Review criteria for assessment of antimicrobial susceptibility devices—what do they mean?

Tomfohrde, K. M.

doi:10.1016/0196-4399(91)90070-c

Cited by 3 publications

(3 citation statements)

References 2 publications

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“…Major errors were observed for CNS-oxacillin and S. agalactiaetetracycline combinations. In conclusion the results of this study indicate that the VITEK 2 system represents an accurate and acceptable means for performing identification and antibiotic susceptibility tests with medically relevant gram-positive cocci.Automation in clinical microbiology is still in a very early stage of development compared with the level of automation that has been achieved in clinical chemistry, hematology, and immunology laboratories.In the last 20 years, a variety of automated systems for the identification and antimicrobial susceptibility testing (AST) of microorganisms has been developed based on automated interpretation of the results of biochemical tests or using microdilution trays following overnight incubation and photometric determination of growth (6,18,20,21). Advances in technology that may provide rapid bacterial identification and AST are now recognized as having both clinical and financial benefits (2).…”

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“…In the last 20 years, a variety of automated systems for the identification and antimicrobial susceptibility testing (AST) of microorganisms has been developed based on automated interpretation of the results of biochemical tests or using microdilution trays following overnight incubation and photometric determination of growth (6,18,20,21). Advances in technology that may provide rapid bacterial identification and AST are now recognized as having both clinical and financial benefits (2).…”

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Evaluation of the VITEK 2 System for Identification and Antimicrobial Susceptibility Testing of Medically Relevant Gram-Positive Cocci

et al. 2002

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A study was conducted to evaluate the new VITEK 2 system (bioMérieux) for identification and antibiotic susceptibility testing of gram-positive cocci. Clinical isolates of Staphylococcus aureus (n ‫؍‬ 100), coagulasenegative staphylococci (CNS) (n ‫؍‬ 100), Enterococcus spp. (n ‫؍‬ 89), Streptococcus agalactiae (n ‫؍‬ 29), and Streptococcus pneumoniae (n ‫؍‬ 66) were examined with the ID-GPC identification card and with the AST-P515 (for staphylococci), AST-P516 (for enterococci and S. agalactiae) and AST-P506 (for pneumococci) susceptibility cards. The identification comparison methods were the API Staph for staphylococci and the API 20 Strep for streptococci and enterococci; for antimicrobial susceptibility testing, the agar dilution method according to the procedure of the National Committee for Clinical Laboratory Standards (NCCLS) was used. The VITEK 2 system correctly identified to the species level (only one choice or after simple supplementary tests) 99% of S. aureus, 96.5% of S. agalactiae, 96.9% of S. pneumoniae, 92.7% of Enterococcus faecalis, 91.3% of Staphylococcus haemolyticus, and 88% of Staphylococcus epidermidis but was least able to identify Enterococcus faecium (71.4% correct). More than 90% of gram-positive cocci were identified within 3 h. According to the NCCLS breakpoints, antimicrobial susceptibility testing with the VITEK 2 system gave 96% correct category agreement, 0.82% very major errors, 0.17% major errors, and 2.7% minor errors. Antimicrobial susceptibility testing showed category agreement from 94 to 100% for S. aureus, from 90 to 100% for CNS, from 91 to 100% for enterococci, from 96 to 100% for S. agalactiae, and from 91 to 100% for S. pneumoniae. Microorganism-antibiotic combinations that gave very major errors were CNS-erythromycin, CNS-oxacillin, enterococci-teicoplanin, and enterococci-high-concentration gentamicin. Major errors were observed for CNS-oxacillin and S. agalactiaetetracycline combinations. In conclusion the results of this study indicate that the VITEK 2 system represents an accurate and acceptable means for performing identification and antibiotic susceptibility tests with medically relevant gram-positive cocci.Automation in clinical microbiology is still in a very early stage of development compared with the level of automation that has been achieved in clinical chemistry, hematology, and immunology laboratories.In the last 20 years, a variety of automated systems for the identification and antimicrobial susceptibility testing (AST) of microorganisms has been developed based on automated interpretation of the results of biochemical tests or using microdilution trays following overnight incubation and photometric determination of growth (6,18,20,21). Advances in technology that may provide rapid bacterial identification and AST are now recognized as having both clinical and financial benefits (2).The VITEK system originated in the 1970s as an automated system for identification and AST and has evolved today into the VITEK 2 system, which automatically performs ...

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Evaluation of the VITEK 2 System for Identification and Antimicrobial Susceptibility Testing of Medically Relevant Gram-Positive Cocci

et al. 2002

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“…The other criteria proposed by Metzler and Dehaan (11) (false susceptibility of Ͻ1% and false resistance of Ͻ5% for all tests), by Jorgensen (10) (false susceptibility plus false resistance of Ͻ7%), and by Thornsberry and Gavan (18) (complete category agreement of Ͼ90% and false susceptibility plus false resistance of Ͻ5%) are met by using the ATB ANA device. The Food and Drug Admin- istration (FDA) has established minimal performance characteristics to assess antimicrobial susceptibility devices (19). These guidelines indicate that category agreement (applied to devices using category result formats) should be Ͼ90%, major errors should be Ͻ3% for susceptible strains, and very major errors should be Յ1.5% for resistant strains.…”

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Susceptibility Testing of Anaerobic Bacteria: Evaluation of the Redesigned (Version 96) bioMérieux ATB ANA Device

Dubreuil¹,

Houcke²,

Singer³

1999

J Clin Microbiol

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We compared the susceptibility results for 200 clinical anaerobes with nine antibiotics obtained by using a new ATB ANA (bioMérieux) device against those obtained by the National Committee for Clinical Laboratory Standards (NCCLS) standard agar dilution method. For better evaluation of the device, we added some resistant Bacteroides fragilis group strains from our own collection: 3, 6, and 12 strains that were resistant to imipenem, ticarcillin plus clavulanic acid, and co-amoxiclav, respectively, and 2 other strains with decreased susceptibility to metronidazole. For some strains that did not grow on ATB S medium, tests were performed by using West-Wilkins medium supplemented with 1.5% agar. The new ATB ANA device made clinical categorization of the investigated strains possible, according to French (Committee of the Antibiogram of the French Society of Microbiology) or U.S. (NCCLS) breakpoints, with the following respective results: category agreement, 94.3 and 94.9%; minor errors, 4.8 and 3.8%; major errors, 0.4 and 0.8%; and very major errors 4.6 and 4.2%. The ATB ANA device was able to detect low-level metronidazole-resistant B. fragilis strains according to the French breakpoints but not the NCCLS ones. For B. fragilis and β-lactamase-positive Prevotellastrains, the clustering effect of amoxicillin MICs around the French breakpoints led to more frequent minor errors. ATB ANA is a very convenient method to determine the antibiotic susceptibilities of anaerobes. Results obtained by ATB ANA correlated well with those obtained by the reference method.

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Evaluation of the Etest for susceptibility testing of anaerobic bacteria

Rosenblatt

Gustafson

1995

Diagnostic Microbiology and Infectious Disease

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Review criteria for assessment of antimicrobial susceptibility devices—what do they mean?

Cited by 3 publications

References 2 publications

Evaluation of the VITEK 2 System for Identification and Antimicrobial Susceptibility Testing of Medically Relevant Gram-Positive Cocci

Evaluation of the VITEK 2 System for Identification and Antimicrobial Susceptibility Testing of Medically Relevant Gram-Positive Cocci

Susceptibility Testing of Anaerobic Bacteria: Evaluation of the Redesigned (Version 96) bioMérieux ATB ANA Device

Evaluation of the Etest for susceptibility testing of anaerobic bacteria

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