Revealing the sedative-hypnotic effect of the extracts of herb pair Semen Ziziphi spinosae and Radix Polygalae and related mechanisms through experiments and metabolomics approach

Abstract

Background: Semen Ziziphi spinosae and Radix Polygalae, two herbs commonly used together in Traditional Chinese Medicine for the treatment of insomnia and anxiety. The study aims to study the sedative-hypnotic effect of the active components of the herbal pair, the possible mechanisms of such effect, and related metabolic pathways in vivo. Methods: The sedative and hypnotic effect of the active components (EI30) of the herbal pair was studied by recording influence on the proportion of sleeping within 30 min, sleep latency and sleep length of pentobarbital sodium-induced sleeping on mice. Possible mechanisms of the sedative-hypnotic effect of the active components were investigated by measuring the content of neurotransmitters in the total protein of mice brain tissue. The main chemical compounds of the herbal pair were identified by Liquid Chromatography-Mass Spectrometry (LC-MS). Serum samples of mice were studied, and related differential metabolites between the normal group and model group, and between model group and treatment group were identified by Gas Chromatography Time-Of-Flight Mass Spectrometry (GC-TOF-MS), Principal Components Analysis (PCA), and Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA).

3,6'‐disinapoyl sucrose attenuates Aβ1‐42‐ induced neurotoxicity in Caenorhabditis elegans by enhancing antioxidation and regulating autophagy

“…11 Modern pharmacological studies showed that the extracts of Polygalae Radix could prevent glutamate, Aβ and corticosteroneinduced cell injury, improve dysfunction of learning and memory in multiple AD animal models, mediate depression and exert sedativehypnotic effect. [12][13][14][15][16] 3,6'-disinapoyl sucrose (DISS) is a kind of oligosaccharide ester derived from Radix Polygala, which has been used as one of the quality control indices of Polygalae Radix in the Chinese Pharmacopoeia (2015 edition). 11 DISS has antioxidative effects, neuroprotection and anti-depression in cells and animal model.…”

Effect of Chinese Herbs on Serum Biochemical Parameters, Immunity Indices, Antioxidant Capacity and Metabolomics in Early Weaned Yak Calves

“…Phenylalanine, via phenylalanine hydroxylase, produces tyrosine, which produces the neurotransmitter dopamine [ 69 ]. The ethanol eluent of Semen ziziphi and Radix polygalae down-regulated phenylalanine, mainly due to the sedative-hypnotic role of the flavonoid and saponin contents [ 70 ]. Glycosides of C. pilosula act as a sedative agent [ 71 ].…”

Efficacy of integrated traditional Chinese medicine and Western medicine in the treatment of poststroke insomnia

“…They expounded the action ways of Chinese medicine in regulating central neurotransmitter affecting sleep-related cytokines, and improving the structure of the central nervous system. [ 18 , 19 ]…”