Reversible and Noncompetitive Inhibition of β‐Tryptase by Protein Surface Binding of Tetravalent Peptide Ligands Identified from a Combinatorial Split–Mix Library

Wich, Peter; Schmuck, Carsten

doi:10.1002/anie.200907221

Cited by 27 publications

(4 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Magnetically labeled objects consequently cause the NMR signal to decay faster, leading to a shorter T 2 , than non-targeted objects. The detection sensitivity of DMR has been significantly enhanced in recent years by miniaturizing NMR probes 7,16 and synthesizing highly magnetic nanoparticles. 8 …”

Section: Methodsmentioning

confidence: 99%

“…One fundamental hurdle for bringing DMR out of the laboratory has been its inherent sensitivity to temperature, which originates from the temperature-dependent fluctuation of the magnetic field ( B ) of the permanent magnet in the system. 16 The drift in B leads to corresponding changes in the Larmor frequency ( f 0 ) of the NMR signal, causing artifacts in the measured T 2 values. In a laboratory setting, the problem can be addressed by controlling the environmental and system temperatures.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Miniature magnetic resonance system for point-of-care diagnostics

et al. 2011

View full text Add to dashboard Cite

We have developed a next generation, miniaturized platform to diagnose disease at the point-of-care using diagnostic magnetic resonance (DMR-3). Utilizing a rapidly growing library of functionalized magnetic nanoparticles, DMR has previously been demonstrated as a versatile tool to quantitatively and rapidly detect disease biomarkers in unprocessed biological samples. A major hurdle for bringing DMR to the point-of-care has been its sensitivity to temperature variation. As an alternative to costly and bulky mechanisms to control temperature, we have implemented an automated feedback system to track and compensate for the temperature drift, which enables reliable and robust DMR measurements in realistic clinical environments (4–50 °C). Furthermore, the new system interfaces with a mobile device to facilitate system control and data sharing over wireless networks. With such features, the DMR-3 platform can function as a self-contained laboratory even in resource-limited, remote settings. The clinical potential of the new system is demonstrated by detecting trace amounts of proteins and as few as 10 bacteria (Staphylococcus aureus) in a short time frame (<30 min).

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Miniature magnetic resonance system for point-of-care diagnostics

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Recently,L ee et al reported pyrene-based peptidyl chemosensors for ratiometric detection for heparin but this systema lso suffers from poor selectivity. [14e] As part of al arger program in our laboratory aimed at developing receptors and sensors for anionic biomolecules such as amino acid, [15] peptides and proteins, [16] nucleotides andn ucleica cids [17] using ion pair interactions, we soughttodevise new ways for monitoring heparini nb iological fluids. We [17b,c] and others [18] have successfully used peptideb eacons for sensing.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Peptide Beacons for the Selective Ratiometric Detection of Heparin

Maity

Schmuck

2016

Chemistry A European J

Self Cite

View full text Add to dashboard Cite

Heparin is extensively used as an anticoagulant drug during surgery. Two fluorophore-functionalized cationic oligopeptides HS 1 and HS 2 were developed to monitor heparin ratiometrically in aqueous media. Upon binding to heparin, HS 1 and HS 2 undergo a conformational change from an open form to a folded form, which leads to a distinct change in the fluorescence properties. HS 1 switches from pyrene monomer emission to an excimer emission. For HS 2, a fluorescence resonance energy transfer (FRET) process is enabled between a naphthalene donor and a dansyl acceptor. This method is highly selective for heparin relative to other similar biological analytes such as hyaluronic acid or chondroitin sulfate. HS 1 and HS 2 could also detect heparin ratiometrically in diluted bovine serum. The strong ratiometric emission color change can also be observed by the naked eye. Addition of the polycationic protein protamine releases both HS 1 and HS 2 from their heparin complex, which simultaneously restores pyrene monomer emission for the first case and decreases the FRET process for the latter case, respectively. Dynamic light scattering (DLS) and AFM studies confirm aggregate formation of heparin with HS 1 and HS 2.

show abstract

“…The Schmuck group mostly focused on developing receptors and chemosensors for substrates of biological importance. [11] Amino acids, short peptides, and proteins were selected as potentialb indersf or CB [8] to be investigated by using aratiometric fluorescence-based technique.…”

mentioning

confidence: 99%

A Selective Cucurbit[8]uril‐Peptide Beacon Ensemble for the Ratiometric Fluorescence Detection of Peptides

Maity

Assaf

Sicking

et al. 2019

Chemistry A European J

Self Cite

View full text Add to dashboard Cite

A convenient supramolecular strategy for constructing a ratiometric fluorescent chemosensing ensemble, consisting of a macrocyclic host (cucurbit[8]uril CB[8]), and a pyrene‐tagged amphiphilic peptide beacon (AP 1), is reported. AP 1 unfolds upon encapsulation of the pyrene termini into the hydrophobic CB[8] cavity. This changes pyrene excimer to monomer emission. Substrates with higher affinity for the CB[8] cavity can displace AP 1 from the ensemble. The released AP 1 folds again to form a pyrene excimer, which allows for the ratiometric fluorescence monitoring of the substrate. In this report, the ensemble capacity for ratiometric fluorescence monitoring of biological substrates, such as amino acid derivatives, specific peptides, and proteins, in aqueous media is demonstrated.

show abstract

Reversible and Noncompetitive Inhibition of β‐Tryptase by Protein Surface Binding of Tetravalent Peptide Ligands Identified from a Combinatorial Split–Mix Library

Cited by 27 publications

References 50 publications

Miniature magnetic resonance system for point-of-care diagnostics

Miniature magnetic resonance system for point-of-care diagnostics

Fluorescent Peptide Beacons for the Selective Ratiometric Detection of Heparin

A Selective Cucurbit[8]uril‐Peptide Beacon Ensemble for the Ratiometric Fluorescence Detection of Peptides

Contact Info

Product

Resources

About