Reversible photocontrol of oxidase activity by inserting a photosensitive domain into the oxidase

Abstract

Background: Photocontrol of protein activity has become a helpful strategy for regulating biological pathways. Herein, a method for the precise and reversible photocontrol of oxidase activity was developed by using the conformational change of the AsLOV2 domain. Results: The AsLOV2 domain was inserted into the nonconserved sites exposed on the surface of the AdhP protein, and the alov9 fusion was successfully screened for subsequent optical experiments under the assumption that neither of these actions affected the original activity of AdhP protein. The activity of alov9 was noticeably inhibited when the fusion was exposed to 470 nm blue light and recovered within 30 min. As a result, we could precisely and reversibly photocontrol alov9 activity through the optimization of several parameters, including cofactor concentration, light intensity, and illumination time. Conclusions: An efficient method was developed for the photoinhibition of enzymatic activity based on the insertion of the light-sensitive AsLOV2 domain, providing new ideas for photocontrolling metabolic pathways without carriers in the future.

Bioethylene Production from Ethanol: A Review and Techno‐economical Evaluation

“…Lignin forms a protective barrier for the cellulose microfibrils, which makes the cellulose hydrolysis to glucose difficult. In addition, the linkage between lignin and hemicellulose hinders the degradation of hemicellulose to pentose and hexose sugars [38,45]. The process of ethanol production from lignocellulosic biomass consists of four main steps, namely, pretreatment, hydrolysis, fermentation, and distillation [46].…”

“…The process of ethanol production from lignocellulosic biomass consists of four main steps, namely, pretreatment, hydrolysis, fermentation, and distillation [46]. In the pretreatment step, the lignocellulosic structure is opened up via physical, chemical, physicochemical, or biological methods [45]. Hydrolysis is the second step where the cellulose and hemicellulose molecules are degraded into monosaccharides by cellulolytic enzymes or acids [46].…”

Benchmarking of density functionals for the kinetics and thermodynamics of the hydrolysis of glycosidic bonds catalyzed by glycosidases

“…[6,7] These reactions can happen between two carbohydrates or between a carbohydrate and a noncarbohydrate moiety (amino acids, e.g., in glycoproteins). [7,8] Due to the critical role of carbohydrates in human health [9][10][11] as well as their possible use as fuel, [12][13][14][15][16][17] there has been an increased interest in the study of glycosidases in recent years. [18][19][20][21][22][23][24][25][26][27][28] Glycosidases are very abundant in nature.…”

Rapid mechanoenzymatic saccharification of lignocellulosic biomass without bulk water or chemical pre-treatment

“…With the recognition that use of fossil resources is unsustainable, lignocellulosic biomass has been identified as a main candidate to fulfil the future needs for fuels and basic chemicals. [1][2][3][4][5][6] Saccharification of the biopolymer constituents of biomass affords monosaccharides -mainly glucose and xylose -which have been recognized as convenient platforms for the production of valued molecules such as ethanol, 7 polyhydroxyalkanoates, [8][9][10] succinate, 11 and itaconate. 12 Despite being produced at an estimated rate of 10 12 ton per year, 13 lignocellulosic materials such as agricultural and forestry wastes remain underexploited because of their poor solubility and remarkably recalcitrant nature.…”