Reversible dougong structured receptor–ligand recognition for building dynamic extracellular matrix mimics

Abstract

Dynamic biomaterials excel at recapitulating the reversible interlocking and remoldable structure of the extracellular matrix (ECM), particularly in manipulating cell behaviors and adapting to tissue morphogenesis. While strategies based on dynamic chemistries have been extensively studied for ECM-mimicking dynamic biomaterials, biocompatible molecular means with biogenicity are still rare. Here, we report a nature-derived strategy for fabrication of dynamic biointerface as well as a three-dimensional (3D) hydrogel structure based on reversible receptor–ligand interaction between the glycopeptide antibiotic vancomycin and dipeptide d-Ala-d-Ala. We demonstrate the reversible regulation of multiple cell types with the dynamic biointerface and successfully implement the dynamic hydrogel as a functional antibacterial 3D scaffold to treat tissue repair. In view of the biogenicity and high applicability, this nature-derived reversible molecular strategy will bring opportunities for malleable biomaterial design with great potential in biomedicine.

A dynamic nano-coordination protein hydrogel for photothermal treatment and repair of infected skin injury

“…Skin tissue sections were also applied for haematoxylin and eosin (H&E) and Masson's trichrome staining. 15 As shown in Fig. 3I and Fig.…”

“…Skin sections of the injected region were further applied for immunofluorescence staining against S. aureus after treatment. 15 As shown in Fig. 3D, skin sections in the control and the hydrogel group without NIR irradiation showed strong green fluorescence ( i.e.…”

Reversible Self-Assembled Monolayers with Tunable Surface Dynamics for Controlling Cell Adhesion Behavior

“…Integrins, the main cellular receptors for the extracellular matrix, have a key role in mediating these activities. , The tripeptide Arg-Gly-Asp or RGD is one of the highly conserved peptide sequences present in the extracellular matrix (ECM) recognized by the integrins. Since its discovery, this peptide sequence and its variations have been integrated into and onto a variety of scaffolds to investigate the role of cell adhesion molecules during cell adhesion processes and fabrication of biomaterials for cell culture, tissue engineering, and regenerative medicine. − The scaffolds for immobilizing bioactive peptides can be either static (biopolymers, self-assembled monolayers (SAMs) − ) or dynamic (hydrogels, , supported lipid bilayers (SLBs), host–guest-based assemblies, − self-assembled peptide amphiphiles). In terms of two-dimensional (2D) crystalline-like layers, the most well-studied cases are SAMs and SLBs. ,,,, The former in combination with light-responsive, , magnetic, or electrical-responsive functionalities, ,, or host–guest-based chemistry, − enable controllable surface properties for reversible cell adhesion albeit featuring only short-range dynamic properties.…”

“…Since its discovery, this peptide sequence and its variations have been integrated into and onto a variety of scaffolds to investigate the role of cell adhesion molecules during cell adhesion processes and fabrication of biomaterials for cell culture, tissue engineering, and regenerative medicine. − The scaffolds for immobilizing bioactive peptides can be either static (biopolymers, self-assembled monolayers (SAMs) − ) or dynamic (hydrogels, , supported lipid bilayers (SLBs), host–guest-based assemblies, − self-assembled peptide amphiphiles). In terms of two-dimensional (2D) crystalline-like layers, the most well-studied cases are SAMs and SLBs. ,,,, The former in combination with light-responsive, , magnetic, or electrical-responsive functionalities, ,, or host–guest-based chemistry, − enable controllable surface properties for reversible cell adhesion albeit featuring only short-range dynamic properties. The latter, however, are characterized by their long-range lateral mobility, which is conducive to integrin clustering required for downstream signal transduction paths for cell growth and differentiation .…”

“…Since its discovery, this peptide sequence and its variations have been integrated into and onto a variety of scaffolds to investigate the role of cell adhesion molecules during cell adhesion processes and fabrication of biomaterials for cell culture, tissue engineering, and regenerative medicine. 7−14 The scaffolds for immobilizing bioactive peptides can be either static (biopolymers, 15 self-assembled monolayers (SAMs) 16−18 ) or dynamic (hydrogels, 19,20 supported lipid bilayers (SLBs), 11 host−guest-based assemblies, 19−22 self-assembled peptide amphiphiles 23 ). In terms of two-dimensional (2D) crystalline-like layers, the most well-studied cases are SAMs and SLBs.…”