Review Neutrophil Gelatinase-Associated Lipocalin (NGAL): Perannya sebagai Biomarker pada Kerusakan Ginjal Akut

Syadiah, Ami Rahmati; Febrina, Ellin; Levita, Jutti

doi:10.25077/jsfk.8.1.35-42.2021

Cited by 1 publication

(1 citation statement)

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“…Nephrotoxicity is indicated by changes in glomerular filtration rate (normal GFR in young adults is 120 mL/minute), proximal tubular cell toxicity, inflammation, etc., and the enzymes present in tubular epithelial cells leak into the urine and can be determined as nephrotoxic biomarkers [ 1 ]. Such biomarkers, e.g., neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), and calprotectin, are used as an early diagnosis of acute and chronic kidney injury [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

The Cytotoxicity and Nephroprotective Activity of the Ethanol Extracts of Angelica keiskei Koidzumi Stems and Leaves against the NAPQI-Induced Human Embryonic Kidney (HEK293) Cell Line

Amalia

Aulifa

Zain

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Ethnopharmacological Relevance. In Indonesia, Angelica keiskei Koidzumi (ashitaba or Japanese celery) has been traditionally used to maintain health and to achieve longevity. Previously, the chlorophyll-rich extract of A. keiskei planted in Korea exhibited a strong antioxidant activity. The objective of the present study was to investigate the cytotoxicity and nephroprotective activity of the ethanol extract of A. keiskei Koidzumi on the N-acetyl-p-benzoquinone imine (NAPQI) induced human embryonic kidney (HEK293) cell line. Materials and Methods. A. keiskei Koidzumi plant was collected from Mount Rinjani, Lombok, Indonesia, and was identified at the School of Biology Sciences and Technology, Bandung Institute of Technology, Indonesia. Extraction of the stems (ASE) and leaves (ALE) was performed by employing ethanol 70% for 3 × 24 h at 26°C. The cytotoxicity study of the extracts was assessed using the water-soluble tetrazolium salt-8 (WST-8) reagent on the HEK293 cell line, while the nephroprotective activity assay was determined on the NAPQI-induced HEK293 cell line. Results. The WST-8 assay showed that the cytotoxicity IC50 of ASE = 2322 μg/mL and IC50 of ALE = 2283 μg/mL. The nephroprotective activity assay revealed that ASE possesses nephroprotective activity against the NAPQI-induced HEK293 cell line at 1161 μg/mL, while ALE does not show the nephroprotective activity. Conclusion. Taken together, lower concentrations of ASE and ALE (<2000 μg/mL) are not toxic to the HEK293 cell line, and only ASE indicates the activity to protect the HEK293 cell line against NAPQI damage. This Japanese celery could be further explored for its potential as a plant-based nephroprotective drug.

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Section: Introductionmentioning

confidence: 99%