analysis

et al. 2022

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“…When a voltage is applied across the nanopore, a major part of the electrical field is confined within a small radius around the nanopore (typically within a few microns), usually referred to as the capture radius [423,424]. Therefore, nanopores mostly capture the analytes available within the very tiny capture volume, which in turn limits the nanopore's performance [14,[425][426][427]. To overcome this limitation, Rahman et al have demonstrated an elegant solution developed on the previously described ARROW optofluidic platform that can improve the performance of nanopore sensing by increasing the detection rate of analytes [422].…”

Section: Electrical Methods For Single-molecule Experimentsmentioning

confidence: 99%

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

Rahman

Islam

et al. 2022

Micromachines

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Single-molecule techniques have shifted the paradigm of biological measurements from ensemble measurements to probing individual molecules and propelled a rapid revolution in related fields. Compared to ensemble measurements of biomolecules, single-molecule techniques provide a breadth of information with a high spatial and temporal resolution at the molecular level. Usually, optical and electrical methods are two commonly employed methods for probing single molecules, and some platforms even offer the integration of these two methods such as optofluidics. The recent spark in technological advancement and the tremendous leap in fabrication techniques, microfluidics, and integrated optofluidics are paving the way toward low cost, chip-scale, portable, and point-of-care diagnostic and single-molecule analysis tools. This review provides the fundamentals and overview of commonly employed single-molecule methods including optical methods, electrical methods, force-based methods, combinatorial integrated methods, etc. In most single-molecule experiments, the ability to manipulate and exercise precise control over individual molecules plays a vital role, which sometimes defines the capabilities and limits of the operation. This review discusses different manipulation techniques including sorting and trapping individual particles. An insight into the control of single molecules is provided that mainly discusses the recent development of electrical control over single molecules. Overall, this review is designed to provide the fundamentals and recent advancements in different single-molecule techniques and their applications, with a special focus on the detection, manipulation, and control of single molecules on chip-scale devices.

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“…Biological nanopores can be produced from a series of materials, including pore-forming proteins, peptides, and DNA by insertion into lipid membranes, liposomes, or polymer membranes. 30,42,43 Apart from the aHL nanopore, other protein nanopores including aerolysin, 44,45 mycobacterium smegmatis porin A (MspA), [46][47][48] cytotoxic cytolysin A (ClyA) [49][50][51] and outer membrane proteins F (OmpF) 52,53 and G (OmpG), 54 have also been discovered (Fig. 3).…”

Section: Categories Of Nanoporesmentioning

confidence: 99%