Reversed-phase porous silica rods, an alternative approach to high-performance liquid chromatographic separation using the sequential injection chromatography technique

Šatı́nský, Dalibor; Huclová, Jitka; Solich, Petr; Karlíček, R.

doi:10.1016/s0021-9673(03)01239-1

Cited by 58 publications

(2 citation statements)

References 14 publications

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“…The combination of nortriptyline and fluphenazine is compounds in pharmaceutical dosage forms or biological samples, such as sildenafil, [37] lamivudine, [38] parabenes and triamcinolone in cream formulation, [39] ecstasy, [40] fenfluramine, temazepam, oxazepam, and tamoxifen, [41] mianserin, [42] cephalosporin antibiotics in pharmaceuticals and body fluids, [43] p-amino salicylic acid, [44] corticosteroids in tablets, [45] glimepirid and glibenclamid, [46] acyclovir, [47] rifampicin, [48] tetracyclines, [49] levetiracetam, [50,51] and famotidine and fluoroquinolones. [52] The aim of this study is to establish a fast HPLC-method for simultaneous determination of nortriptyline and fluphenazine depending on the advantage of monolithic stationary phase, which can be used at high flow rates.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous HPLC-Determination of Nortriptyline and Fluphenazine in One Minute Using Monolithic Stationary Phase

Hashem

Jira

2013

Journal of Liquid Chromatography & Related Technologies

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& A simple, fast, robust, and accurate high-performance liquid chromatography (HPLC) method is described for simultaneous quantification of nortriptyline and fluphenazine in bulk powder and dosage forms. The chromatographic separation was carried out in less than one minute on Chromolith 1 Performance RP-18e column, which consists of monolithic rods of highly porous silica, using isocratic binary mobile phase of MeOH and 25 mM KH 2 PO 4 pH 4.5 in the ratio of 70: 30 at 5 mL min À1 flow rate and 40 C. The high porosity of stationary phase enables it to be used at high flow rates without problems concerning backpressure, these high flow rates in turn lead to strong reduction of analysis time. A diode array detector was used at 254 nm for detection. The method was validated for system suitability, linearity, precision, limits of detection and quantitation, specificity, and robustness. LOD were found to be 0.40 and 0.78 lg mL À1 for nortriptyline and fluphenazine, respectively. LOQ was 3.125 lg mL À1 for both analytes. Recovery values of this method were 98.50 and 98.00% for nortriptyline and fluphenazine, respectively, and reproducibility was within 2.36%. Robustness study was done for small changes in KH 2 PO 4 concentration and pH, temperature, flow rate, wavelength of detection, % of MeOH in mobile phase, and injection volume.

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Section: Introductionmentioning

confidence: 99%

Simultaneous HPLC-Determination of Nortriptyline and Fluphenazine in One Minute Using Monolithic Stationary Phase

Hashem

Jira

2013

Journal of Liquid Chromatography & Related Technologies

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“…Our innovative feature was that new hyphenated technique for analysis has been created coupling of short monolithic column with SIA manifold bringing a new dimension into SIA technique. The on-line coupling of the SIA technique with chromatographic monolithic column has been introduced in our previous works [5][6][7][8] and sequen-0731-7085/$ -see front matter © 2005 Elsevier B.V. All rights reserved. doi:10.1016/j.jpba.2005.07.032 tial injection chromatography system (SIC) has been used for the determination of salicylic acid, methylsalicylate, methylparaben, propylparaben, paracetamol, caffeine, benzoic acid, sodium diclofenac and other compounds in different pharmaceutical preparations.…”

Section: Introductionmentioning

confidence: 99%