Analysis of spiramycin by capillary electrophoresis

González-Hernández, Rolando; Li, Yongmin; Schepdael, Ann Van; Roets, Eugène; Hoogmartens, Jos

doi:10.1002/(sici)1522-2683(19990801)20:12<2407::aid-elps2407>3.0.co;2-o

Cited by 13 publications

(3 citation statements)

References 12 publications

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“…Another approach to the analysis of spiramycin is the utilization of a mixed micellar system [22]. Under the optimized buffer conditions of 20 mM sodium cholate and 12 mM CTAB in an 80 mM Na 2 HPO 4 buffer, pH 7.5, it was possible to separate spiramycins I, II, III and IV and neospiramycins I, II and III [22].…”

Section: Macrolidesmentioning

confidence: 99%

“…Under the optimized buffer conditions of 20 mM sodium cholate and 12 mM CTAB in an 80 mM Na 2 HPO 4 buffer, pH 7.5, it was possible to separate spiramycins I, II, III and IV and neospiramycins I, II and III [22]. Linearity was demonstrated for spiramycin I from 0.3 to 0.7 mg/mL, and limits of 0.08% (LOQ) and 0.025% (LOD), relative to a 2.0 mg/mL solution, were reported [22]. Mixed micellar systems were also used for the determination of tylosin, erythromycin and their related substances [23].…”

Section: Macrolidesmentioning

confidence: 99%

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Analysis of antibiotics by capillary electrophoresis

Flurer

2001

Electrophoresis

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This article reviews recent developments in the characterization of antibiotics. Many capillary electrophoretic techniques have been utilized in their analyses, addressing various aspects of quantifying, profiling and monitoring. Sensitive electrochemical and laser-induced fluorescence detection systems have been utilized, demonstrating trace level determinations in clinical settings and in environmental samples. Different sample introduction methods have been explored, enhancing detection sensitivity, or reducing or eliminating sample manipulation prior to injection.

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Section: Macrolidesmentioning

confidence: 99%

Section: Macrolidesmentioning

confidence: 99%

Analysis of antibiotics by capillary electrophoresis

Flurer

2001

Electrophoresis

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“…Although mixed micelle systems, which are composed of two or three surfactants are not commonly used, it has been found that they have unique selectivity in some cases [14][15][16][17][18][19][20][21][22]. As CE has the benefit of high efficiency and versatile selectivity, it has been found to be useful in the separation of polypeptide antibiotics, which have a very complex composition [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Analysis of bacitracin by micellar electrokinetic capillary chromatography with mixed micelle in acidic solution

et al. 2001

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A method used for quantitative analysis of bacitracin with micellar electrokinetic capillary chromatography (MEKC) is described. As capillary zone electrophoresis gave poor separation selectivity, MEKC was preferable. It was found that a zwitterionic surfactant, 3-(N,N-dimethylhexadecylammonium)-propanesulfonate (PAPS) gave the best selectivity among the several surfactants studied. As the analytes tend to adsorb onto the capillary wall due to their positive charge, an acidic solution composed of Tris-phosphate buffer at pH 2.5 was necessary to diminish such adsorption. The peak tailing caused by relatively strong ion pair interaction between the analyte and PAPS micelle could be reduced by adding nonionic surfactant Brij 35 to the PAPS solution. This phenomenon is possibly explained by a mixed micelle mechanism. In order to obtain the optimal conditions and to test the method robustness, a central composite experimental design was performed. The optimal conditions are as follows: 44 cm length of fused-silica capillary with 50 microm inner diameter, 90 mM Tris-phosphate buffer (pH 2.5) containing 17 mM PAPS and 0.3% w/v-Brij 35, 18 kV applied voltage, UV detection at 192 nm and 25 degrees C column temperature. Under the optimal conditions, more than 50 peaks could be obtained in 30 min. The method had a linearity range from 1 to 0.05 mg/mL (concentration of bacitracin A). The limit of quantitation (LOQ) and limit of detection (LOD) were 0.005 and 0.0012 mg/mL, respectively.

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Simultaneous determination of metronidazole and spiramycin in bulk powder and in tablets using different spectrophotometric techniques

Khattab¹,

Ramadan²,

Hegazy³

et al. 2010

Drug Testing and Analysis

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Metronidazole (MZ) is an anti-infective drug used in the treatment of anaerobic bacterial and protozoa infections in humans. It is also used as a veterinary antiparasitic drug. Spiramycin (SP) is a medium-spectrum antibiotic with high effectiveness against Gram-positive bacteria. Three simple, sensitive, selective and precise spectrophotometric methods were developed and validated for the simultaneous determination of MZ and SP in their pure form and in pharmaceutical formulations. In methods A and B, MZ was determined by the application of direct spectrophotometry and by measuring its zero-order (D(0)) absorption spectra at its λ(max) = 311 nm. In method A, SP was determined by the application of first derivative spectrophotometry (D(1)) and by measuring the amplitude at 218.3 nm. In method B, the first derivative of the ratio spectra (DD(1)) was applied, and SP was determined by measuring the peak amplitude at 245.6 nm. Method C entailed mean centering of the ratio spectra (MCR), which allows the determination of both MZ and SP. The methods developed were used for the determination of MZ and SP over a concentration range of 5-25 µg ml(-1). The proposed methods were used to determine both drugs in their pure, powdered forms with mean percentage recoveries of 100.16 ± 0.73 for MZ in methods A and B, 101.10 ± 0.90 in method C, 100.09 ± 0.70, 100.02 ± 0.88 and 100.49 ± 1.26 for SP in methods A, B and C, respectively. The proposed methods were proved using laboratory-prepared mixtures of the two drugs and were successfully applied to the analysis of MZ and SP in tablet formulation without any interference from each other or from the excipients. The results obtained by applying the proposed methods were compared statistically with a reported HPLC method and no significant difference was observed between these methods regarding both accuracy and precision.

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Analysis of spiramycin by capillary electrophoresis

Cited by 13 publications

References 12 publications

Analysis of antibiotics by capillary electrophoresis

Analysis of antibiotics by capillary electrophoresis

Analysis of bacitracin by micellar electrokinetic capillary chromatography with mixed micelle in acidic solution

Simultaneous determination of metronidazole and spiramycin in bulk powder and in tablets using different spectrophotometric techniques

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