Reverse Transcriptase PCR Amplification of EWS/FLI-1 Fusion Transcripts as a Diagnostic Test for Peripheral Primitive Neuroectodermal Tumors of Childhood

Sorensen, Poul H.; Liu, Xian Fang; Delattre, Olivier; Rowland, Jon M.; Biggs, Christopher; Thomas, Gilles; Triche, Timothy J.

doi:10.1097/00019606-199303000-00022

Cited by 31 publications

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“…No EWS/ERG fusion transcripts were observed in tumor samples (data not shown). To confirm the PCR findings, amplified products of nested PCR were transferred onto nylon membranes and separately probed with 2 oligonucleotide probes, respectively, derived from EWS (Sorensen et al, 1993) and FLI-1 (Downing et al, 1993). All bands identified by nested PCR were specifically hybridized by these probes, and no bands were observed either in normal muscle or in negative controls (Table 1, Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Two identical membranes were separately hybridized with EWS (Sorensen et al, 1993) and FLI-1 (Downing et al, 1993) probes, respectively. Filters were washed with 2 ϫ SSC, 0.1% SDS and 0.2 ϫ SSC, 0.1% SDS at 65°C.…”

Section: Southern Blot Analysis Of Pcr Productsmentioning

confidence: 99%

“…GCT, giant-cell tumor; ABC, aneurysmal bone cyst; PVNS, pigmented villonodular synovitis.-2 According to the staging system of the Musculoskeletal Tumor Society(Sorensen et al, 1993).-3 I, type I; II, type II.-4 ND, not determined.…”

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Identification of EWS/FLI-1 transcripts in giant-cell tumor of bone

et al. 2000

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Section: Resultsmentioning

confidence: 99%

Section: Southern Blot Analysis Of Pcr Productsmentioning

confidence: 99%

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Identification of EWS/FLI-1 transcripts in giant-cell tumor of bone

et al. 2000

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“…Fli-1 protein expression detected by immunohistochemical stain is another useful method, with reported sensitivity of greater than 70% and specificity of more than 90% [8]. Cytogenetic and molecular genetic identification of the ES/PNET-associated translocation is the "gold standard" as virtually all extraskeletal ES/pPNETs are characterized by the translocation t(11:22)(q24;q12) (resulting in the fusion of the EWS gene on chromosome 22 to the Fli-1 gene on chromosome 11) or other variant translocations involving the EWS gene [3,9].…”

Section: Discussionmentioning

confidence: 99%

Primary Ewing sarcoma/peripheral primitive neuroectodermal tumor of the vulva

2008

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“…Ewing's sarcoma/pPNET belongs to a group of poorly differentiated small round-cell tumors, including alveolar rhabdomyosarcoma, neuroblastoma, and lymphoblastic lymphoma. 7 To distinguish between Ewing's sarcoma/pPNET and these other small round-cell tumors is sometimes difficult by conventional morphologic methods, especially if the tumor arises in an unusual region. About 7% of Ewing's sarcoma/pPNET occur in extraosseous soft tissue.…”

Section: Discussionmentioning

confidence: 99%

Ewing's sarcoma/peripheral primitive neuroectodermal tumor (pPNET) arising in the omentum as a multilocular cyst with intracystic hemorrhage

Tanida

Tanioka

Inukai

et al. 2000

Journal of Gastroenterology

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A rare case of Ewing's sarcoma/peripheral primitive neuroectodermal tumor arising in the greater omentum in a 41-year-old man is reported. The patient presented with a hemorrhagic mesenteric cyst that was disclosed by the results of an abdominal echogram, a computed tomography scan, and magnetic resonance imaging. A laparotomy showed a multilocular cyst with intra-cystic hemorrhage. Histologically, the tumor wall consisted of sheets of small round cells separated by thick desmoplastic stroma. Rosette formations or ribbon-like cell arrangements were absent. Further pathological examination revealed that the membrane of the tumor cells was positive for MIC-2, and negative for epithelial membrane antigen, cytokeratin, and desmin, which are usually positive in intra-abdominal desmoplastic small round-cell tumors. An EWS/FLI1 fused transcript was detected by reverse transcription-polymerase chain reaction. These findings confirmed the diagnosis of Ewing's sarcoma/peripheral primitive neuroectodermal tumor. The patient died of tumor recurrence 4 months after his first admission. The autopsied tumor tissue exhibited neural differentiation in certain regions. To our knowledge, this is the first case to be reported of Ewing's sarcoma/peripheral primitive neuroectodermal tumor arising in the omentum with unique pathological features and the occurrence of partial neural differentiation during the clinical course. This case pointed out to us, as gastroenterologists, that only thorough examination confirms a definitive diagnosis of small round-cell tumor of the abdomen, it also shows that Ewing's sarcoma/peripheral primitive neuroectodermal tumor should be included in the differential diagnosis of cystic lesions in the omentum.

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Reverse Transcriptase PCR Amplification of EWS/FLI-1 Fusion Transcripts as a Diagnostic Test for Peripheral Primitive Neuroectodermal Tumors of Childhood

Cited by 31 publications

References 0 publications

Identification of EWS/FLI-1 transcripts in giant-cell tumor of bone

Identification of EWS/FLI-1 transcripts in giant-cell tumor of bone

Primary Ewing sarcoma/peripheral primitive neuroectodermal tumor of the vulva

Ewing's sarcoma/peripheral primitive neuroectodermal tumor (pPNET) arising in the omentum as a multilocular cyst with intracystic hemorrhage

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