Reversible Testis Injury in the Vitamin E-deficient Hamster

Mason, Karl E.; Mauer, Sidney I.

doi:10.1093/jn/105.4.484

Cited by 13 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Like vitamins A and B12, antioxidant vitamins ( vitamins C and E) are also essential for normal sperma-togenesis judging from evidence that animals de®cient in the vitamins exhibit spermatogenic disorders (Mason, 1933(Mason, , 1940Mason & Mauer, 1975;Chinoy et al, 1986;Sapra et al, 1987;Bensoussan et al, 1998). In the present study, we demonstrated that the simultaneous administration of vitamins C and E signi®cantly improved the spermatogenic Figure 4.…”

Section: Discussionmentioning

confidence: 99%

“…Although the mechanism underlying the testicular damage caused by DEHP remains unclear, it was recently found that the administration of vitamin B12 is effective in preventing DEHP-induced spermatogenic disturbance in rats (Oishi, 1994). Like vitamin B12, vitamins A, C and E are also essential for normal spermatogenesis (Mason, 1933(Mason, , 1940Coward et al, 1966;Mason & Mauer 1975;Chinoy et al, 1986;Sapra et al, 1987;Bensoussan et al, 1998). This raises the possibility that the administration of these vitamins would also have some bene®cial effect on spermatogenesis in DEHP-treated rats.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Spermatogenic disturbance induced by di‐(2‐ethylhexyl) phthalate is significantly prevented by treatment with antioxidant vitamins in the rat

et al. 2000

View full text Add to dashboard Cite

Phthalate esters, now regarded as endocrine disruptors, are widely used in the plastics industry. In particular, di-(2-ethylhexyl) phthalate (DEHP) is produced in large quantities, and is used in blood storage bags, catheters and haemodialysis instruments. Previous studies have demonstrated that treatment of rats with DEHP induces testicular atrophy with liver enlargement, although the precise nature and mechanism of the action of DEHP on these organs remains unclear. In the present study, we produced an experimental model of DEHP-induced spermatogenic disturbance in rats by feeding them a DEHP-containing diet. Liver enlargement occurred in rats fed either a 1 or 2% DEHP-containing diet. However, testicular atrophy accompanied by aspermatogenesis was induced by feeding with the 2% but not with the 1% DEHP-containing diet. This suggests that the critical DEHP dose for gonadotoxicity is higher than that for hepatotoxicity. Using the 2% DEHP-dose, the effect of simultaneous administration of antioxidant vitamins (= vitamins C and E) was next examined. It was found that the vitamin supplementation significantly prevented the testicular injury. The results suggest that antioxidant vitamins can protect the testes from DEHP-toxicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spermatogenic disturbance induced by di‐(2‐ethylhexyl) phthalate is significantly prevented by treatment with antioxidant vitamins in the rat

et al. 2000

View full text Add to dashboard Cite

show abstract

“…h Supplied per kilogram of diet for period 2: 55 mg of chlortetracycline. Vitamin E deficiencies caused testicular degeneration in chickens, rats, hamsters, rabbits, guinea pigs, dogs, cats, pigs, and monkeys and resulted in a lower number of germ cells and a reduction in sperm production (Mason, 1926(Mason, , 1933(Mason, , 1940(Mason, , 1954Mason and Maner, 1957;Cooper et al, 1987). Brzezinska-Slebodzinska et al (1995) suggested that dietary vitamin E may serve as an antioxidant in boar semen.…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary selenium and vitamin E on boar performance and tissue responses, semen quality, and subsequent fertilization rates in mature gilts.

Marin-Guzman

Mahan

Chung

et al. 1997

Journal of Animal Science

143

102

View full text Add to dashboard Cite

Three experiments involving 192 crossbred boars evaluated the effects of dietary Se (0 or .5 ppm) and vitamin E (0 or 220 IU/kg) on growth, tissue Se, and alpha-tocopherol concentrations, and on semen quality and its subsequent effect on fertilization rate in mature gilts. Diets formulated used torula yeast and dextrose or cornstarch as the basal feedstuffs and were provided from weaning through sexual maturity. The basal diets averaged .063 ppm Se and 3.46 mg alpha-tocopherol/kg diet. Experiment 1 was a 2 x 2 factorial and conducted as a randomized complete block design in six replicates. Boars were allotted at weaning (initial BW 7.7 kg) with growth and feed performance determined to 145 kg BW. Five boars were killed at weaning and three from each treatment group at periodic intervals to 145 kg BW. Serum and tissue Se and alpha-tocopherol concentration and glutathione peroxidase (GSH-Px) activity were subsequently determined. No performance benefit from either nutrient was demonstrated. Tissue (serum, liver, and testis) GSH-Px activity and Se and alpha-tocopherol concentrations were higher (P < .01) at each period when that respective nutrient fortified the diet. Testis GSH-Px activity increased from weaning to 145 kg BW even when Se was not added to the diet. Experiment 2 was conducted after training three boars from each treatment group of Exp. 1 for semen collection. From 9 mo of age and for a 16-wk period, semen was collected three times weekly and the volume, sperm concentration, motility, and percentage of normal and abnormal sperm were determined. Boars fed either the nonfortified Se or vitamin E diets had sperm with lower motilities (P < .01) and a higher percentage of sperm cells with bent and shoehook tails (P < .01). Diets low in added Se seemed to have a greater detrimental effect on the percentage of motile and abnormal sperm than diets inadequate in vitamin E. Sperm cells had a high concentration of Se and alpha-tocopherol, and a high GSH-Px activity. Experiment 3 was conducted using the boars from Exp. 2; 34 mature gilts were inseminated at 12 and 24 h after estrus. Gilts were killed 5 to 7 d postcoitum and the reproductive tracts were recovered. The semen from boars fed the nonfortified Se diet had a lower fertilization rate of oocytes with fewer accessory sperm penetrating the zona pellucida. The results from these experiments indicate that dietary Se and vitamin E can affect boar semen quality, but the greater effect seemed to be from Se.

show abstract

“…The novelty of our data consists of the fact that antioxidant vitamin supplements (as vitamin A and vitamin E) prolong the reproductive capacity of older roosters, suggesting the possibility of prolonging their reproductive contributions to the flock. Manson and Mauer (1974) revealed a remarkable regenerative response of seminiferous epithelium in hamsters experimentally deficient in vitamin E: when the degeneration reached quite advanced stages, the restoration of the germinal epithelium in most tubules was good, as a result of administration of vitamin E, but a variable number of tubules showed only limited repair. Similarly, in rats, Bensoussan et al (1998) showed that reintroducing dietary vitamin E to deficient rats restored a normal appearance of the structure of the testicle and epididymis, suggesting reversibility of the effects of the deficiency.…”

Section: Resultsmentioning

confidence: 98%

Comparative Effects of Vitamin A and E Dietary Supplements on Semen Traits, Reproductive System Morphology and Vitamin Storage in Rooster

ȘIPOTENU,

MOGOȘ,

CIOBOTARU

et al. 2023

BUASVMCN-VM

View full text Add to dashboard Cite

The purpose of this study was to determine the effect of long-term vitamin A and E dietary supplements on the semen traits and morphology of the reproductive system in older roosters to prolong their reproductive contributions to the flock. The research revealed that long-term dietary supplementation of both vitamins A and E maintained the traits of the semen at levels significantly higher than in the control roosters. Vitamin A especially maintained ejaculate volume and semen density, while vitamin E protected semen motility and viability. Vitamin A treatment maintained the uniformity of the epididymal epithelium. No eroded areas were identified in this epithelium. Most epithelial cells were principal cells with uniform stereocilia. The connective tissue which lines the epididymal tubule of the vitamin A treated rooster was thinner than those of the roosters from the control. Vitamin E predominantly protected the structure of the spermatogenesis line. Liver tissue concentrations of vitamins A and E were two times and four times higher respectively than the control. The two vitamins were eliminated in increased quantities through the seminal plasma in supplemented roosters. No mutual inhibition or potentiating effects were found between the two vitamins, suggesting that their combined administration should be further analyzed.

show abstract

Reversible Testis Injury in the Vitamin E-deficient Hamster

Cited by 13 publications

References 6 publications

Spermatogenic disturbance induced by di‐(2‐ethylhexyl) phthalate is significantly prevented by treatment with antioxidant vitamins in the rat

Spermatogenic disturbance induced by di‐(2‐ethylhexyl) phthalate is significantly prevented by treatment with antioxidant vitamins in the rat

Effects of dietary selenium and vitamin E on boar performance and tissue responses, semen quality, and subsequent fertilization rates in mature gilts.

Comparative Effects of Vitamin A and E Dietary Supplements on Semen Traits, Reproductive System Morphology and Vitamin Storage in Rooster

Contact Info

Product

Resources

About