Reversal of Multidrug Resistance of KBV200 Cells by Triterpenoids Isolated fromPoria cocos

Abstract

Multidrug resistance (MDR) of tumor cells is one of the major problems encountered during cancer chemotherapy. In this paper, we isolated eight triterpenoids from Poria cocos and evaluated their effects on reversing MDR of KBV200 cells. Eight triterpenoids increase significantly vincristine-induced cytotoxicity in drug-resistant KBV200 cells at the concentrations of 12.5 µg/mL and 25 µg/mL. Dehydrotumulosic acid showed the best reversal effect: it increased KBV200 apoptosis induced by vincristine and inhibited P-gp function through enhancing the accumulation and retention of fluorescent P-gp substrate rhodamine 123 in KBV200 cells but had no effect on P-gp expression.

The inhibition of Mpro, the primary protease of COVID-19, byPoria cocosand its active compounds: a network pharmacology and molecular docking study

“…50 Dehydrotumulosic acid has anti-inammatory activity independent of glucocorticoid mechanism, and it can play an anti-tumor role by inhibiting the efflux of anticancer drugs, thus reversing cell resistance. 51,52 Trametenolic acid also has anti-tumor effect, which plays a role by anti-proliferation and reversing cell drug resistance. 53,54 kB pathway, 68 and other studies have shown that stellasterol has potential as a hypoglycemic agent.…”

Redox-responsive F127-folate/F127-disulfide bond-D-α-tocopheryl polyethylene glycol 1000 succinate/P123 mixed micelles loaded with paclitaxel for the reversal of multidrug resistance in tumors

“…Cells were treated with DCFH-DA for 30 min, washed three times with ice-cold PBS, and then harvested and resuspended in PBS. 36 The flow cytometry was used to measure the fluorescence of each sample (FACSCalibur; BD Biosciences, San Jose, CA, USA).…”

Current Advancements in Antitumor Properties and Mechanisms of Medicinal Components in Edible Mushrooms

“…Chiu et al reported that a fungal protein from Ganoderma microsporum suppressed the growth of MDR subline by promoting Akt/mTOR signaling pathway mediated apoptosis and autophagy [81]. Moreover, incubation of drug-resistant KBV200 cells with eight triterpenoids from Poria cocos induced vincristine-induced apoptosis [82]. Interestingly, a combined administration of pachymic acid and dehydrotumulosic acid purified from Poria cocos with doxorubicin (DOX) can enhance the sensitivity of drug-resistant MCF cells via inhibition of P-glycoprotein (P-gp)-mediated MDR [83] (Table 2).…”