Abstract:Due to the annual increase in CO2 emissions, climate change continues to progress. In order to achieve the goals set in the Paris Climate Agreement, CO2 capturing measures are necessary in addition to the reduction in carbon dioxide emissions. This review article describes and discusses the CO2 capturing methods published in the last 20 years. Processes for absorption, gas–solid reaction, adsorption, cryogenic processes, membrane processes and the capture of carbon dioxide with natural inclusion were examined … Show more
“…Further, the expression level of induced HepG2 cells in aggregates was significantly higher (≈1.7×) than in 2D culture. Taken together, these results confirmed that the HepG2 cell aggregates provided a beneficial culturing condition as previously reported for hepatocytes such as HepaRG [ 68–70 ] or primary human hepatocytes [ 66,71 ] when grown in spheroids. However, the present method of aggregate formation by rendering surfaces non‐adhesive with alginate, rather than producing single spheroids in, for example, ultra‐low attachment wells, presents an easy means of scaling up the production of aggregates.…”
Section: Resultssupporting
confidence: 89%
“…CYP1A2 is one of the dominant enzymes of the CYP450 family that are involved in the phase I metabolism of the liver, [ 65 ] and is therefore an essential feature in the current effort. HepG2 cells are well known to have a limited inherent CYP1A2 activity compared to primary hepatocytes, [ 66 ] but the expression can be induced by treatment with the AhR agonist β‐napthoflavone (BNF). [ 67 ] The monitored production of resorufin by the HepG2 cells was normalized to the number of cells by quantifying the double‐stranded DNA (dsDNA, Figure S4, Supporting Information).…”
Section: Resultsmentioning
confidence: 99%
“…Consequently, the aim was to add activity to AC 0 that boosted a liver‐relevant function in the printed semi‐synthetic tissue. We chose to implement a cytochrome P450‐related activity due to its importance in liver performance, and the documented limited function thereof in HepG2 cells, [ 66 ] thereby potentially partly mitigating a shortcoming of this widely used cell line. Thus, the incorporation of CYP450 activity was chosen as a proof‐of‐concept to illustrate that artificial cells can be designed to boost a specific hepatic function.…”
Artificial cells are engineered units with cell‐like functions for different purposes including acting as supportive elements for mammalian cells. Artificial cells with minimal liver‐like function are made of alginate and equipped with metalloporphyrins that mimic the enzyme activity of a member of the cytochrome P450 family namely CYP1A2. The artificial cells are employed to enhance the dealkylation activity within 3D bioprinted structures composed of HepG2 cells and these artificial cells. This enhancement is monitored through the conversion of resorufin ethyl ether to resorufin. HepG2 cell aggregates are 3D bioprinted using an alginate/gelatin methacryloyl ink, resulting in successful proliferation of the HepG2 cells. The composite ink made of an alginate/gelatin liquid phase with increasing amount of artificial cells is characterized. The CYP1A2‐like activity of artificial cells is preserved over at least 35 days, where 6 nM resorufin was produced in 8 hours. Composite inks made of artificial cells and HepG2 cell aggregates in a liquid phase are used for 3D bioprinting. The HepG2 cells proliferate over 35 days, and the structure has boosted CYP1A2 activity. The integration of artificial cells and their living counter parts into larger 3D semi‐synthetic tissues is a step towards exploring bottom‐up synthetic biology in tissue engineering.This article is protected by copyright. All rights reserved
“…Further, the expression level of induced HepG2 cells in aggregates was significantly higher (≈1.7×) than in 2D culture. Taken together, these results confirmed that the HepG2 cell aggregates provided a beneficial culturing condition as previously reported for hepatocytes such as HepaRG [ 68–70 ] or primary human hepatocytes [ 66,71 ] when grown in spheroids. However, the present method of aggregate formation by rendering surfaces non‐adhesive with alginate, rather than producing single spheroids in, for example, ultra‐low attachment wells, presents an easy means of scaling up the production of aggregates.…”
Section: Resultssupporting
confidence: 89%
“…CYP1A2 is one of the dominant enzymes of the CYP450 family that are involved in the phase I metabolism of the liver, [ 65 ] and is therefore an essential feature in the current effort. HepG2 cells are well known to have a limited inherent CYP1A2 activity compared to primary hepatocytes, [ 66 ] but the expression can be induced by treatment with the AhR agonist β‐napthoflavone (BNF). [ 67 ] The monitored production of resorufin by the HepG2 cells was normalized to the number of cells by quantifying the double‐stranded DNA (dsDNA, Figure S4, Supporting Information).…”
Section: Resultsmentioning
confidence: 99%
“…Consequently, the aim was to add activity to AC 0 that boosted a liver‐relevant function in the printed semi‐synthetic tissue. We chose to implement a cytochrome P450‐related activity due to its importance in liver performance, and the documented limited function thereof in HepG2 cells, [ 66 ] thereby potentially partly mitigating a shortcoming of this widely used cell line. Thus, the incorporation of CYP450 activity was chosen as a proof‐of‐concept to illustrate that artificial cells can be designed to boost a specific hepatic function.…”
Artificial cells are engineered units with cell‐like functions for different purposes including acting as supportive elements for mammalian cells. Artificial cells with minimal liver‐like function are made of alginate and equipped with metalloporphyrins that mimic the enzyme activity of a member of the cytochrome P450 family namely CYP1A2. The artificial cells are employed to enhance the dealkylation activity within 3D bioprinted structures composed of HepG2 cells and these artificial cells. This enhancement is monitored through the conversion of resorufin ethyl ether to resorufin. HepG2 cell aggregates are 3D bioprinted using an alginate/gelatin methacryloyl ink, resulting in successful proliferation of the HepG2 cells. The composite ink made of an alginate/gelatin liquid phase with increasing amount of artificial cells is characterized. The CYP1A2‐like activity of artificial cells is preserved over at least 35 days, where 6 nM resorufin was produced in 8 hours. Composite inks made of artificial cells and HepG2 cell aggregates in a liquid phase are used for 3D bioprinting. The HepG2 cells proliferate over 35 days, and the structure has boosted CYP1A2 activity. The integration of artificial cells and their living counter parts into larger 3D semi‐synthetic tissues is a step towards exploring bottom‐up synthetic biology in tissue engineering.This article is protected by copyright. All rights reserved
“…For many industrial processes, improvements to the selective separation and storage of gases can yield significant advantages . For example, the separation of CO 2 from air (N 2 and O 2 ) or natural gas (CH 4 ) has been of long running interest in the energy industry. , Another example is the Haber–Bosch process in which ammonia must be separated from unreacted nitrogen and hydrogen gas. This is typically done through condensation of the product ammonia from the reactant gases; however, this process can be costly to set up and operate.…”
Zeolites have tremendous potential as adsorbents for
the selective
separation and storage of gases in many industrial processes. The
extent and selectivity of gas adsorption are dependent on a number
of zeolite properties, such as pore diameter, accessible volume, and
silicon-to-aluminum ratio. In addition, the existence of aluminum
in the framework requires the presence of cations to maintain charge
neutrality, and the identity of these extra-framework cations will
also have a significant effect on adsorption. In this work, the continuous
fractional component Monte Carlo method was used to examine the effect
of the size and the charge of these cations on the adsorption of a
variety of gases (CH4, N2, and CO2) in two representative zeolite frameworks, clinoptilolite (heulandite)
(CLI) and RHO at 298.15 K, using a range of pressures from 0.1 to
60 bar for a variety of mono- and divalent cations (Na+, K+, Cs+, Mg2+, Ca2+, Sr2+, and Ba2+). Adsorption calculations
in the single-cation systems revealed unique trends in the size and
charge for each gas. To examine the combined effect of multiple cation
types, adsorption was calculated at 0.5 and 30 bar in several two-cation
systems, in which the effects of the size and charge were isolated
and examined. The resulting adsorption varied linearly with the mole
fraction of each two-cation mixture. A linear model is proposed to
describe the loading of gases in CLI and RHO zeolites containing complex
cation mixtures. Loading predicted by this model showed excellent
agreement with direct simulation of gas adsorption in randomized seven-cation
systems, suggesting that the adsorption effect of each cation is additive
among the zeolite frameworks tested.
“…CO 2 can be captured either at the source of emission or from the atmosphere. [1][2][3][4] One way of accomplishing this is by capturing carbon dioxide using simple chemical reagents, usually acting as bases. For instance, amines (e.g.…”
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