Determination of ambroxol hydrochloride, methylparaben and benzoic acid in pharmaceutical preparations based on sequential injection technique coupled with monolithic column

Šatı́nský, Dalibor; Huclová, Jitka; Ferreira, Raquel L.C.; Montenegro, M.C.B.S.M.; Solich, Petr

doi:10.1016/j.jpba.2005.07.032

Cited by 57 publications

(9 citation statements)

References 20 publications

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“…The incorporation of a monolithic column into a SIA system or SIC system with spectrophotometry has been introduced for online sample pretreatment. A monolithic column was utilized for separation and determination of some pharmaceutical components including ambroxol hydrochloride, methylparaben, and benzoic acid [89]. …”

Section: Flow-based Optical Detectionmentioning

confidence: 99%

Recent Electrochemical and Optical Sensors in Flow-Based Analysis

Chailapakul

Ngamukot

Yoosamran

et al. 2006

Sensors

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Section: Flow-based Optical Detectionmentioning

confidence: 99%

Recent Electrochemical and Optical Sensors in Flow-Based Analysis

Chailapakul

Ngamukot

Yoosamran

et al. 2006

Sensors

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“…Various other methods such as HPLC [10–13], GLC [14, 15], the sequential injection technique coupled with a monolithic column [16], LC-MS [17], capillary electrophoretic [18] and fluorescence detection [19] are also reported for its determination from biological fluids. A few of the degradants and other impurities of ambroxol reported in the British Pharmacopiea [5] are shown in Fig.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification, and Characterization of One Degradation Product in Ambroxol by HPLC-Hyphenated Techniques

2014

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This study details the isolation, identification, and characterization of ambroxol’s unknown impurity. One unknown impurity of ambroxol was formed in the formulated drug under stress conditions [40°C /75% relative humidity (RH) for 6 months] with the relative retention time (RRT) 0.68 in RP-HPLC. The impurity was enriched by exposing it to heat and it was isolated by using preparative HPLC. The enriched impurity was purified and characterized using the following sophisticated techniques: 2D NMR (gDQ-COSY, gHSQC, and gHMBC), FTIR, and LC-MS/MS. On the basis of the spectral data, the impurity was characterized as trans-4-(6,8-dibromoquinazolin-3(4H)-yl)cyclohexanol.

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“…Only limited analytical methods were reported in the literature for Ambroxol. [2–10] The aim of this work was to develop and validate a simple, fast, and reliable isocratic reversed phase high-performance liquid chromatographic (HPLC) and ultraviolet (UV) methods for the determination of AMH in pharmaceutical dosage forms. The important features and novelty of the proposed method included sample treatment with sonication of small amount of powder sample at ambient temperature, centrifugation, dilution; short elution time with internal standard eluted prior to AMH; and good precision (relative standard deviation Relative standard deviation [RSD] <5%) and high recovery (>95%).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an high-performance liquid chromatographic, and a ultraviolet spectrophotometric method for determination of Ambroxol hydrochloride in pharmaceutical preparations

Muralidharan¹,

Kumar²,

Dhanara³

2013

J Adv Pharm Technol Res

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A high-performance liquid chromatographic (HPLC) and ultraviolet (UV) methods were developed and validated for the quantitative determination of Ambroxol hydrochloride (AMH) in pharmaceutical dosage form. HPLC was carried out by reversed phase (RP) technique on an RP-18 column with a mobile phase composed of acetonitrile and water (pH 3.5 adjusted with orthophosphoric acid [60:40, v/v]). UV method was performed with the λmax at 250 nm. Both the methods showed good linearity, reproducibility, and precision. No spectral or chromatographic interferences from the tablet excipients were found in UV and HPLC. The method was successfully applied to commercial tablets. Validation parameters such as linearity, precision, accuracy, and specificity were determined. The HPLC Limit of detection (LOD) and Limit of quantification (LOQ) for Ambroxol were found to be 1 and 5 ng/ml, respectively. The UV LOD and LOQ for Ambroxol were found to be 1 and 4 μg/ml, respectively. The results were statistically compared using one-way analysis of variance. The proposed economical method could be applicable for routine analysis of AMH and monitoring of the quality of marketed drugs.

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Determination of ambroxol hydrochloride, methylparaben and benzoic acid in pharmaceutical preparations based on sequential injection technique coupled with monolithic column

Cited by 57 publications

References 20 publications

Recent Electrochemical and Optical Sensors in Flow-Based Analysis

Recent Electrochemical and Optical Sensors in Flow-Based Analysis

Isolation, Identification, and Characterization of One Degradation Product in Ambroxol by HPLC-Hyphenated Techniques

Development and validation of an high-performance liquid chromatographic, and a ultraviolet spectrophotometric method for determination of Ambroxol hydrochloride in pharmaceutical preparations

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