Reusable bio-functionalized surfaces based on electrochemical desorption of benzenediazonium-grafted organic layers

Haque, Al‐Monsur Jiaul; Kim, Kyu‐Won

doi:10.1039/c1cc11866h

Cited by 9 publications

(7 citation statements)

References 18 publications

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“…The first cycle shows a broad irreversible peak around E = -30 mV vs Ag/AgCl, which is assigned to the reduction of the 4-NBD cations and the formation of the corresponding aryl radicals. 23,25 The second cycle is featureless, which suggests blocking of the surface by the products formed during the first cycle, on carbon 23,25 and noncarbon 13,[26][27] surfaces alike. Analogous behavior is observed for 4-NBD modification of graphene, Figure 2c.…”

Section: Resultsmentioning

confidence: 99%

Covalent Modification of Graphene and Graphite Using Diazonium Chemistry: Tunable Grafting and Nanomanipulation

et al. 2015

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We shine light on the covalent modification of graphite and graphene substrates using diazonium chemistry under ambient conditions. We report on the nature of the chemical modification of these graphitic substrates, the relation between molecular structure and film morphology, and the impact of the covalent modification on the properties of the substrates, as revealed by local microscopy and spectroscopy techniques and electrochemistry. By careful selection of the reagents and optimizing reaction conditions, a high density of covalently grafted molecules is obtained, a result that is demonstrated in an unprecedented way by scanning tunneling microscopy (STM) under ambient conditions. With nanomanipulation, i.e., nanoshaving using STM, surface structuring and functionalization at the nanoscale is achieved. This manipulation leads to the removal of the covalently anchored molecules, regenerating pristine sp(2) hybridized graphene or graphite patches, as proven by space-resolved Raman microscopy and molecular self-assembly studies.

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Section: Resultsmentioning

confidence: 99%

Covalent Modification of Graphene and Graphite Using Diazonium Chemistry: Tunable Grafting and Nanomanipulation

et al. 2015

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“…Diazonium-grafted surfaces have found widespread application in different areas such as sensors (Corgier et al 2005b(Corgier et al , 2007 and catalysis (Bourdillon et al 1992b). Diazonium grafted layers have a long-term stability under atmospheric conditions (Allongue et al 1997) and are minimally affected by ultrasound treatments (Adenier et al 2006), high temperatures (Civit et al 2010) and electric potentials (Haque and Kim 2011;Piper et al 2011;Revenga-Parra et al 2012). Diazonium molecules modified with various functional groups have been introduced onto electrodes for immobilisation of biomolecules such as enzymes (Bourdillon et al 1992a;Liu et al 2007;Polsky et al 2007;Radi et al 2006), proteins (Corgier et al 2005a) and antibodies (Corgier et al 2005a;Ho et al 2010) for biosensing application.…”

Section: Stable and Controllable Immobilisation Of Biorecognition Elementioning

confidence: 99%

Diazonium-based impedimetric aptasensor for the rapid label-free detection of Salmonella typhimurium in food sample

Bagheryan

Raoof

Golabi

et al. 2016

Biosensors and Bioelectronics

137

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Fast and accurate detection of microorganisms is of key importance in clinical analysis and in food and water quality monitoring. Salmonella typhimurium is responsible for about a third of all cases of foodborne diseases and consequently, its fast detection is of great importance for ensuring the safety of foodstuffs. We report the development of a label-free impedimetric aptamer-based biosensor for S. typhimurium detection. The aptamer biosensor was fabricated by grafting a diazonium-supporting layer onto screen-printed carbon electrodes (SPEs), via electrochemical or chemical approaches, followed by chemical immobilisation of aminated-aptamer. FTIR-ATR, contact angle and electrochemical measurements were used to monitor the fabrication process. Results showed that electrochemical immobilisation of the diazonium-grafting layer allowed the formation of a denser aptamer layer, which resulted in higher sensitivity. The developed aptamer-biosensor responded linearly, on a logarithm scale, over the concentration range 1 × 10(1) to 1 × 10(8)CFU mL(-1), with a limit of quantification (LOQ) of 1 × 10(1) CFU mL(-1) and a limit of detection (LOD) of 6 CFU mL(-1). Selectivity studies showed that the aptamer biosensor could discriminate S. typhimurium from 6 other model bacteria strains. Finally, recovery studies demonstrated its suitability for the detection of S. typhimurium in spiked (1 × 10(2), 1 × 10(4) and 1 × 10(6) CFU mL(-1)) apple juice samples.

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“…[4] Furthermore, grafted layers have been exposed to ultrasound in different organic solvents with no effect on the formed film. [4,9] Their stability upon exposure to elevated temperatures [20] or positive [21,22] and negative [13,16] electric potentials has also been demonstrated, as well as their long-term stability under atmospheric conditions. [4] The modification of substrates with diazonium salts has been carried out in acidic aqueous solution [6,23] or organic [23,24] media and by electrochemical [6,7,20] or spontaneous grafting by immersing the substrate in diazonium salt solution [8,9,11] or by microcontact printing.…”

Section: Introductionmentioning

confidence: 99%

Controlled Zn‐Mediated Grafting of Thin Layers of Bipodal Diazonium Salt on Gold and Carbon Substrates

Torréns

Ortiz

Turner

et al. 2014

Chemistry A European J

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A controlled, rapid, and potentiostat-free method has been developed for grafting the diazonium salt (3,5-bis(4-diazophenoxy)benzoic acid tetrafluoroborate (DCOOH)) on gold and carbon substrates, based on a Zn-mediated chemical dediazonation. The highly stable thin layer organic platforms obtained were characterized by cyclic voltammetry, AFM, impedance, XP, and Raman spectroscopies. A dediazonation mechanism based on radical formation is proposed. Finally, DCOOH was proved as a linker to an aminated electroactive probe.

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Reusable bio-functionalized surfaces based on electrochemical desorption of benzenediazonium-grafted organic layers

Abstract: We describe an electrochemical oxidative desorption of benzenediazonium-grafted organic layers and immobilized proteins on the layers from indium-tin-oxide electrode surfaces.

Cited by 9 publications

References 18 publications

Covalent Modification of Graphene and Graphite Using Diazonium Chemistry: Tunable Grafting and Nanomanipulation

Covalent Modification of Graphene and Graphite Using Diazonium Chemistry: Tunable Grafting and Nanomanipulation

Diazonium-based impedimetric aptasensor for the rapid label-free detection of Salmonella typhimurium in food sample

Controlled Zn‐Mediated Grafting of Thin Layers of Bipodal Diazonium Salt on Gold and Carbon Substrates

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