Spermatogenic disturbance induced by di‐(2‐ethylhexyl) phthalate is significantly prevented by treatment with antioxidant vitamins in the rat

Ishihara, Masaru; Itoh, Masahiro; Miyamoto, Kensaku; Suna, Shigeru; Takeuchi, Yoshiki; Takenaka, Ikumasa; Jitsunari, Fumihiko

doi:10.1046/j.1365-2605.2000.00212.x

Cited by 73 publications

(48 citation statements)

References 40 publications

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“…Curcumin and kolaviron may thus prevent peroxidative changes in the sperm and the testicular membrane, thus enhancing sperm motility and decreasing spermatozoa abnormalities. Our results are in agreement with Ishihara et al [5] who reported the protective effect of antioxidant vitamins on di-ethyl-hexylphthalate-induced testicular atrophy, a similar phthalate ester. In addition, co-administration of kolaviron and curcumin might have relieved the inhibition of the antioxidant enzymes, reduction of testosterone, G6PD and γ-GT, and/or may have triggered their synthesis that in turn attenuated the oxidative damage caused by DBP or its metabolites.…”

Section: Discussionsupporting

confidence: 94%

“…The exact mechanisms involved in the formation of phthalate-induced testicular atrophy are not known, but recently it was shown that administration of antioxidant vitamins together with di-ethyl-hexylphthalate prevented phthalate ester-induced testicular atrophy in rats [5] suggesting that the formation of reactive oxygen species may be involved in the formation of testicular atrophy in phthalate-exposed rats.…”

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confidence: 99%

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Curcumin and Kolaviron Ameliorate Di‐n‐Butylphthalate‐Induced Testicular Damage in Rats

Farombi

Abarikwu

Adedara

et al. 2006

Basic Clin Pharma Tox

119

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The present study was carried out to evaluate the ameliorative effects of kolaviron (a biflavonoid from the seeds of Garcinia kola ) and curcumin (from the rhizome, Curcuma longa L.) on the di-n-butylphthalate (DBP)-induced testicular damage in rats. Administration of DBP to rats at a dose of 2 g / kg for 9 days significantly decreased the relative testicular weights compared to the controls, while the weights of other organs remained unaffected. Curcumin or kolaviron did not affect all the organ weights of the animals. While only DBP treatment significantly increased the testicular malondialdehyde level and gamma-glutamyl transferase activity ( γ -GT), it markedly decreased glutathione level, the testicular catalase, glucose-6-phosphate dehydrogenase, superoxide dismutase, sperm γ -GT activities and serum testosterone level compared to the control group. Data on cauda epididymal sperm count and live/dead ratio were not significantly affected in the DBPtreated rats. Alone, DBP treatment resulted in a 66% decrease in spermatozoa motility and a 77% increase in abnormal spermatozoa in comparison to control. DBP-treated rats showed marked degeneration of the seminiferous tubules with necrosis and defoliation of spermatocytes. The DBP-induced injuries in biochemical, spermatological parameters and histological structure of testis were recovered by treatment with kolaviron or curcumin. The pattern in the behaviour of these compounds might be correlated with their structural variations. Our results indicate that kolaviron and curcumin protect against testicular oxidative damage induced by DBP. The chemoprotective effects of these compounds may be due to their intrinsic antioxidant properties and as such may prove useful in combating phthalate-induced reproductive toxicity.Benzenedicarboxylic acid dibutyl ester, also known as butylphthalate or di-n-butylphthalate (DBP) ( fig. 1A), is widely used as a plasticizer in cellulose plastics, as a solvent for dye, and for a variety of consumer products [1]. Release of DBP to the environment can occur during production and also during incorporation of the phthalate into plastics. Because DBP is not bound to the final product, it can be released during use or disposal of the product. Thus, the general population is exposed to phthalates through consumer products, diet and medical devices [2].Phthalate esters have been implicated in the decline of human sperm counts [3] and testicular atrophy in an animal model [4]. The exact mechanisms involved in the formation of phthalate-induced testicular atrophy are not known, but recently it was shown that administration of antioxidant vitamins together with di-ethyl-hexylphthalate prevented phthalate ester-induced testicular atrophy in rats [5] suggesting that the formation of reactive oxygen species may be involved in the formation of testicular atrophy in phthalate-exposed rats.Curcumin obtained from the Curcuma longa rhizome is the major component of turmeric used as a spice to give the specific flavour and yellow colour to cu...

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Section: Discussionsupporting

confidence: 94%

mentioning

confidence: 99%

Curcumin and Kolaviron Ameliorate Di‐n‐Butylphthalate‐Induced Testicular Damage in Rats

Farombi

Abarikwu

Adedara

et al. 2006

Basic Clin Pharma Tox

119

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“…Such products contain numerous chemicals, at least two of which (various types of parabens or phthalates) have been implicated in potentially causing adverse effects on testosterone and/or sperm production in animal studies (e.g. David et al 2000;Ishihara et al 2000;Oishi 2001Oishi , 2002, although for phthalates such effects have only been found after exposure to extremely high levels (500 -1500 mg kg 21 d…”

Section: Effects Of Environmental Chemicals On Spermatogenesis In Adumentioning

confidence: 99%

Environmental/lifestyle effects on spermatogenesis

Sharpe

2010

Phil. Trans. R. Soc. B

296

198

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The high incidence of low sperm counts in young (European) men and evidence for declining sperm counts in recent decades mean that the environmental/lifestyle impact on spermatogenesis is an important health issue. This review assesses potential causes involving adverse effects on testis development in perinatal life (primarily effects on Sertoli cell number), which are probably irreversible, or effects on the process of spermatogenesis in adulthood, which are probably mainly reversible. Several lifestyle-related (obesity, smoking) and environmental (exposure to traffic exhaust fumes, dioxins, combustion products) factors appear to negatively affect both the perinatal and adult testes, emphasizing the importance of environmental/lifestyle impacts throughout the life course. Apart from this, public concern about adverse effects of environmental chemicals (ECs) (pesticides, food additives, persistent pollutants such as DDT, polychlorinated biphenyls) on spermatogenesis in adult men are, in general, not supported by the available data for humans. Where adverse effects of ECs have been shown, they are usually in an occupational setting rather than applying to the general population. In contrast, a modern Western lifestyle (sedentary work/lifestyle, obesity) is potentially damaging to sperm production. Spermatogenesis in normal men is poorly organized and inefficient so that men are poorly placed to cope with environmental/lifestyle insults.

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“…The treatment with the water extract of cumin plant (200 mg/kg B.W.) showed a significant decrease in testicular lipid hormone compared with the control group [31]. Effect of different treatments of the total content of sperms in the epididymis: It is noted from Figure (4) that there is a significant decrease in the treatment group with H2O2, the total number of sperm compared with the control.…”

Section: Tikrit Journal Of Pure Science 23 (3) 2018 Issn: 1813 -1662 mentioning

confidence: 91%

“…showed that the sperm cells were very effective compared to the control group (fig. 5-G &H), this may be due to the active substances contained in this plant that have resulted in a nonpositive result [31,33]. The result of histological section of the testes in the rats treated with vitamin E and H2O2 observed repair of the cells lining the seminiferous tubules with the continuation of the formation of sperm as shown in the figure (5-I&J) and this is agree with the result of [35] which observed that rats treated with vitamin E with fluoxetine showed an improvement in semeniferous tubules and an increase in the number of sperm.…”

Section: Histological Effect Of Different Treatment On Testismentioning

confidence: 99%

Physiological and histological study of the effect of Cumin Cyminum watery extract and vitamin E on the male reproductive system in rats exposed to oxidative stress

2018

Tikrit J. Pure Sci.

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This study was conducted to investigate the effect of watery extract of Cumin plant (200 mg/kg B.W.) on some physiological and histological parameters of the male reproductive system in white albino rats exposed to oxidative stress by Hydrogen peroxide (H2O2 0.5%). Thirty adult male rats weighting about (200-250 g) and 10-12 weeks age were used in the present study and divided into (5) groups, 6 rats per group, group1: serve as a control group and given normal diet with drinking water, group2: Administrated H2O2 0.5% with drinking water, group3: Given Cumin extract (200 mg/kg B.W.) through oral tubular feeding + H2O2 (0.5%), group4: Given Cumin extract (200 mg/kg B.W.) alone and group5: Administrated Vitamin E 500 (mg/kg B.W.) with H2O2 0.5%, the experiment were continued 30 days for all groups. The results of the current study showed that treatment with H2O2 resulted significant increase (P<0.05) in malondialdehyde, significant decrease (P <0.05) in glutathione and significant decrease (P<0.05) in serum testosterone compared with control group, While the treatment with H2O2 plus the water extract of Cumin seeds (200 mg/kg B.W.) showed a significant decrease (P<0.05) in malondialdehyde, a significant increase in glutathione and a significant increase in testosterone in the serum compared to the H2O2 group. As for ectopic tissue, H2O2 has resulted in satisfactory tissue changes, while water extract (200 mg/kg) with H2O2 resulted in little improvement of the testes tissue. The water extract of Cumin plant acts as an antioxidant through its important role in curbing the harmful effects of some types of free radicals within the body and thus repair the damage in most of the physiological and histological changes and functions of the male reproductive system of rats with exposure to oxidative. Histological section of the testes in the rats treated with vitamin E and H2O2 observed repair of the cells lining the seminiferous tubules with the continuation of the formation of sperm, on the other hand the results showed that giving the Cumin for a long periods lead to reduce the level of hormone testosterone.

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Spermatogenic disturbance induced by di‐(2‐ethylhexyl) phthalate is significantly prevented by treatment with antioxidant vitamins in the rat

Cited by 73 publications

References 40 publications

Curcumin and Kolaviron Ameliorate Di‐n‐Butylphthalate‐Induced Testicular Damage in Rats

Curcumin and Kolaviron Ameliorate Di‐n‐Butylphthalate‐Induced Testicular Damage in Rats

Environmental/lifestyle effects on spermatogenesis

Physiological and histological study of the effect of Cumin Cyminum watery extract and vitamin E on the male reproductive system in rats exposed to oxidative stress

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