Revealing the Architecture of the Cell Wall in Living Plant Cells by Bioimaging and Enzymatic Degradation

Yilmaz, Neval; Kodama, Yutaka; Numata, Keiji

doi:10.1021/acs.biomac.9b00979

Cited by 24 publications

(30 citation statements)

References 48 publications

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“…Consistent with these data, ruthenium red staining demonstrated that BY-2 cells have abundant pectin on their surface. Our findings agree with previous work that indicated that BY-2 cells produce a layer of pectin after subculture ( 59 ). Additional work is required to uncover the mechanism by which pectin mediates the adhesion of plant cells to scaffolds.…”

Section: Discussionsupporting

confidence: 93%

Plant cell adhesion and growth on artificial fibrous scaffolds as an in vitro model for plant development

et al. 2021

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Section: Discussionsupporting

confidence: 93%

Plant cell adhesion and growth on artificial fibrous scaffolds as an in vitro model for plant development

et al. 2021

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“…Consistent with this data, ruthenium red staining demonstrated that BY-2 cells have abundant pectin on their surface. Our findings are in agreement with previous work that indicated that BY-2 cells produce a layer of pectin around four days after subculture 43 . Since the adhesion of BY-2 cells to scaffolds is reminiscent of the way cells adhere in plant tissues, our scaffolds should be suitable for plant tissue engineering.…”

Section: Cell-cell Adhesion and Electrical Signaling Between Cells Insupporting

confidence: 94%

Artificial scaffolds that mimic the plant extracellular environment for the culture and attachment of plant cells

Calcutt

Vincent

Dean

et al. 2020

Preprint

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Plant growth and development involves an intricate program of cell division and cell expansionto generate different cell types, tissue patterns and organ shapes. Plant cells are stuck together by their cell walls and the spatial context of cells within tissues plays a critical role in cell fate specification and morphogenesis. An in vitro model system to study plant development and its regulation by various extrinsic and intrinsic factors requires the ability to mimic the physical interactions between cells and their environment. Here, we present a set of artificial scaffolds to which cultured tobacco BY-2 cells adhere without causing morphological abnormalities. These scaffolds mimic native plant cell walls in terms of their fibrous nature, charge, hydrophobicity and piezoelectricity. We found that the extent of plant cell adhesion was essentially insensitive to the stiffness, fiber dimension, and fiber orientation of the scaffolds, but was affected by the piezoelectric properties of scaffolds where adhesion increased on piezoelectric materials. We also found that the plant cell wall polysaccharide, pectin, is largely responsible for adhesion to scaffolds, analogous to pectin-mediated adhesion of plant cells in tissues. Together, this work establishes biomimetic scaffolds that realistically emulate the plant tissue environment and provide the capability to develop microfluidic devices to study how cell-cell and cell-matrix interactions affect plant developmental pathways.

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Section: Recent Progress In the Fundamental Understanding Of Nanocellmentioning

confidence: 99%

“…[ 26 ] The shift in the microfibril orientation between the layers appeared to be random rather than gradual. [ 26–28 ]…”

Section: Recent Progress In the Fundamental Understanding Of Nanocellmentioning

confidence: 99%

Nanocellulose: Recent Fundamental Advances and Emerging Biological and Biomimicking Applications

et al. 2020

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In the effort toward sustainable advanced functional materials, nanocelluloses have attracted extensive recent attention. Nanocelluloses range from rod‐like highly crystalline cellulose nanocrystals to longer and more entangled cellulose nanofibers, earlier denoted also as microfibrillated celluloses and bacterial cellulose. In recent years, they have spurred research toward a wide range of applications, ranging from nanocomposites, viscosity modifiers, films, barrier layers, fibers, structural color, gels, aerogels and foams, and energy applications, until filtering membranes, to name a few. Still, nanocelluloses continue to show surprisingly high challenges to master their interactions and tailorability to allow well‐controlled assemblies for functional materials. Rather than trying to review the already extensive nanocellulose literature at large, here selected aspects of the recent progress are the focus. Water interactions, which are central for processing for the functional properties, are discussed first. Then advanced hybrid gels toward (multi)stimuli responses, shape‐memory materials, self‐healing, adhesion and gluing, biological scaffolding, and forensic applications are discussed. Finally, composite fibers are discussed, as well as nanocellulose as a strategy for improvement of photosynthesis‐based chemicals production. In summary, selected perspectives toward new directions for sustainable high‐tech functional materials science based on nanocelluloses are described.

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Revealing the Architecture of the Cell Wall in Living Plant Cells by Bioimaging and Enzymatic Degradation

Cited by 24 publications

References 48 publications

Plant cell adhesion and growth on artificial fibrous scaffolds as an in vitro model for plant development

Plant cell adhesion and growth on artificial fibrous scaffolds as an in vitro model for plant development

Artificial scaffolds that mimic the plant extracellular environment for the culture and attachment of plant cells

Nanocellulose: Recent Fundamental Advances and Emerging Biological and Biomimicking Applications

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