Reveal the response of enzyme activities to heavy metals through in situ zymography

Duan, Chengjiao; Fang, Linchuan; Yang, Congli; Chen, Weibin; Cui, Yongxing; Li, Shiqing

doi:10.1016/j.ecoenv.2018.03.015

Cited by 191 publications

(52 citation statements)

References 46 publications

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“…In both CK1 and CK2, CAT enzyme inhibition occurred to a great extent and comparing to the controls, plants grown in amended treatments especially with higher amendment gradients could activate CAT mechanism to counteract metal induced excess H 2 O 2 . Though antioxidant enzyme activities in plant cells supposed to be enhanced under metal stress as a defensive mechanism to combat oxidative stress, many studies reported the declined CAT activity in plant tissues under metal stress due to metal concentrations beyond physiological tolerance level [81][82][83]. In a recent study, Zhang and Ji [84] observed decreased CAT activity in Orychophragmus violaceus, Festuca arundinacea and Medicago sativa under higher stress of metals, which is in support to our findings.…”

Section: Effect Of Amendments On Antioxidant Enzyme Activities and Sosupporting

confidence: 91%

Phytostabilization of Pb-Zn Mine Tailings with Amorpha fruticosa Aided by Organic Amendments and Triple Superphosphate

et al. 2020

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A greenhouse pot trial was conducted to investigate the effect of organic amendments combined with triple superphosphate on the bioavailability of heavy metals (HMs), Amorpha fruticosa growth and metal uptake from Pb-Zn mine tailings. Cattle manure compost (CMC), spent mushroom compost (SMC) and agricultural field soil (AFS) were applied to tailings at 5%, 10%, 20% and 30% w/w ratio, whereas sewage sludge (SS) and wood biochar (WB) were mixed at 2.5%, 5%, 10% and 20% w/w ratio. Triple superphosphate (TSP) was added to all the treatments at 4:1 (molar ratio). Amendments efficiently decreased DTPA-extracted Pb, Zn, Cd and Cu in treatments. Chlorophyll contents and shoot and root dry biomass significantly (p < 0.05) increased in the treatments of CMC (except T4 for chlorophyll b) and SMC, whereas treatments of SS (except T1 for chlorophyll a and b), WB and AFS (except T4 for chlorophyll a and b) did not show positive effects as compared to CK1. Bioconcentration factor (BCF) and translocation factor (TF) values in plant tissues were below 1 for most treatments. In amended treatments, soluble protein content increased, phenylalanine ammonialyase (PAL) and polyphenol oxidase (PPO) decreased, and catalase (CAT) activity showed varied results as compared to CK1 and CK2. Results suggested that A. fruticosa can be a potential metal phytostabilizer and use of CMC or SMC in combination with TSP are more effective than other combinations for the in situ stabilization of Pb-Zn mine tailings.

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Section: Effect Of Amendments On Antioxidant Enzyme Activities and Sosupporting

confidence: 91%

Phytostabilization of Pb-Zn Mine Tailings with Amorpha fruticosa Aided by Organic Amendments and Triple Superphosphate

et al. 2020

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“…e superfluous accumulation of ROS (reactive oxygen species) caused oxidative stress in plants, especially for sensitive cellular membranes. MDA is the ultimate decomposition product of membrane lipid peroxidation, which is the expression of the degree of peroxidation of plant cell membrane, and its content can reflect the degree of damage of the plant [42]. e higher the content of MDA indicated that the higher the degree of peroxidation of the plant cell membrane, the more serious damage to the cell membrane.…”

Section: + -N Nomentioning

confidence: 99%

Intercropping of Gramineous Pasture Ryegrass (Lolium perenne L.) and Leguminous Forage Alfalfa (Medicago sativa L.) Increases the Resistance of Plants to Heavy Metals

Cui

Fang

Wang

et al. 2018

Journal of Chemistry

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Intercropping can increase the biomass of plants and reduce the accumulation of heavy metals in plants. However, the mechanisms of intercropping increasing plant biomass and resistance to heavy metals are still unclear. Therefore, the pot experiment had been conducted to investigate the effect of intercropping treatment on the growth of gramineous pasture ryegrass (Lolium perenne L.) and leguminous forage alfalfa (Medicago sativa L.) in metal-contaminated soil. Our results showed that intercropping alleviated inhibition of heavy metals to plant growth and increased nitrogen and chlorophyll contents in the shoots and roots. Moreover, the Pb concentrations in the shoots and roots of ryegrass and alfalfa in the intercropping were significantly lower than those in the monoculture. And, the contents of saccharase and alkaline phosphatase were significantly increased in the intercropping treatment. Additionally, the intercropping treatment could reduce the oxidative damage and increase enzymatic antioxidant activities to improve the resistance of plants in contaminated soil. The intercropping treatment can increase the resistance of plants to heavy metals through reduction of plant oxidative damage and increase of antioxidant activity. It could provide us with a strategy that intercropping of ryegrass and alfalfa can increase biomass and reduce the absorption of Pb on forage plants.

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“…Application of biodegradable chelator for washing the polluted soil mitigated the oxidative stress in B. bara and L. sativa (Fig. 3 and Table 1), possibly by decreasing the total metal concentrations and plantÀavailable Cd, Pb, and Zn concentrations (Bashir et al, 2017;Duan et al, 2018).…”

Section: Antioxidant Defense Systemmentioning

confidence: 99%

Feasibility of Chinese cabbage (Brassica bara) and lettuce (Lactuca sativa) cultivation in heavily metals−contaminated soil after washing with biodegradable chelators

Wang

Zhang

Zhong

et al. 2018

Journal of Cleaner Production

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Reveal the response of enzyme activities to heavy metals through in situ zymography

Cited by 191 publications

References 46 publications

Phytostabilization of Pb-Zn Mine Tailings with Amorpha fruticosa Aided by Organic Amendments and Triple Superphosphate

Phytostabilization of Pb-Zn Mine Tailings with Amorpha fruticosa Aided by Organic Amendments and Triple Superphosphate

Intercropping of Gramineous Pasture Ryegrass (Lolium perenne L.) and Leguminous Forage Alfalfa (Medicago sativa L.) Increases the Resistance of Plants to Heavy Metals

Feasibility of Chinese cabbage (Brassica bara) and lettuce (Lactuca sativa) cultivation in heavily metals−contaminated soil after washing with biodegradable chelators

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