Reversal of copper(II)-induced methemoglobin formation by thiols

Smith, Robert C.; Reed, Villel D.; Webb, Thomas R.

doi:10.1016/0162-0134(93)80038-b

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…Methemoglobin forms when the ferrous iron in oxyhemoglobin is oxidized to the ferric form, thus preventing oxygen binding and oxygen transport in vivo. In this study, methemoglobin formation was indicated by a change in the color of the blood from red to brown, and by development of a characteristic absorption spectrum with decreased absorption at 576 and 540 nm, and increased absorption at 625–630 nm . Although the spectrophotometric absorption spectrum of methemoglobin is usually described as having a characteristic peak at 630 nm, peaks in this study were typically closer to 625 nm.…”

Section: Discussionmentioning

confidence: 78%

“…Aliquots of the resulting supernatants were diluted with water, transferred to quartz cuvettes, and spectral absorbances were measured at 350–850 nm using a Shimadzu UV‐Visible Recording Spectrophotometer UV‐160U (Shimadzu, Columbia, MD, USA), a microcomputer‐controlled double‐beam instrument, in accordance with the manufacturer's instructions. Aliquots were assessed for the expected characteristic pattern of methemoglobin formation: increased absorption at approximately 630 and 500 nm, and concurrent reduction in oxyhemoglobin‐associated absorption at approximately 576 and 540 nm …”

Section: Methodsmentioning

confidence: 99%

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Skunk musk causes methemoglobin and Heinz body formation in vitro

Fierro

Agnew

Duncan³

et al. 2013

Veterinary Clinical Pathol

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Section: Discussionmentioning

confidence: 78%

Section: Methodsmentioning

confidence: 99%

Skunk musk causes methemoglobin and Heinz body formation in vitro

Fierro

Agnew

Duncan³

et al. 2013

Veterinary Clinical Pathol

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“…In addition, a direct action of metals on the earthworm haeme group cannot be excluded. In fact, copper is a known direct-acting methemoglobin-producing agent in humans directly converting Fe(II) to Fe(III) in a two-stage reaction (Smith and Reed 1993;French et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Effect of heavy metal exposure on blood haemoglobin concentration and methemoglobin percentage in Lumbricus terrestris

et al. 2011

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The earthworm haemoglobin (Hb) is a large extracellular hemoprotein flowing in a closed circulatory system. In spite of the fundamental role of this respiratory pigment in earthworm physiology, little is known about its sensitivity to environmental pollutants. The aim of the present work was to investigate the possible effect of heavy metal (cadmium, copper, mercury) exposure on Hb concentration and oxidation state (methemoglobin formation) in the earthworm Lumbricus terrestris. In addition, the tissue concentration of metallothioneins, a well-known biomarker of heavy metal exposure, was determined as an indicator of metal uptake. The animals were exposed to increasing concentrations of Cd, Cu and Hg utilizing the standard acute toxicity test, "Filter paper test" for 48 h. Exposure to heavy metals (10(-5)-10(-3) M for Cd, 10(-4)-10(-3) M for Hg, and 10(-4)-10(-2) M for Cu) was found to increase haemoglobin concentration in L. terrestris, although the magnitude of such an increase was dependent on the metal. In addition, metal exposure led to the formation of methemoglobin. Compared to other known biological responses to heavy metals, such as metallothionein induction, methemoglobin increase showed a higher sensitivity and a higher percentage variation in exposed organisms, showing to be a possible suitable biomarker of exposure/effect to be included in a multi biomarker strategy in earthworm in soil monitoring assessment.

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“…In addition, a direct action of metals on the earthworm haeme group cannot be excluded. In fact, copper is a known direct-acting methemoglobinproducing agent in humans directly converting Fe(II) to Fe(III) in a two-stage reaction (Smith & Reed 1993, French et al 1995. Compared to other biological responses to heavy metals, such as the known metallothionein induction, MetHb increase showed a higher sensitivity.…”

Section: Haemoglobin Oxidationmentioning

confidence: 99%