Reversible inhibition of photophosphorylation in chloroplasts by nitric oxide

Takahashi, Shunichi; Yamasaki, Hideo

doi:10.1016/s0014-5793(02)02244-5

Cited by 113 publications

(73 citation statements)

References 32 publications

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“…Phosphorylation of PSII reaction center proteins D1 and D2, as well as other subunits of PSII, was found to be stimulated by moderately thiol-reducing conditions and kept at a high level also under highly reducing conditions (Carlberg et al, 1999). Furthermore, reversible inhibition of photophosphorylation in chloroplasts by NO was demonstrated (Takahashi and Yamasaki, 2002), suggesting that proteins of the energy transduction system in chloroplast thylakoids could be affected by S-nitrosylation.…”

Section: Discussionmentioning

confidence: 93%

Proteomic Identification of S-Nitrosylated Proteins in Arabidopsis

2005

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Although nitric oxide (NO) has grown into a key signaling molecule in plants during the last few years, less is known about how NO regulates different events in plants. Analyses of NO-dependent processes in animal systems have demonstrated protein S-nitrosylation of cysteine (Cys) residues to be one of the dominant regulation mechanisms for many animal proteins. For plants, the principle of S-nitrosylation remained to be elucidated. We generated S-nitrosothiols by treating extracts from Arabidopsis (Arabidopsis thaliana) cell suspension cultures with the NO-donor S-nitrosoglutathione. Furthermore, Arabidopsis plants were treated with gaseous NO to analyze whether S-nitrosylation can occur in the specific redox environment of a plant cell in vivo. S-Nitrosylated proteins were detected by a biotin switch method, converting S-nitrosylated Cys to biotinylated Cys. Biotin-labeled proteins were purified and analyzed using nano liquid chromatography in combination with mass spectrometry. We identified 63 proteins from cell cultures and 52 proteins from leaves that represent candidates for S-nitrosylation, including stress-related, redox-related, signaling/regulating, cytoskeleton, and metabolic proteins. Strikingly, many of these proteins have been identified previously as targets of S-nitrosylation in animals. At the enzymatic level, a case study demonstrated NO-dependent reversible inhibition of plant glyceraldehyde-3-phosphate dehydrogenase, suggesting that this enzyme could be affected by S-nitrosylation. The results of this work are the starting point for further investigation to get insight into signaling pathways and other cellular processes regulated by protein S-nitrosylation in plants.

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Section: Discussionmentioning

confidence: 93%

Proteomic Identification of S-Nitrosylated Proteins in Arabidopsis

2005

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“…It has been reported that photosynthesis can be affected not only by generation of reactive oxygen species (for review, see Asada, 1999), but also by reaction with NO and other NO-related species. Takahashi and Yamasaki (2002) have shown that NO is capable of inhibiting chloroplast electron transport in a reversible manner. Previously, studies of air pollution had suggested that NO may reduce CO 2 assimilation (Hill and Bennett, 1970).…”

mentioning

confidence: 99%

“…Previously, studies of air pollution had suggested that NO may reduce CO 2 assimilation (Hill and Bennett, 1970). Both photosynthesis and photorespiration have been found to be affected by NO in different plants (Yamasaki, 2000;Takahashi and Yamasaki, 2002). The aim of this work was to identify endogenous sources of NO generation and NO and ONOO 2 -dependent oxidative damage in isolated soybean (Glycine max) chloroplasts.…”

mentioning

confidence: 99%

Chloroplasts as a Nitric Oxide Cellular Source. Effect of Reactive Nitrogen Species on Chloroplastic Lipids and Proteins

Jasid

Simontacchi²,

Bártoli³

et al. 2006

Plant Physiology

279

139

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Nitric oxide (NO) generation by soybean (Glycine max var. ADM 4800) chloroplasts was studied as an endogenous product assessed by the electron paramagnetic resonance spin-trapping technique. Nitrite and L-arginine (Arg) are substrates for enzymatic activities considered to be the possible sources of NO in plants. Soybean chloroplasts showed a NO production of 3.2 6 0.2 nmol min 21 mg 21 protein in the presence of 1 mM NaNO 2 . Inhibition of photosynthetic electron flow by 3-(3,4-dichlorophenyl)-1,1-dimethyl urea resulted in a lower rate (1.21 6 0.04 nmol min 21 mg 21 protein) of NO generation. Chloroplasts incubated with 1 mM Arg showed NO production of 0.76 6 0.04 nmol min 21 mg 21 protein that was not affected either by omission of Ca 21 or by supplementation with Ca 21 and calmodulin to the incubation medium. This production was inhibited when chloroplasts were incubated in the presence of NO synthase inhibitors N v -nitro-L-Arg methyl ester hydrochloride and N v -nitro-L-Arg. In vitro exposure of chloroplasts to an NO donor (250 mM S-nitrosoglutathione) decreased lipid radical content in membranes by 29%; however, incubation in the presence of 25 mM peroxynitrite (ONOO 2 ) led to an increase in lipid-derived radicals (34%). The effect of ONOO 2 on protein oxidation was determined by western blotting, showing an increase in carbonyl content either in stroma or thylakoid proteins as compared to controls. Moreover, ONOO 2 treatment significantly affected both O 2 evolution and chlorophyll fluorescence in thylakoids. Data reported here suggest that NO is an endogenous metabolite in soybean chloroplasts and that reactive nitrogen species could exert either antioxidant or prooxidant effects on chloroplast macromolecules.

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“…NO is a lipophilic molecule that permeates the membrane and is an important signal molecule in plants growing under biotic and abiotic stresses (Tan et al 2008). NO is reported to induce SG (Beligni and Lamattina 2000), regulate plant metabolism (Leshem et al 1998) and photosynthesis (Takahashi & Yamasaki 2002), and alleviate the effect of oxidative stress (Beligni and Lamattina 1999;. Seed germination was greatly affected by BA with a maximum decrease in BA 3 .…”

Section: Resultsmentioning

confidence: 99%

Positive effects of nitric oxide onSolanum lycopersicum

Singh

Yadav

Amist

2012

Journal of Plant Interactions

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The present work examines benzoic acid (BA) induced stress in Solanum lycopersicum and explores the possible mechanism through which sodium nitroprusside (SNP) protects plants. Tomato seeds were soaked for 3 h in 250 mM SNP and 0.5, 1.0, and 1.5 mM BA with and without SNP. Seeds soaked in distilled water were treated as control. Seed germination (SG) and radicle length (RL) were recorded in Petri plate culture. SG and RL inhibition was concentration dependent in BA. SNP enhanced SG and RL. Twenty-one-days old seedlings were grown in hydroponic culture in Hoagland solution and BA at 0.5, 1.0, and 1.5 mM with and without SNP. The morphological and biochemical parameters of seedlings were assayed. Average growth rate of root and shoot, dry weight, sugar, pigment and protein content, and activity of nitrate reductase decreased in seedlings treated with BA while increased in SNP with and without BA. Lipid peroxidation increased in seedlings treated with BA but decreased in BA 'SNP. SNP protected the seedlings against BA induced oxidative stress by scavenging reactive oxygen species. SNP demonstrated a positive role against BA toxicity which was evident from increased activities of antioxidant enzymes.

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Reversible inhibition of photophosphorylation in chloroplasts by nitric oxide

Cited by 113 publications

References 32 publications

Proteomic Identification of S-Nitrosylated Proteins in Arabidopsis

Proteomic Identification of S-Nitrosylated Proteins in Arabidopsis

Chloroplasts as a Nitric Oxide Cellular Source. Effect of Reactive Nitrogen Species on Chloroplastic Lipids and Proteins

Positive effects of nitric oxide onSolanum lycopersicum

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