Reverse phase high speed liquid chromatography of antibiotics.

Alkalimetric microassay of cephalosporins.

“…Direct hydrolysis with lactamase (U), 2) direct hydrolysis with esterase (E), and 3) lactamase-catalyzed hydrolysis of the deacetylated solutions (L2) from the preceding reaction (2). As is apparent from the results in Table 3 the stoichiometric relation 2E=2L2=L1 holds within 1 % for the purified materials.…”

Separation of Penicillin and Its Major Degradation Products by Ion-Pair Reversed-Phase High-Pressure Liquid Chromatography

“…However, the availability of small-particle size packings for reversed-phase chromatography suggests that very high chromatographic efficiencies and shorter analysis times can be expected with this procedure (8,9). The most recent papers deal with anion-exchange chromatography (5), reversed-phase chromatography (61, and NMR spectroscopy (7).…”

An HPLC procedure for monitoring penicillin G fermentations

“…High performance liquid chromatography (HPLC) has become the method of choice for the quantitative analysis of penicillins. The application of HPLC to the separation and determination of penicillin antibiotics was first achieved using ion-exchange stationary phases (Tsuji and Robertson, 1975) and later by reversed-phase columns (White et aL, 1977). Most of these reported HPLC procedures have been applied to the analyses of commercial antibiotics (Hoogmartens et aL, 1982) pharmaceutical preparations (Athanikar et al, 1983) and penicillins in body fluids (Nilsson-Ehle, 1983).…”