Reversed-phase high-pressure liquid chromatography of spiramycin

Mourot, D.; Delépine, B.; Boisseau, J.; Gayot, G.

doi:10.1016/s0021-9673(01)85258-4

Cited by 18 publications

(2 citation statements)

References 3 publications

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“…12). By using peak height ratios (drug to internal standard) in the calculations, the coefficient of variation for the assays decreased slightly, but by combining it with the data-pair technique [5] the coefficients of variation decreased by a factor of three, down to 1.5%. Other macrolides such as Tylosins, Turimycins and 9-Propionylmaridomycins were chromatographed using the eluent system for Spiramycins, but only the Tylosins showed satisfactory separation (Fig.…”

Section: Resultsmentioning

confidence: 99%

High performance thin‐layer chromatography of some sixteen‐membered ring macrolide antibiotics

Bens

Bossche

Moerloose

1980

J. High Resol. Chromatogr.

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SummaryThe use of HPTLC in the analysis of some sixteen-membered ring macrolide antibiotices was examined. In the case of Spiramycins, instrumentalized HPTLC proved to be very efficient for the separation and determination of these antibiotics. With the use of an internal standard together with the datapair technique in sampling and evaluation of the HPTLC plates, a coefficient of variation less than 1.5% could be achieved when determining the different Spiramycins. Other sixteenmembered macrolides, such as Tylosins, Turimycins and 9-Propionylmaridomycins can be separated with sufficient resolution for quantitative work, in spite of their extremely simular structures and large molecular weights. Detection is always at wavelengths which agree with the intrinsic absorption maximum of the chromophors of the components (e. g. 282 nm for Tylosins, 232 nm for Spiramycins and Turimycins, 195 nm for 9-Propionylmaridomycins).

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Section: Resultsmentioning

confidence: 99%

High performance thin‐layer chromatography of some sixteen‐membered ring macrolide antibiotics

Bens

Bossche

Moerloose

1980

J. High Resol. Chromatogr.

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“…Determination of spiramycin by HPLC was performed on LiChrosorb CP Spher C18 (25 cm by 4.6 mm [inside diameter]) with a solvent of 4 g of H3PO4-0.5 g of tetramethylammonium chloride in 500 ml of water mixed with 500 ml of acetonitrile with UV detection at 233 nm and operation at a temperature of 23°C (27). The quantitation level of the HPLC was 0.1 ,ug/ml.…”

Section: Materuils and Methodsmentioning

confidence: 99%

Pharmacokinetics of spiramycin in the rhesus monkey: transplacental passage and distribution in tissue in the fetus

Ven

Galama

Camps

et al. 1994

Antimicrob Agents Chemother

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Transplacental transfer of spiramycin was investigated in a rhesus monkey model to study whether the antibiotic reaches therapeutic levels in the fetus. Spiramycin concentrations were measured by bioassay and high-performance liquid chromatography. Pharmacokinetic parameters were determined for bioactive spiramycin as measured by the bioassay. Pharmacokinetic pilot studies showed that spiramycin distribution follows a two-compartment model in rhesus monkeys. Following a single intravenous dose of 50 or 250 mg, dose-dependent kinetics were observed. At a dose of 50 mg, 10Wo of the dose was excreted unchanged in the urine. At the higher dose of 250 mg, an oliguric effect was observed. Spiramycin concentrations in fetal serum were measured over time while the maternal concentration was maintained at a constant level. During a 5-h experiment, a maximum fetal-maternal serum ratio of 0.27 was found. In three fetuses, concentrations in serum and tissue were measured following intravenous administration of 50 mg of spiramycin twice daily to the mother for at least 7 days. The fetal-maternal serum ratios were found to be 0.4 to 0.58 after intravenous administration of the final dose of 50 mg to the mother. It appeared that spiramycin accumulated in the soft tissues, especially in the liver and spleen, of both the mother and the fetus. The concentration in placental tissue appeared to be 10 to 20 times that of the concentration in fetal serum. The concentration of spiramycin in amniotic fluid was about five times higher than the concentration in fetal serum. Another important observation was that absolutely no spiramycin was found in the brain.

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