Reversing charges or how to improve Wharton's jelly mesenchymal stem cells culture on polyelectrolyte multilayer films

Rammal, Hassan; Beroud, Jacqueline; Gentils, Monique; Labrude, Pierre; Menu, Patrick; Kerdjoudj, Halima; Velot, Émilie

doi:10.3233/bme-130754

Cited by 11 publications

(10 citation statements)

References 42 publications

(66 reference statements)

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“…All procedures were done in accordance with the authorization and registration number DC-2014-2262 given by the French National "Cellule de Bioéthique". Stem cells, enzymatically isolated from fresh human umbilical cords obtained after full-term births [24], were cultured in complete culture medium [alpha-Minimal Eagle Medium (α-MEM, Lonza) supplemented with 10% decomplemented fetal bovine serum (FBS), 1% Penicillin/Streptomycin/Amphotericin B and 1% Glutamax® (v/v, Gibco)] and used in our experimental procedure at the 4th passage (P4).…”

Section: Biological Testsmentioning

confidence: 99%

Biocompatibility of sol-gel hydroxyapatite-titania composite and bilayer coatings

Sidane

Rammal

Beljebbar

et al. 2017

Materials Science and Engineering: C

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Titania-Hydroxyapatite (TiO/HAP) reinforced coatings are proposed to enhance the bioactivity and corrosion resistance of 316L stainless steel (316L SS). Herein, spin- and dip-coating sol-gel processes were investigated to construct two kinds of coatings: TiO/HAP composite and TiO/HAP bilayer. Physicochemical characterization highlighted the bioactivity response of the TiO/HAP composite once incubated in physiological conditions for 7days whereas the TiO/HAP bilayer showed instability and dissolution. Biological analysis revealed a failure in human stem cells adhesion on TiO/HAP bilayer whereas on TiO/HAP composite the presence of polygonal shaped cells, possessing good behaviour attested a good biocompatibility of the composite coating. Finally, TiO/HAP composite with hardness up to 0.6GPa and elastic modulus up to 18GPa, showed an increased corrosion resistance of 316L SS. In conclusion, the user-friendly sol-gel processes led to bioactive TiO/HAP composite buildup suitable for biomedical applications.

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Section: Biological Testsmentioning

confidence: 99%

Biocompatibility of sol-gel hydroxyapatite-titania composite and bilayer coatings

Sidane

Rammal

Beljebbar

et al. 2017

Materials Science and Engineering: C

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“…WJ-MSCs cultivated on PAH ending coating resulted in a disturbance of cell monolayer, whereas on PSS ending coating, WJ-MSCs exhibited a normal cell growth, suitable for endothelial cell differentiation. 33 PAH/PSS is a very-well-characterized multilayer system that has already been studied for biomedical applications.32 Figure 1(a) represents the evolution of the thickness of PAH/ PSS, measured by OWLS, for different number of bilayers. The alternated deposition of PAH and PSS allowed to increase the thickness of the film up to 15 nm for four bilayers.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, eight alternate PAH and PSS layers were deposited and (PAH-PSS) 4 films (PEMs) were then built up according to Ref. 33. Cleaned glass substrates coated with type-I collagen (COL-I, 100 lg/mL, BD-Bioscience) were used as control.…”

Section: Methodsmentioning

confidence: 99%

“…WJ-MSCs (n 5 5) were enzymatically isolated from fresh human umbilical cords obtained after full-term births according to Ref. 33. Both BM-and WJ-MSCs were cultured on 75 cm 2 plastic flask (Gibco) in alpha-Minimal Eagle Medium (a-MEM, Lonza) supplemented with 10% decomplemented fetal bovine serum (FBS), penicillin/streptomycin 100 IU/mL, Fungizone V R 2.5 mg/mL, and glutamine 2 mM and maintained in a humidified atmosphere of 5% CO 2 at 378C with a medium change every 2 days until use.…”

Section: Atomic Force Microscopymentioning

confidence: 99%

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Upregulation of endothelial gene markers in Wharton's jelly mesenchymal stem cells cultured on polyelectrolyte multilayers

Rammal

Harmouch

Maerten

et al. 2016

J Biomedical Materials Res

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Designing convenient substrates is a pertinent parameter that can guide stem cell differentiation. Current research is directed toward differentiating mesenchymal stem cells (MSCs) into endothelial cells (ECs). It is generally accepted that MSCs cannot be easily differentiated into ECs without high concentrations of proangiogenic factors. To guide either bone marrow-derived mesenchymal stem cells (BM-MSCs) and Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) into ECs-like phenotype, poly(allylamine-hydrochloride)/poly(styrene-sulfonate) multilayers film (PAH/PSS) was used as culture coating and compared to type I collagen (as control coating). After 2 weeks of culture and in absence of angiogenic growth factors, PAH/PSS upregulated KDR, PECAM-1, and CDH5 genes, whereas combining PAH/PSS with endothelial growth media (EGM-2 ) led to the production of respective proteins by WJ-MSCs. In contrast, not fully EC-like phenotype is obtained from the differentiation of BM- or WJ-MSCs cultured on type I collagen. Thus, using PAH/PSS coating in synergy with EGM-2 appears as an ideal condition promoting WJ-MSCs differentiation into ECs-like. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 292-300, 2017.

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“…20,21 Previsous studies in our lab have also demonstrated that poly(allylamine hydrochloride) (PAH) and poly(styrene sulfonate) (PSS) polyelectrolyte multilayer films improve culturing of WJ-MSCs and ECs. 22,23 We therefore used these two stimuli alone and in combination, to induce endothelial differentiation of WJ-MSCs. The obtained data showed that the differentiation into an endothelial phenotype was induced by cultivating WJ-MSCs at the fourth passage, seeded on (PAH/PSS) 4 film-coated glass slides, in the presence of EGM-2.…”

Section: Wj-mscs Acquire Endothelial Phenotype and Function On Differmentioning

confidence: 99%

Immunomodulation of endothelial differentiated mesenchymal stromal cells: impact on T and NK cells

et al. 2015

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Wharton's jelly mesenchymal stromal cells (WJ-MSCs) are promising candidates for tissue engineering, as their immunomodulatory activity allows them to escape immune recognition and to suppress several immune cell functions. To date, however, few studies have investigated the effect of differentiation of the MSCs on this immunomodulation. To address this question, we sought to determine the impact of differentiation toward endothelial cells on immunoregulation by WJ-MSCs. Following differentiation, the endothelial-like cells (ELCs) were positive for CD31, vascular endothelial cadherin and vascular endothelial growth factor receptor 2, and able to take up acetylated low-density lipoproteins. The expression of HLA-DR and CD86, which contribute to MSCs immunoprivilege, was still weak after differentiation. We then co-cultured un- and differentiated MSCs with immune cells, under conditions of both direct and indirect contact. The proliferation and phenotype of the immune cells were analyzed and the mediators secreted by both ELCs and WJ-MSCs quantified. Interleukin (IL)-6, IL-1β, prostaglandin E2 and in particular indoleamine-2,3-dioxygenase expression were upregulated in ELCs on stimulation by T and NK cells, suggesting the possible involvement of these factors in allosuppression. ELCs co-cultured with T cells were able to generate CD25(+) T cells, which were shown to be of the CD4(+)CD25(+)FoxP3(+) regulatory subset. Direct contact between NK cells and ELCs or WJ-MSCs decreased the level of NK-activating receptor natural-killer group 2, member D. Moreover, direct co-culturing with ELCs stimulates CD73 acquisition on NK cells, a mechanism which may induce adenosine secretion by the cells and lead to an immunosuppressive function. Taken together, our results show that ELCs obtained following differentiation of WJ-MSCs remain largely immunosuppressive.

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Reversing charges or how to improve Wharton's jelly mesenchymal stem cells culture on polyelectrolyte multilayer films

Cited by 11 publications

References 42 publications

Biocompatibility of sol-gel hydroxyapatite-titania composite and bilayer coatings

Biocompatibility of sol-gel hydroxyapatite-titania composite and bilayer coatings

Upregulation of endothelial gene markers in Wharton's jelly mesenchymal stem cells cultured on polyelectrolyte multilayers

Immunomodulation of endothelial differentiated mesenchymal stromal cells: impact on T and NK cells

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