Review: doxorubicin delivery systems based on chitosan for cancer therapy

Tan, Mei Lin; Choong, Peter; Dass, Crispin R.

doi:10.1211/jpp/61.02.0001

Cited by 51 publications

(36 citation statements)

References 95 publications

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“…Previous works by Jain and colleagues 46 have demonstrated that the increases in tumour vascular permeability form the basis for nanoparticle extravasation and drug delivery. Recently, works by Dass and colleagues 48 have shown that chitosan-based delivery systems are efficient for cancer therapy. Along with these studies, current advances in nanotechnology have produced synthetic nanoparticles to encapsulate and release a pre-programmed diversity of therapeutic measures against cancers 1,2,4,49-51 .…”

Section: Discussionmentioning

confidence: 99%

Delivery of chemotherapeutic drugs in tumour cell-derived microparticles

et al. 2012

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Cellular microparticles are vesicular plasma membrane fragments with a diameter of 100-1,000 nanometres that are shed by cells in response to various physiological and artificial stimuli. Here we demonstrate that tumour cell-derived microparticles can be used as vectors to deliver chemotherapeutic drugs. We show that tumour cells incubated with chemotherapeutic drugs package these drugs into microparticles, which can be collected and used to effectively kill tumour cells in murine tumour models without typical side effects. We describe several mechanisms involved in this process, including uptake of drug-containing microparticles by tumour cells, synthesis of additional drug-packaging microparticles by these cells that contribute to the cytotoxic effect and the inhibition of drug efflux from tumour cells. This study highlights a novel drug delivery strategy with potential clinical application.

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Section: Discussionmentioning

confidence: 99%

Delivery of chemotherapeutic drugs in tumour cell-derived microparticles

et al. 2012

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“…[16][17][18] The second class includes doxorubicin and daunorubicin; they inhibit class II topoisomerases progression, preventing the DNA double helix from being resealed and therefore stopping cell division. 19,20 Two cell lines derive from lung carcinoma (A549 and A427); two other cell lines come from prostate carcinoma (PC-3 and DU145). The last three (T98G, U373 and Hs683) are glioblastoma.…”

Section: Introductionmentioning

confidence: 99%

The effect of anticancer drugs on seven cell lines monitored by FTIR spectroscopy

Derenne

Verdonck

Goormaghtigh

2012

Analyst

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Systemic approaches such as metabolomics are increasingly needed to improve the development of novel drugs. In this paper, we suggest a new strategy based on infrared spectroscopy which probes the global chemical composition of a sample. Seven cell lines from three tumour types were investigated and exposed to four classical anticancer drugs belonging to two classes characterized by a unique mechanism. First, each cell line was considered separately and a hierarchical clustering was built for the seven cell lines. Spectra clustered according to the drug mechanism of action for all the cell lines tested. Second, the similarities among drug mechanism spectral fingerprints were investigated for all the cell lines simultaneously. Difference spectra (the mean spectrum of the corresponding untreated cell line was subtracted) were computed so that the particular contribution of every cell line was eliminated and only the drug-induced differences could be compared. The hierarchical clustering shows a clear tendency to distinguish the two modes of action, revealing a very similar type of response to molecules with a similar mechanism. High throughput systems with 96-well plates are now available and a well established bioassay could be developed in order to provide an objective classifier for potential anticancer drugs.

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“…[18][19][20] Chitosan is a carbohydrate polymer with the desirable properties of biodegradability and biocompatibility that have made it a candidate polymer for preparation of drug delivery carriers. [21][22][23][24][25][26][27][28][29] Several methods have been developed for the preparation of doxorubicin delivery systems based on chitosan, 30 which include dextran sulfate-chitosan hydrogel nanoparticles, glycol-chitosan nanoaggregates, oleoyl-chitosan nanoparticles, chitosan-poly(acrylic acid) hollow nanospheres, and stearic acid-grafted chitosan oligosaccharide micelles.…”

Section: Introductionmentioning

confidence: 99%

Targeted delivery of doxorubicin-utilizing chitosan nanoparticles surface-functionalized with anti-Her2 trastuzumab

Sakhtianchi¹,

Atyabi²,

Yousef³

et al. 2011

IJN

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Background Targeting drugs to their sites of action to overcome the systemic side effects associated with most antineoplastic agents is still a major challenge in pharmaceutical research. In this study, the monoclonal antibody, trastuzumab, was used as a targeting agent in nanoparticles carrying the antitumor drug, doxorubicin, specifically to its site of action. Methods Chitosan-doxorubicin conjugation was carried out using succinic anhydride as a crosslinker. Trastuzumab was conjugated to self-assembled chitosan-doxorubin conjugate (CS-DOX) nanoparticles (particle size, 200 nm) via thiolation of lysine residues and subsequent linking of the resulted thiols to chitosan. Conjugation was confirmed by gel permeation chromatography, differential scanning calorimetry, Fourier transform infrared spectroscopy, and 1 H nuclear magnetic resonance spectroscopy studies. Dynamic light scattering, transmission electron microscopy, and zeta potential determination were used to characterize the nanoparticles. Results CS-DOX conjugated nanoparticles had a spherical shape and smooth surface with a narrow size distribution and core-shell structure. Increasing the ratio of doxorubicin to chitosan in the conjugation reaction gave rise to a higher doxorubicin content but lower conjugation efficiency. Trastuzumab-decorated nanoparticles (CS-DOX-mAb) contained 47 μg/mg doxorubicin and 33.5 μg/mg trastuzumab. Binding of trastuzumab to the nanoparticles was further probed thermodynamically by isothermal titration calorimetry. Fluorescence microscopy demonstrated enhanced and selective uptake of CS-DOX-mAb by Her2 + cancer cells compared with nontargeted CS-DOX nanoparticles and free drug. Conclusion Antibody-conjugated nanoparticles were shown to discriminate between Her2 + and Her2 − cells, and thus have the potential to be used in active targeted drug delivery, with reduction of drug side effects in Her2 + breast and ovarian cancers.

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Review: doxorubicin delivery systems based on chitosan for cancer therapy

Cited by 51 publications

References 95 publications

Delivery of chemotherapeutic drugs in tumour cell-derived microparticles

Delivery of chemotherapeutic drugs in tumour cell-derived microparticles

The effect of anticancer drugs on seven cell lines monitored by FTIR spectroscopy

Targeted delivery of doxorubicin-utilizing chitosan nanoparticles surface-functionalized with anti-Her2 trastuzumab

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