Reusable gold nanoparticle enhanced QCM immunosensor for detecting C-reactive protein

Ding, Pengfei; Liu, Shihui; Mao, Xueyin; Hu, Ruifen; Li, Guang

doi:10.1016/j.snb.2013.07.099

Cited by 32 publications

(15 citation statements)

References 29 publications

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“…This QCM was tested with CEA, human IgG and PSA, but these molecules did not provide any interfering signal. Gold nanoparticles were also used in a QCM working at higher concentrations [ 105 ]. The QCM surface was coated with capture anti-CRP, whereas the detection anti-CRP were anchored to gold nanoparticles.…”

Section: Sensors For C-reactive Proteinmentioning

confidence: 99%

Sensors and Biosensors for C-Reactive Protein, Temperature and pH, and Their Applications for Monitoring Wound Healing: A Review

Salvo

Dini

Kirchhain

et al. 2017

Sensors

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Wound assessment is usually performed in hospitals or specialized labs. However, since patients spend most of their time at home, a remote real time wound monitoring would help providing a better care and improving the healing rate. This review describes the advances in sensors and biosensors for monitoring the concentration of C-reactive protein (CRP), temperature and pH in wounds. These three parameters can be used as qualitative biomarkers to assess the wound status and the effectiveness of therapy. CRP biosensors can be classified in: (a) field effect transistors, (b) optical immunosensors based on surface plasmon resonance, total internal reflection, fluorescence and chemiluminescence, (c) electrochemical sensors based on potentiometry, amperometry, and electrochemical impedance, and (d) piezoresistive sensors, such as quartz crystal microbalances and microcantilevers. The last section reports the most recent developments for wearable non-invasive temperature and pH sensors suitable for wound monitoring.

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Section: Sensors For C-reactive Proteinmentioning

confidence: 99%

Sensors and Biosensors for C-Reactive Protein, Temperature and pH, and Their Applications for Monitoring Wound Healing: A Review

Salvo

Dini

Kirchhain

et al. 2017

Sensors

View full text Add to dashboard Cite

show abstract

“…In the clinical laboratories, enzyme-linked immunosorbent assay (ELISA), turbidimetry, and nephelometry are the major methods used for quantifying the concentration of CRP in human samples ( Dominici et al, 2004 ; Clarke et al, 2005 ; Parra et al, 2005 ; Vilian et al, 2019 ). Although ELISA is a sensitive technique, it is time-consuming and expensive and requires bulky equipment and highly skilled personnel for performing the analysis and result interpretation ( Ding et al, 2013 ). These requirements make ELISA technique not suitable for diagnosis, especially in low-resource settings.…”

Section: Introductionmentioning

confidence: 99%

Oriented Antibody Covalent Immobilization for Label-Free Impedimetric Detection of C-Reactive Protein via Direct and Sandwich Immunoassays

Adesina

Mashazi

2021

Front. Chem.

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The detection and monitoring of biological markers as disease indicators in a simple manner is a subject of international interest. In this work, we report two simple and sensitive label-free impedimetric immunoassays for the detection of C-reactive protein (CRP). The gold electrode modified with boronic acid–terminated self-assembled monolayers afforded oriented immobilization of capture glycosylated antibody (antihuman CRP monoclonal antibody, mAb). This antibody-modified surface was able to capture human CRP protein, and the impedance signal showed linear dependence with CRP concentration. We confirmed the immobilization of anti-CRP mAb using surface sensitive X-ray photoelectron spectroscopy (XPS) and electrochemical impedance. The oriented covalent immobilization of mAb was achieved using glycosylated Fc (fragment, crystallizable) region specific to boronic acid. The direct immunoassay exhibited a linear curve for concentration range up to 100 ng ml−1. The limit of detection (LoD) of 2.9 ng ml−1, limit of quantification (LoQ) of 9.66 ng ml−1, and sensitivity of 0.585 kΩ ng−1 ml cm−2 were obtained. The sandwich immunoassay was carried out by capturing polyclonal anti-CRP antibody (pAb) onto the CRP antigen immunoreaction. The impedance signal after pAb capture also showed linear dependence with CRP antigen concentration and acted as a CRP antigen detection signal amplifier. The detection of the CRP antigen using sandwich pAb immunoassay improved LoD to 1.2 ng ml−1, LoQ to 3.97 ng ml−1, and enhanced the sensitivity to 0.885 kΩ ng−1 ml cm−2. The real sample analysis, using newborn calf serum, showed excellent selectivity and % recovery for the human CRP ranging from 91.2 to 96.5%. The method was reproducible to 4.5% for direct immunoassay and 2.3% for sandwich immunoassay.

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“…In QCM experiments, for example, the mass-amplified sandwich assays were performed for high sensitive detection of biomarkers. [14][15][16] However, the sandwich assay requires several extra procedures and deteriorates the important advantage of the label-free biosensor, that is, the short-time and real-time assay.…”

Section: Introductionmentioning

confidence: 99%

Ultrahigh-Frequency, Wireless MEMS QCM Biosensor for Direct, Label-Free Detection of Biomarkers in a Large Amount of Contaminants

et al. 2019

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Label-free biosensors, including conventional quartz-crystal-microbalance (QCM) biosensor, are seriously affected by nonspecific adsorption of contaminants involved in analyte solution, and it is exceptionally difficult to extract the sensor responses caused only by the targets. In this study, we reveal that this difficulty can be overcome with an ultrahigh-frequency wireless QCM biosensor. The sensitivity of a QCM biosensor dramatically improves by thinning the quartz resonator, which also makes the resonance frequency higher, causing high-speed surface movement. Contaminants weakly (nonspecifically) interact with the quartz surface, and they fail to follow the fast surface movement and cannot be detected as the loaded mass. The targets are, however, 1 tightly captured by the receptor proteins immobilized on the surface, and they can move with the surface, contributing to the loaded mass and decreasing the resonant frequency. We develop a MEMS QCM biosensor, in which an AT-cut quartz resonator of 26 µm thick is packaged without fixing, and demonstrate this phenomenon by comparing the frequency changes of fundamental (∼64 MHz) and ninth (∼576 MHz) modes.At ultrahigh-frequency operation with the ninth mode, the sensor response is independent of the amount of impurity proteins, and the binding affinity is unchanged. We then applied this method for the label-free and sandwich-free direct detection of C-reactive protein (CRP) in serum, and confirmed its applicability.

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Reusable gold nanoparticle enhanced QCM immunosensor for detecting C-reactive protein

Cited by 32 publications

References 29 publications

Sensors and Biosensors for C-Reactive Protein, Temperature and pH, and Their Applications for Monitoring Wound Healing: A Review

Sensors and Biosensors for C-Reactive Protein, Temperature and pH, and Their Applications for Monitoring Wound Healing: A Review

Oriented Antibody Covalent Immobilization for Label-Free Impedimetric Detection of C-Reactive Protein via Direct and Sandwich Immunoassays

Ultrahigh-Frequency, Wireless MEMS QCM Biosensor for Direct, Label-Free Detection of Biomarkers in a Large Amount of Contaminants

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