Reversible pH Responsive Bovine Serum Albumin Hydrogel Sponge Nanolayer

Raghuwanshi, Vikram Singh; Yu, Brendan; Browne, Christine; Garnier, Gil

doi:10.3389/fbioe.2020.00573

Cited by 41 publications

(30 citation statements)

References 36 publications

(41 reference statements)

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“…The carrier protein BSA was encapsulated within all nanoparticle formulations where a 40−65% encapsulation efficiency was achieved, as determined with a BCA protein assay (Figure S2A). In addition, we found that BSA, which is negatively charged at physiological pH, 38 contributed moderately to the negative charge of the nanoparticles when encapsulated therein (Figure S2B). As we decreased the molecular weight of PLGA, the zeta potential of the nanoparticles became more negative.…”

Section: ■ Results and Discussionmentioning

confidence: 95%

Tunable Surface Charge Enables the Electrostatic Adsorption-Controlled Release of Neuroprotective Peptides from a Hydrogel–Nanoparticle Drug Delivery System

Deng

Akbar

et al. 2022

ACS Appl. Mater. Interfaces

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We exploit the electrostatic interactions between the positively charged neuroprotective peptide, pituitary adenylate cyclase-activating polypeptide (PACAP), and negatively charged poly(lactic-co-glycolic acid) (PLGA) nanoparticles to control PACAP release from the surface of nanoparticles dispersed in a hyaluronan-methylcellulose (HAMC) hydrogel composite. PACAP is a promising therapeutic for the treatment of neurological disorders, yet it has been difficult to deliver in vivo. Herein, the PACAP release rate was tuned by manipulating peptide adsorption onto the surface of blank nanoparticles by modifying either nanoparticle loading in the hydrogel or nanoparticle surface charge. This peptide–nanoparticle interaction was controlled by the pH-responsive carboxylic acid end terminal groups of PLGA. We further validated this system with the controlled release of a novel stabilized PACAP analog: Ac-[Ala15, Ala20]PACAP-propylamide, which masks its recognition to peptidases in circulation. Both wild-type and stabilized PACAP released from the vehicle increased the production of neuroprotective Interleukin-6 from cultured primary astrocytes. Using computational fluid dynamics methods, PACAP release from the composite was predicted based on experimentally derived adsorption isotherms, which exhibited similar release profiles to experimental data. This versatile adsorption-based system was used to deliver PACAP locally to the brains of stroke-injured mice over a 10 day period in vivo, highlighting its effectiveness for the controlled release of PACAP to the central nervous system.

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Section: ■ Results and Discussionmentioning

confidence: 95%

Tunable Surface Charge Enables the Electrostatic Adsorption-Controlled Release of Neuroprotective Peptides from a Hydrogel–Nanoparticle Drug Delivery System

Deng

Akbar

et al. 2022

ACS Appl. Mater. Interfaces

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“…When analyzing the conductivity characteristics of the considered solution, we may conclude that it is a strong electrolyte. It follows from the pH value of BSA (4.5–4.8) [ 21 ] that was primarily added when obtaining the CLEA. Hence, we can use Kohlrausch’s law for a solution of a strong electrolyte:

…”

Section: Resultsmentioning

confidence: 99%

Nonlinear Analytics for Electrochemical Biosensor Design Using Enzyme Aggregates and Delayed Mass Action

Martsenyuk

Kłos-Witkowska

Dzyadevych

et al. 2022

Sensors

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The paper is devoted to the extension of Brown’s model of enzyme kinetics to the case with distributed delays. Firstly, we construct a multi-substrate multi-inhibitor model using discrete and distributed delays. Furthermore, we consider simplified models including one substrate and one inhibitor, for which an experimental study has been performed. The algorithm of parameter identifications was developed which was tested on the experimental data of solution conductivity. Both the model and Kohlrausch’s law parameters are obtained as a result of the optimization procedure. Comparison of plots constructed with the help of the estimated parameters has shown that in such case the model with distributed delays is more chemically adequate in comparison with the discrete one. The methods of generalization of the results to the multi-substrate multi-inhibitor cases are discussed.

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“…As a model protein, BSA has an isoelectric point (IEP) of 4.5-4.8 which means under neutral pH condition, it has a negative molecular net charge. 50 However, PDGF has an IEP of 9.8 and turns to have a positive molecular net charge under the physiological milieu. 51 As the CS bioadhesives are positively charged from the amine groups, it is predictable that PDGF will have a faster-releasing profile compared to BSA due to the electrostatic force of repulsion which is beneficial to maintain a therapeutic concentration level within the dynamic homeostasis environment.…”

Section: Discussionmentioning

confidence: 99%

Biomolecules‐releasing click chemistry‐based bioadhesives for repairing acetabular labrum tears

Izuagbe

et al. 2022

Journal Orthopaedic Research

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Currently, there are no effective clinical or experimental treatments to fully restore the function of the torn acetabular labrum. To fill the gap, here, we report the finding of progenitor cells in labral tissue, which can be recruited and stimulated to repair torn acetabular labral tissue. This study aimed to develop a biomolecule releasing bioadhesive which can speed up labral tissue healing by eliciting autologous labral progenitor cellular responses. A click chemistry‐based bioadhesive, capable of releasing biomolecules, was synthesized to exert ~3× adhesion strength compared with fibrin glue. Via the release of platelet‐derived growth factor (PDGF), the adhesive was shown to actively recruit and stimulate the proliferation of labral progenitor cells to the tear sites and within the adhesive. Finally, the ability of this biomolecules‐releasing adhesive designed to promote labral tissue regeneration was evaluated using discarded human acetabular labrum tissue compared with surgical suture ex vivo. Histological analysis shows that PDGF‐releasing bioadhesive yielded significantly more labrum cell responses and extracellular matrix protein (proteoglycan and collagen) production at the tear tissue site than surgical suture controls. The results confirm that the new PDGF‐releasing bioadhesive can activate the responses of autologous labral progenitor cells to significantly improve labral tissue regeneration. Clinical significance: These PDGF‐releasing bioadhesives may serve as a new and effective tool for repairing and regenerating acetabular labrum tears.

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Reversible pH Responsive Bovine Serum Albumin Hydrogel Sponge Nanolayer

Cited by 41 publications

References 36 publications

Tunable Surface Charge Enables the Electrostatic Adsorption-Controlled Release of Neuroprotective Peptides from a Hydrogel–Nanoparticle Drug Delivery System

Tunable Surface Charge Enables the Electrostatic Adsorption-Controlled Release of Neuroprotective Peptides from a Hydrogel–Nanoparticle Drug Delivery System

Nonlinear Analytics for Electrochemical Biosensor Design Using Enzyme Aggregates and Delayed Mass Action

Biomolecules‐releasing click chemistry‐based bioadhesives for repairing acetabular labrum tears

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