Phytostabilization of Pb-Zn Mine Tailings with Amorpha fruticosa Aided by Organic Amendments and Triple Superphosphate

Abstract

A greenhouse pot trial was conducted to investigate the effect of organic amendments combined with triple superphosphate on the bioavailability of heavy metals (HMs), Amorpha fruticosa growth and metal uptake from Pb-Zn mine tailings. Cattle manure compost (CMC), spent mushroom compost (SMC) and agricultural field soil (AFS) were applied to tailings at 5%, 10%, 20% and 30% w/w ratio, whereas sewage sludge (SS) and wood biochar (WB) were mixed at 2.5%, 5%, 10% and 20% w/w ratio. Triple superphosphate (TSP) was added to all the treatments at 4:1 (molar ratio). Amendments efficiently decreased DTPA-extracted Pb, Zn, Cd and Cu in treatments. Chlorophyll contents and shoot and root dry biomass significantly (p < 0.05) increased in the treatments of CMC (except T4 for chlorophyll b) and SMC, whereas treatments of SS (except T1 for chlorophyll a and b), WB and AFS (except T4 for chlorophyll a and b) did not show positive effects as compared to CK1. Bioconcentration factor (BCF) and translocation factor (TF) values in plant tissues were below 1 for most treatments. In amended treatments, soluble protein content increased, phenylalanine ammonialyase (PAL) and polyphenol oxidase (PPO) decreased, and catalase (CAT) activity showed varied results as compared to CK1 and CK2. Results suggested that A. fruticosa can be a potential metal phytostabilizer and use of CMC or SMC in combination with TSP are more effective than other combinations for the in situ stabilization of Pb-Zn mine tailings.

Contrasting effects of maize residue, coal gas residue and their biochars on nutrient mineralization, enzyme activities and CO2 emissions in sandy loess soil

“…Similar, fluctuations were documented by incubation and pot experiment studies ( El-Mahrouky et al, 2015 , Zhu et al, 2017 , Zhu et al, 2017 , Sadaf et al, 2017 , Sial et al, 2019a , Khan et al, 2019 ) they investigated that organic waste release of acidic decomposable compounds and decreased soil EC and pH. Whereas, biochar application increase soil EC and pH due its liming effects on soil environment ( Sial et al, 2019b , Sikdar et al, 2020 ). Biochar applications increased soil EC and pH ( Khan et al, 2019 ), liming effects ( Randolph et al, 2017 ) and alkalinity is a major aspect contributed liming potential ( Zhu et al, 2017 , Zhu et al, 2017 ), and caused increasing in soil EC and pH ( Sial et al, 2019d ).…”

Carbonation, Cementation, and Stabilization of Ultramafic Mine Tailings

“…Numerous studies have investigated the addition of organics including compost, biosolids, and organic-rich sludge to tailings for the purpose of mine reclamation, technosol formation, phytostabilization, and rehabilitation. − The addition of organics to mine tailings has also been shown to induce biogeochemical processes such as sulfate reduction, which can immobilize elements of concern (e.g., As) through the precipitation of sulfide minerals . In this study, coupling organic and inorganic carbon cycling dramatically accelerated brucite carbonation, which is often limited by CO 2 supply.…”

Exogenous Melatonin Modulates the Physiological and Biochemical Mechanisms of Drought Tolerance in Tartary Buckwheat (Fagopyrum tataricum (L.) Gaertn)

“…PAL acts as the major enzyme for the conversion of L-phenyalanine into trans-cinnamic acid in the phenylpropanoid pathway, which subsequently produces phenolic compounds like phenols and flavonoids [ 13 , 53 , 54 ]. Our results revealed that PAL activity improved under stress conditions relative to the control plants ( Figure 6 A) [ 12 ].…”