Reversed-phase high-performance liquid chromatography method with coulometric electrochemical and ultraviolet detection for the quantification of vitamins B1 (thiamine), B6 (pyridoxamine, pyridoxal and pyridoxine) and B12 in animal and plant foods

Lebiedzińska, Anna; Marszałł, Michał Piotr; Kuta, Jadwiga; Szefer, Piotr

doi:10.1016/j.chroma.2007.09.072

Cited by 93 publications

(52 citation statements)

References 39 publications

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“…Separation of B 6 vitamers is quite different from previously reported for vitamin B 12 derivatives (cobalamins) separations. In contrast to B 6 , cobalamins cannot be simply separated (Lebiedzińska et al 2007). As mentioned, based on DFT calculations, this may be attributed to the much larger size of B 12 in comparison to B 6 .…”

Section: Discussionmentioning

confidence: 91%

“…Separation of B 6 vitamer fragments is quite different from that previously reported for vitamin B 12 derivatives (cobalamins). In contrast to B 6 vitamers, cobalamins cannot be simply separated (Lebiedzińska et al 2007). Based on DFT calculations, this may be attributed to the much larger size of B 12 compared to B 6 , leading to an electrostatic potential distribution that is more disperse, where functional group modifications of the outer coordination sphere have relatively little effect on the electrostatic properties.…”

Section: Computational Modeling Using Density Functional Theorymentioning

confidence: 99%

“…Most separation and detection procedures are reversed-phase high-performance liquid chromatography (HPLC) techniques (Eitenmiller et al 2008) (Ciulu et al 2011;Jin et al 2012;Langer and Lodge 2014), and MS (Hälvin et al 2013;van der Ham et al 2012). Methods using electrochemical detection (ED) have also been proposed but applications to samples other than pharmaceuticals supplements and animal foods are few (Lebiedzińska et al 2007;Marszałł et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Detection of B6 Vitamers in Grain Products: Experimental and Computational Studies

et al. 2017

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A reversed phase high-performance liquid chromatography method (HPLC) with coulometric electrochemical detection has been applied and validated for the simultaneous analysis of pyridoxamine (PM), pyridoxal (PL), and pyridoxine (PN) in cereal products. Isocratic separation was achieved using a C 18 column with a mobile phase consisting of methanol-phosphate buffer (10:90) and 0.018 M trimethylamine adjusted to pH 3.55 with 85% orthophosphoric acid. The limits of detection for PM, PL, and PN were 0.28, 0.36, and 0.43 ng mL −1 , respectively, with vitamins recoveries ranging from 90.4 to 98.1%. The applied method for the analysis of B 6 vitamins naturally present in grain products, offers a simple and fast sample preparation without derivatization. To understand vitamer separation further, methods of computational chemistry were employed. Specifically, density functional theory (DFT) was used to determine electrostatic potentials, as well as the charges of each vitamer. A number of correlations were established between these properties and elution order.

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Section: Discussionmentioning

confidence: 91%

Section: Computational Modeling Using Density Functional Theorymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of B6 Vitamers in Grain Products: Experimental and Computational Studies

et al. 2017

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“…Heudi et al (2005) have reported liquid chromatography separation of nine water-soluble vitamins with LC-MS analysis in premixes. The HPLC procedures with the appropriate detection system are considered as the most convenient methods for the determination of WSV especially the B-group (Lebiedzinska et al, 2007). The HPLC coupled with electrochemical detector (HPLC-ED) is believed to be sensitive and selective method for the determination of redox compounds.…”

Section: Simultaneous Determination Of Water-soluble Vitaminsmentioning

confidence: 99%

“…It is one of the analytical methods that has been recommended for the determination of B-group vitamins in pharmaceutical formulations and biological matrices (Ekinci, 2005;Marszall et al, 2005;Mimica et al, 2002). For analysis of seafood (fish, mussels, oysters) and food products of plant origin (apple juices and fortified fruit juices), Lebiedzinska et al (2007) have reported an HPLC method with ED and UV detection for the simultaneous determination of vitamins B1, B6, and B12. The method offers high accuracy, better repeatability and reproducibility and a relative short time for quantification of the three water-soluble vitamins assayed in food samples.…”

Section: Simultaneous Determination Of Water-soluble Vitaminsmentioning

confidence: 99%

Simultaneous detection of water-soluble vitamins using the High Performance Liquid Chromatography (HPLC) - a review

Abano

Dadzie

2014

Croat. J. Food Sci. Technol.

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SummaryThe water-soluble vitamins (WSV): ascorbic acid (vitamin C), thiamine (B1), riboflavin (B2), niacin (B3), panthothenic acid (B5), pyridoxine, and pyridoxal (B6), folic acid (B9), biotin(B8) , and B12 are very essential in the diet of humankind. As a result of ever increasing pressures from both consumers and legal enforcers, to specify accurately nutritive compositions of WSV that are present in food materials, many researchers have attempted to fill this niche through the provision of highly sensitive and rapid high performance liquid chromatography (HPLC) procedures. In view of the health benefits of WSV, a replete of HPLC methods have been developed for simultaneous determination of their contents in nature and fortified food samples, nutritional supplements, as well as blood plasmas. The rate of losses of these vitamins during food processing and analysis, in addition to their transient dynamics, presents complexities in developing a highly sensitive HPLC procedure for their simultaneous separations and assays. This review critically assesses the different HPLC procedures developed by researchers and available in the open literature for simultaneous determination of water-soluble vitamins (WSV) in dried tropical fruits materials. The study revealed that not a single chromatographic run developed by researchers can simultaneously elute all the WSV at a time. However, the HPLC procedures that are capable of determining all the WSV were coupled with electrospray ionization mass spectroscopy (ESI-MS), thus making the set-up expensive.

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HPLC Techniques with Electrochemical Detection

Santamaría

Barambio

Arribas

et al. 2015

Agricultural and Food Electroanalysis

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Reversed-phase high-performance liquid chromatography method with coulometric electrochemical and ultraviolet detection for the quantification of vitamins B1 (thiamine), B6 (pyridoxamine, pyridoxal and pyridoxine) and B12 in animal and plant foods

Cited by 93 publications

References 39 publications

Detection of B6 Vitamers in Grain Products: Experimental and Computational Studies

Detection of B6 Vitamers in Grain Products: Experimental and Computational Studies

Simultaneous detection of water-soluble vitamins using the High Performance Liquid Chromatography (HPLC) - a review

HPLC Techniques with Electrochemical Detection

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