Reversed-phase liquid chromatographic studies of non-ionic surfactants and related solutes

A reversed phase HPLC assay for the simultaneous quantitation of non-ionic and ionic surfactants in bioprocess intermediates

“…Pardue and Williams demonstrated adequate recovery and yield using ion exchange resins in the absence of ion-pairing agents [10]. A reversed phase quantitation of Triton, using an CH 3 CN and water gradient, has been described by Varughese et al using an octadecyl silane column with a refractive index detector to evaluate the effect of increasing methylene groups on the retention times of various non-ionic surfactants [11]. Using a silica column and aqueous solvents, Chen et al were able to quantitate levels of cationic surfactants present in household cosmetics as well as in some pharmaceutical formulations [12].…”

Molecular Weight and EON Distribution of Industrial Polyethoxylated Surfactants by High Performance Size Exclusion Chromatography

“…Octadecyl-or octyl-sylane columns were used for such separations (5 -9). Silica gel (6, 7, 10 -12) and silica with chemically bonded nitrile (6,7), diol (7), and amino phases (7 -9,13 -18) were also tested as column packing in normal-phase HPLC separation. However, the main problem in the analysis of mixtures of oligomers by HPLC is the effective fractionation of chains with widely different affinities.…”