volume 65, issue 2, P215-232 2007
DOI: 10.1016/j.ejpb.2006.07.014
View full text

Abstract: The present work explores, using response surface methodology, the main and interaction effects of some process variables on the preparation of a reversed chitosan-alginate polyelectrolyte complex (PEC) with entrapped alpha-amylase for stability improvement. A 3(3) full factorial design was used to investigate the effect of the chitosan and alginate concentrations and hardening time on the percent entrapment, time required for 50% (T(50)) and 90% (T(90)) enzyme release, and particle size. The beads were prepar…

expand abstract