Reversed‐phase monoliths prepared by UV polymerization of divinylbenzene

Abstract

Many different techniques have been developed to prepare monolithic materials specifically for chromatographic techniques. The two most popular polymerization techniques being thermal or via ultra violet (UV) light. Whereas thermal polymerization is easily employed for a whole variety of monomer and porogen systems, UV polymerization has been limited to methacrylate-based systems, and styrenic systems have been avoided due to their strong absorbance at low wavelengths. By careful consideration of wavelength, initiator and other system components, it was proven that reversed-phase columns for the separation of proteins and peptides can be prepared using divinylbenzene through UV initiation of 2-methyl-4'-(methylthio)-2-morpholinopropiophenone at a wavelength of 350 nm.

Highly ordered monolithic structures by directional freezing and UV-initiated cryopolymerisation. Evaluation as stationary phases in high performance liquid chromatography

“…However, as Flook et al reported the preparation of polymer monoliths by photopolymerisation within COC tubing is not a trivial task and the right photo-initiator and wavelength used should be carefully chosen. 28 In this study, two different methods were used for the surface modication of COC tubing. The rst method consisted in the chemical modication of the COC tubing by hydrogen abstraction using a similar approach to that reported by Flook et al 28 For this, the COC tubing was lled with a 3 wt% solution of MTMPP in t-butanol : water (75 : 25 v/v) and then irradiated at 260 nm.…”

“…28 In this study, two different methods were used for the surface modication of COC tubing. The rst method consisted in the chemical modication of the COC tubing by hydrogen abstraction using a similar approach to that reported by Flook et al 28 For this, the COC tubing was lled with a 3 wt% solution of MTMPP in t-butanol : water (75 : 25 v/v) and then irradiated at 260 nm. These modied tubes were then used as 'moulds' for the directional freezing and photo-polymerisation reactions.…”

Organic monoliths for high‐performance reversed‐phase liquid chromatography

“…Flook et al reported monoliths prepared from DVB by optimizing the UV irradiation. The column material and porogens were evaluated in this work.…”

Fabrication of acrylate monolith using photopolymerization: Effect of light intensity on electrochromatographic performance

“…This polymerization process can be initiated thermally or by UV irradiation via the addition of an initiator in the mixture . The main advantages of photopolymerization are the fast and easy preparation of the porous materials and the possibility to localize the monolith inside the separation area .…”