Revealing microbial mechanism associated with volatile fatty acids production in anaerobic acidogenesis of waste activated sludge enhanced by freezing/thawing pretreatment

She, Yuecheng; Hong, Ji-Hyung; Zhang, Qian; Chen, Bor-Yann; Wei, Wenxuan; Xin, Xiaodong

doi:10.1016/j.biortech.2020.122869

Cited by 58 publications

(15 citation statements)

References 46 publications

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“…Fermentation commonly occurs in anaerobic bioreactors, and the key step in the biotransformation of organic micropollutants is acidogenesis [ 31 ]. VFAs are the major intermediate metabolites during anaerobic digestion [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…Detected Propionigenium -like microbes (Fusobacteria) are potentially secondary fermenters that preferably use decarboxylic acids as substrates in their fermentative metabolism to produce propionate [ 50 ]. Firmicutes-, Bacteroidetes-, Verrucomicrobia-, Caldithrix-, and OD1-affiliated microbes are the most common found phyla during acidogenesis in bioreactors [ 30 , 32 , 51 , 52 ]. According to the blast results obtained using the NCBI database, all the above representative OTUs were affiliated with reported anaerobic microbes ( Fig 5 ).…”

Section: Resultsmentioning

confidence: 99%

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Elucidate microbial characteristics in a full-scale treatment plant for offshore oil produced wastewater

et al. 2021

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Oil-produced wastewater treatment plants, especially those involving biological treatment processes, harbor rich and diverse microbes. However, knowledge of microbial ecology and microbial interactions determining the efficiency of plants for oil-produced wastewater is limited. Here, we performed 16S rDNA amplicon sequencing to elucidate the microbial composition and potential microbial functions in a full-scale well-worked offshore oil-produced wastewater treatment plant. Results showed that microbes that inhabited the plant were diverse and originated from oil and marine associated environments. The upstream physical and chemical treatments resulted in low microbial diversity. Organic pollutants were digested in the anaerobic baffled reactor (ABR) dominantly through fermentation combined with sulfur compounds respiration. Three aerobic parallel reactors (APRs) harbored different microbial groups that performed similar potential functions, such as hydrocarbon degradation, acidogenesis, photosynthetic assimilation, and nitrogen removal. Microbial characteristics were important to the performance of oil-produced wastewater treatment plants with biological processes.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Elucidate microbial characteristics in a full-scale treatment plant for offshore oil produced wastewater

et al. 2021

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“…Although VFAs were also the main components in CW, they were dominant only in CW67 when acetoclastic methanogenesis was activated. The accumulation of VFAs from CW34 to CW67 would be due to the action of such VFAs producing bacteria as Acetoanaerobium and Macellibacteroides besides fungi. However, there were few reports of H 2 production by Acetoanaerobium and Macellibacteroides , while Acetoanaerobium even produce acetic acid by consuming H 2 , which could lead to further reduction of H 2 in the system and improve the activity of acetoclastic methanogenesis.…”

Section: Resultsmentioning

confidence: 99%

High Potential of Methane Production from Coal by Fungi and Hydrogenotrophic Methanogens from Produced Water

et al. 2020

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Studying in situ microorganisms is essential to understand the formation of biogenic coalbed methane (CBM). Only a few studies have investigated the fungi community inhabiting in coal seams although fungi are found to degrade refractory compounds and solubilize coal and even work with methanogens to produce methane. In this study, produced water was collected from Qinshui Basin and used as the source of microflora to degrade coal. The function of fungi was analyzed by inhibition of bacteria with antibiotics. The results have shown that the inhibition of bacteria in the microcosms significantly increased the methane yield. Fungi were more favorable to cooperate with hydrogenotrophic methanogens when bacteria were inhibited as Methanobacterium were the predominant archaea accounting for 45.98–86.98% of the sequence reads. The relatively unchanged fungal community and high volatile fatty acids (VFAs) yields (48.70–85.72%) in the presence of antibiotics might contribute to H2 production to facilitate hydrogenotrophic methanogenesis. On the contrary, the dominant methanogens in microcosms without antibiotics gradually changed from Methanobacterium (89.85%) to Methanoceta (46.19%) and finally to Methanobacterium (87.72%). The bacterial community and organic intermediates have also changed greatly over time, which can be the reason for the succession of the methanogens. These results suggest that fungi in produced water can play an important role in coal biodegradation to produce VFAs and H2 that support hydrogenotrophic methanogenesis, which could be negatively impacted by the existence of bacteria.

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“…Their results stated that the highest acetic acid concentration was obtained as 918 mg COD/L at day 30 from 33400 mg COD/L content of the substrate. She et al (2020) used the pretreatment (freezing/thawing) for VFA production from waste activated sludge. The pretreatment increased the acetic acid production from 933 ± 46 to 1281 ± 57 mg COD/L as maximum concentration (the SCOD concentration of substrate was 5852 mg/L) (She et al, 2020).…”

Section: Acetic Acid Dominant Vfa Productionmentioning

confidence: 99%

“…She et al (2020) used the pretreatment (freezing/thawing) for VFA production from waste activated sludge. The pretreatment increased the acetic acid production from 933 ± 46 to 1281 ± 57 mg COD/L as maximum concentration (the SCOD concentration of substrate was 5852 mg/L) (She et al, 2020). From the view of substrate concentration in terms of COD content, the bioaugmentation of C. aceticum enhanced acetic acid production via fermentation more than 10 times than other studies.…”

Section: Acetic Acid Dominant Vfa Productionmentioning

confidence: 99%

Bioaugmented Mixed Culture by Clostridium aceticum to Manipulate Volatile Fatty Acids Composition From the Fermentation of Cheese Production Wastewater

Atasoy

Çetecioğlu

2021

Front. Microbiol.

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Production of targeted volatile fatty acid (VFA) composition by fermentation is a promising approach for upstream and post-stream VFA applications. In the current study, the bioaugmented mixed microbial culture by Clostridium aceticum was used to produce an acetic acid dominant VFA mixture. For this purpose, anaerobic sequencing batch reactors (bioaugmented and control) were operated under pH 10 and fed by cheese processing wastewater. The efficiency and stability of the bioaugmentation strategy were monitored using the production and composition of VFA, the quantity of C. aceticum (by qPCR), and bacterial community profile (16S rRNA Illumina Sequencing). The bioaugmented mixed culture significantly increased acetic acid concentration in the VFA mixture (from 1170 ± 18 to 122 ± 9 mgCOD/L) compared to the control reactor. Furthermore, the total VFA production (from 1254 ± 11 to 5493 ± 36 mgCOD/L) was also enhanced. Nevertheless, the bioaugmentation could not shift the propionic acid dominancy in the VFA mixture. The most significant effect of bioaugmentation on the bacterial community profile was seen in the relative abundance of the Thermoanaerobacterales Family III. Incertae sedis, its relative abundance increased simultaneously with the gene copy number of C. aceticum during bioaugmentation. These results suggest that there might be a syntropy between species of Thermoanaerobacterales Family III. Incertae sedis and C. aceticum. The cycle analysis showed that 6 h (instead of 24 h) was adequate retention time to achieve the same acetic acid and total VFA production efficiency. Biobased acetic acid production is widely applicable and economically competitive with petroleum-based production, and this study has the potential to enable a new approach as produced acetic acid dominant VFA can replace external carbon sources for different processes (such as denitrification) in WWTPs. In this way, the higher treatment efficiency for WWTPs can be obtained by recovered substrate from the waste streams that promote a circular economy approach.

show abstract

Revealing microbial mechanism associated with volatile fatty acids production in anaerobic acidogenesis of waste activated sludge enhanced by freezing/thawing pretreatment

Cited by 58 publications

References 46 publications

Elucidate microbial characteristics in a full-scale treatment plant for offshore oil produced wastewater

Elucidate microbial characteristics in a full-scale treatment plant for offshore oil produced wastewater

High Potential of Methane Production from Coal by Fungi and Hydrogenotrophic Methanogens from Produced Water

Bioaugmented Mixed Culture by Clostridium aceticum to Manipulate Volatile Fatty Acids Composition From the Fermentation of Cheese Production Wastewater

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