Reuse of immobilized lipases in the transesterification of waste fish oil for the production of biodiesel

Marín-Suárez, Marta; Méndez-Mateos, David; Guadix, Antonio; Guadix, Emilia M.

doi:10.1016/j.renene.2019.03.035

Cited by 82 publications

(42 citation statements)

References 36 publications

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“…Recently, Jayaraman et al 423 demonstrated the lipase enzyme-mediated transesterication of waste cooking oil (WCO), and reported 88% of biodiesel yield. Marín-Suárez et al 424 demonstrated the lipase-catalyzed transesterication of low quality sh oil through the process optimization. Moreover, the reusability of the enzyme was also studied.…”

Section: Enzyme Catalystmentioning

confidence: 99%

Widely used catalysts in biodiesel production: a review

et al. 2020

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Section: Enzyme Catalystmentioning

confidence: 99%

Widely used catalysts in biodiesel production: a review

et al. 2020

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“…Marín-Suárez et al studied the transesterification of waste fish oil for biodiesel production using three commercial immobilized enzymes: Lipozyme RM IM, Lipozyme TL IM, and Novozym 435 [44]. They reported the highest biodiesel yield of 82.91% with 50% Novozyme 435 using excess ethanol per mol of oil.…”

Section: Enzyme Concentrationmentioning

confidence: 99%

Statistical Optimization of Biodiesel Production from Salmon Oil via Enzymatic Transesterification: Investigation of the Effects of Various Operational Parameters

et al. 2021

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The enzymatic transesterification of Atlantic salmon (Salmo salar) oil was carried out using Novozym 435 (immobilized lipase from Candida antartica) to produce biodiesel. A response surface modelling design was performed to investigate the relationship between biodiesel yield and several critical factors, including enzyme concentration (5, 10, or 15%), temperature (40, 45, or 50 °C), oil/alcohol molar ratio (1:3, 1:4, or 1:5) and time (8, 16, or 24 h). The results indicated that the effects of all the factors were statistically significant at p-values of 0.000 for biodiesel production. The optimum parameters for biodiesel production were determined as 10% enzyme concentration, 45 °C, 16 h, and 1:4 oil/alcohol molar ratio, leading to a biodiesel yield of 87.23%. The step-wise addition of methanol during the enzymatic transesterification further increased the biodiesel yield to 94.5%. This is the first study that focused on Atlantic salmon oil-derived biodiesel production, which creates a paradigm for valorization of Atlantic salmon by-products that would also reduce the consumption and demand of plant oils derived from crops and vegetables.

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“…It can be seen that the best conversion to ethyl esters was found in the 1:1 molar ratio and that it did not significantly increase the conversion value in larger molar ratios. Lipozyme RM-IM was studied, for the production of fatty acid ethyl ester using fish oil and ethanol as a substrate, the authors concluded that the highest yields were found in the lowest 1:0.25 molar ratio (fish oil/ethanol), showing the low resistance of the enzyme to the presence of alcohol (Marín-Suárez et al, 2019). On the other hand, unlike the other parameters, time did not influence the conversion values in the interval between 2 and 6 h, as shown in Table 5.…”

Section: Optimization Of the Production Of Fatty Acid Ethyl Estermentioning

confidence: 99%

Lipase From Rhizomucor miehei Immobilized on Magnetic Nanoparticles: Performance in Fatty Acid Ethyl Ester (FAEE) Optimized Production by the Taguchi Method

Moreira

Oliveira

Júnior

et al. 2020

Front. Bioeng. Biotechnol.

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In this communication, it was evaluated the production of fatty acid ethyl ester (FAAE) from the free fatty acids of babassu oil catalyzed by lipase from Rhizomucor miehei (RML) immobilized on magnetic nanoparticles (MNP) coated with 3-aminopropyltriethoxysilane (APTES), Fe 3 O 4 @APTES-RML or RML-MNP for short. MNPs were prepared by co-precipitation coated with 3-aminopropyltriethoxysilane and used as a support to immobilize RML (immobilization yield: 94.7 ± 1.0%; biocatalyst activity: 341.3 ± 1.2 U p−NPB /g), which were also activated with glutaraldehyde and then used to immobilize RML (immobilization yield: 91.9 ± 0.2%; biocatalyst activity: 199.6 ± 3.5 U p−NPB /g). RML-MNP was characterized by X-Ray Powder Diffraction (XRPD), Fourier Transform-Infrared (FTIR) spectroscopy and Scanning Electron Microscope (SEM), proving the incorporation and immobilization of RML on the APTES matrix. In addition, the immobilized biocatalyst presented at 60 • C a half-life 16-19 times greater than that of the soluble lipase in the pH range 5-10. RML and RML-MNP showed higher activity at pH 7; the immobilized enzyme was more active than the free enzyme in the pH range (5-10) analyzed. For the production of fatty acid ethyl ester, under optimal conditions [40 • C, 6 h, 1:1 (FFAs/alcohol)] determined by the Taguchi method, it was possible to obtain conversion of 81.7 ± 0.7% using 5% of RML-MNP.

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Reuse of immobilized lipases in the transesterification of waste fish oil for the production of biodiesel

Cited by 82 publications

References 36 publications

Widely used catalysts in biodiesel production: a review

Widely used catalysts in biodiesel production: a review

Statistical Optimization of Biodiesel Production from Salmon Oil via Enzymatic Transesterification: Investigation of the Effects of Various Operational Parameters

Lipase From Rhizomucor miehei Immobilized on Magnetic Nanoparticles: Performance in Fatty Acid Ethyl Ester (FAEE) Optimized Production by the Taguchi Method

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