Reversibility of the haemodynamic effects of anabolic steroids in rats

Pesola, Maija K.

doi:10.1007/bf00636615

Cited by 26 publications

(17 citation statements)

References 22 publications

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“…CS NE CE NS in sedentary and exercised rats (14,15). We demonstrated that exercised animals exhibited a greater (two-way ANOVA, P < 0.05) accumulation of HSP72 in the cardiac muscle compared to sedentary animals (mean ± SEM; 677.16 ± 129.14 vs 246.24 ± 46.30 relative unit, respectively), independently of the nandrolone administration.…”

Section: Hsp73 Hsp72mentioning

confidence: 66%

Anabolic steroid- and exercise-induced cardiac stress protein (HSP72) in the rat

Lunz

Oliveira

Neves

et al. 2006

Braz J Med Biol Res

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The present study investigated the effects of exercise and anabolicandrogenic steroids on cardiac HSP72 expression. Male Wistar rats were divided into experimental groups: nandrolone exercise (NE, N = 6), control exercise (CE, N = 6), nandrolone sedentary (NS, N = 6), and control sedentary (CS, N = 6). Animals in the NE and NS groups received a weekly intramuscular injection (6.5 mg/kg of body weight) of nandrolone decanoate, while those in the CS and CE groups received mineral oil as vehicle. Animals in the NE and CE groups were submitted to a progressive running program on a treadmill, for 8 weeks. Fragments of the left ventricle were collected at sacrifice and the relative immunoblot contents of HSP72 were determined. Heart weight to body weight ratio was higher in exercised than in sedentary animals (P < 0.05, 4.65 ± 0.38 vs 4.20 ± 0.47 mg/g, respectively), independently of nandrolone, and in nandrolone-treated than untreated animals (P < 0.05, 4.68 ± 0.47 vs 4.18 ± 0.32 mg/g, respectively), independently of exercise. Cardiac HSP72 accumulation was higher in exercised than in sedentary animals (P < 0.05, 677.16 ± 129.14 vs 246.24 ± 46.30 relative unit, respectively), independently of nandrolone, but not different between nandrolone-treated and untreated animals (P > 0.05, 560.88 ± 127.53 vs 362.52 ± 95.97 relative unit, respectively) independently of exercise. Exercise-induced HSP72 expression was not affected by nandrolone. These levels of HSP72 expression in response to nandrolone administration suggest either a low intracellular stress or a possible less protection to the myocardium.

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Section: Hsp73 Hsp72mentioning

confidence: 66%

Anabolic steroid- and exercise-induced cardiac stress protein (HSP72) in the rat

Lunz

Oliveira

Neves

et al. 2006

Braz J Med Biol Res

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“…In the studies where baseline mechanical function of the hearts was compromised, the doses of different AAS were somewhat higher [27,50]. In particular, while…”

Section: Discussionmentioning

confidence: 99%

Ischemia/reperfusion injury is increased and cardioprotection by a postconditioning protocol is lost as cardiac hypertrophy develops in nandrolone treated rats

et al. 2010

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We hypothesized that Nandrolone (ND)-abuse induces cardiac hypertrophy, increases myocardial susceptibility to ischemia/reperfusion (I/R) injury, and reduces responsiveness to postconditioning (PostC) cardioprotection. Wistar-rats were ND-treated for 2-weeks (short_ND) or 10-weeks (long_ND). Vehicle-treated rats served as controls. Hearts were retrogradely perfused and left ventricular pressure (LVP) was measured before and after 30-min global ischemia. In subgroups of hearts, to induce cardioprotection a PostC protocol (five cycles of 10-s reperfusion and 10-s ischemia) was performed. β-adrenoreceptors, kinases (Akt and GSk-3β) and phosphatases (PP2A sub A and PP2A sub B) were examined by Western blot before and after ischemia. After 120-min reperfusion infarct-size was measured. Short_ND slightly increased cardiac/body weight ratio, but did not affect cardiac baseline nor post-ischemic contractile function or infarct-size when compared to vehicle hearts. However, PostC limited cardiac dysfunction much more in short_ND hearts than other groups. Although cardiac/body weight ratio markedly increased after long_ND, baseline LVP was not affected. Yet, post-ischemic contracture and infarct-size were exacerbated and PostC was unable to reduce infarct-size and ventricular dysfunction. While short_ND increased phosphatases, non-phosphorylated and phosphorylated Akt, long_ND reduced phosphatase-expression and Akt-phosphorylation. Both short_ND and long_ND had no effect on the GSK-3β-phosphorylation but increased the expression of β 2 -adrenoreceptors. In reperfusion, PostC increased Akt-phosphorylation regardless of protective effects, but reduced phosphataseexpression in protected hearts only. In conclusion: short_ND improves post-ischemic myocardial performance in postconditioned hearts. However, long_ND increases myocardial susceptibility to I/R injury and abolishes cardioprotection by PostC. This increased susceptibility might be related to steroid-induced hypertrophy and/or to altered enzyme expression/phosphorylation.

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“…In in vivo experiments ND treatment altered the inotropic and chronotropic left ventricular response as well as the vascular responses to isoproterenol [9]. These apparent contrasting findings ( [7] versus [8,9]) can find a reason on the intricate interplay existing among pre-load, post-load, heart geometry and chronotropic effects over inotropic response of the heart [10]. Moreover, the ventricular stiffness and, thus, the response to stretch of hypertrophic hearts is altered [7].…”

Section: Introductionmentioning

confidence: 98%

“…However, the same chronic treatment produced hypertrophy and a decrease in myocardial contractile reserve to ␤-adrenoceptor stimulation [8]. In in vivo experiments ND treatment altered the inotropic and chronotropic left ventricular response as well as the vascular responses to isoproterenol [9]. These apparent contrasting findings ( [7] versus [8,9]) can find a reason on the intricate interplay existing among pre-load, post-load, heart geometry and chronotropic effects over inotropic response of the heart [10].…”

Section: Introductionmentioning

confidence: 99%

Nandrolone-pretreatment enhances cardiac β2-adrenoceptor expression and reverses heart contractile down-regulation in the post-stress period of acute-stressed rats

Penna¹,

Abbadessa²,

Mancardi³

et al. 2007

The Journal of Steroid Biochemistry and Molecular Biology

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Reversibility of the haemodynamic effects of anabolic steroids in rats

Cited by 26 publications

References 22 publications

Anabolic steroid- and exercise-induced cardiac stress protein (HSP72) in the rat

Anabolic steroid- and exercise-induced cardiac stress protein (HSP72) in the rat

Ischemia/reperfusion injury is increased and cardioprotection by a postconditioning protocol is lost as cardiac hypertrophy develops in nandrolone treated rats

Nandrolone-pretreatment enhances cardiac β2-adrenoceptor expression and reverses heart contractile down-regulation in the post-stress period of acute-stressed rats

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