Reversed-phase high-performance liquid chromatography versus spectrophotometric assay for thimerosal in Cuban recombinant hepatitis B vaccine

Simultaneous determination of thimerosal and aluminum in vaccines and pharmaceuticals with the use of HPLC method

“…On the other hand, literature data clearly indicate that cation RHg + reacts with DT to form chelates with 1:1 ratio [51,54]. TM, as the compound with polarized S-Hg bond, C 2 H 5 -Hg δ+ -δ− S-C 6 H 4 -COO − Na + , undergoes cleavage in solution induced by, e.g., light or temperature [7,10,[36][37][38][39]. Consequently, thiosalicylate anion is labile [8] and can be exchanged by stronger nucleophile, e.g., DT ligand.…”

“…Determination of thimerosal in pharmaceuticals is carried out in two ways: as total Hg [26][27][28][29][30][31][32][33][34] or directly as thimerosal [35][36][37][38][39][40][41][42][43][44]. The total Hg determination methods require mineralization and atomization of sample to form mercury vapor.…”

“…However, there are also a few methods where there is no need to apply volatile mercury generation since intact TM is determined. Examples include high-performance liquid chromatography (HPLC) [7,10,[35][36][37][38][39][40] and a few electrochemical methods [42] as well as ultraviolet-visible (UV-vis) spectrometry [43,44]. HPLC determination of TM in pharmaceuticals can be realized with spectrophotometric [7,8,[36][37][38], electrochemical [39,40], or inductively coupled plasma mass spectrometry detection [45,46].…”

“…HPLC determination of TM in pharmaceuticals can be realized with spectrophotometric [7,8,[36][37][38], electrochemical [39,40], or inductively coupled plasma mass spectrometry detection [45,46]. The respective spectrophotometric detection is typically realized in UV range [7,[36][37][38]40].…”

Liquid chromatography coupled to molecular fluorescence with postcolumn UV sensitization for thimerosal and derivative compounds monitoring in environmental samples

“…The detection of THM at trace level in environmental samples is of crucial importance. In the past, various analytical techniques including HPLC with electrochemical detection, enzymatic amperometry, colorimetry, atomic absorption spectrometry, and atomic fluorescence spectrometry have been developed to detect THM and derivatives . As an alternative, mass spectrometry detectors are preferable in terms of sensitivity, accuracy, generated waste and others requirements of routine analysis, however, the equipment and running costs are prohibitive to many laboratories .…”