Reversed-Phase High-Performance Liquid Chromatography Analysis of β-Lactoglobulin and α-Lactalbumin in Different Types of Milk

Whey Proteins Characterization, Free Amino Acids Profile and Antimicrobial Study of Some Dairy Drinks based on Milk Serum

“…The elution order shows BSA and α -La eluted before βlactglobulins. For β-Lg B were eluted before β-Lg A [10,13,37]. In chromatogram of Figure 1b can be observed a good separation of each studied proteins and the small peaks that are attributable to the other minor protein present in Milk Serum sample.…”

“…Nowadays, a variety of methods have been developed to analyses whey proteins: reversedphase high performance liquid chromatography (RP-HPLC) [5,[8][9][10], ultra HPLC tandem mass spectrometry [9,[11][12][13][14][15], electrophoretic techniques [9,16,17], immunoaffinity chromatography( [18,19], Enzyme Linked Immunosorbent Assay (ELISA) [20] [21] and a novel visualized microarray method [22]. However, HPLC method allows rapid and automated analysis, characterized by good separations, high resolutions and accuracy and reproducible results, especially for simultaneous detection of multiple whey proteins.…”

Chromatographic profile of major whey proteins in some dairy beverages based on milk serum

“…The elution order ( Figure 1a ) shows that BSA and α-La protein were eluted before the β-lactoglobulins components (β-Lg A and B). Also, the β-Lg B protein was eluted before β-Lg A protein [ 11 , 14 , 35 ].…”

“…Recently, the researchers have developed a variety of methods to analyze whey proteins like as: reversed-phase high-performance liquid chromatography (RP-HPLC) [ 5 , 8 – 11 ], ultra-HPLC tandem mass spectrometry [ 10 , 12 – 16 ], electrophoretic techniques [ 10 , 17 , 18 ], immunoaffinity chromatography [ 19 , 20 ]. Enzyme-linked immunosorbent assay (ELISA) [ 21 , 22 ] and a novel visualized microarray method [ 23 ].…”

Fluorescence detection of milk allergen β-lactoglobulin based on aptamers and WS2 nanosheets

“…To date, many analytical methods have been developed to detect β-Lg. Traditional chromatography is commonly used for β-Lg monitoring, including high performance liquid chromatography (HPLC), 10 ultra-high performance liquid chromatography (UHPLC), 11 and liquid chromatography tandem mass spectrometry (LC-MS/MS). 12 In addition, some novel detection methods, such as enzyme-linked immunosorbent assay, 13 photoelectrochemical immunoassay, 14 lateral flow immunoassay, 15 real-time quantitative polymerase chain reaction, 16 transient isotachophoresis, 17 and molecular imprinting, 18 have also been used for the detection of β-Lg.…”