Diazonium-based impedimetric aptasensor for the rapid label-free detection of Salmonella typhimurium in food sample

Bagheryan, Zahra; Raoof, Jahan Bakhsh; Golabi, Mohsen; Turner, Anthony; Beni, Valerio

doi:10.1016/j.bios.2016.02.024

Cited by 137 publications

(68 citation statements)

References 62 publications

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“…26 Aptamer 45 was chosen by Bagheryan et al to develop a label-free impedimetric aptamer-based biosensor for S. Typhimurium detection. 27 Han et al identified an RNase-resistant RNA aptamer I-2 specifically targeting outer-membrane protein C (OmpC) of S. Typhimurium. This selected aptamer specifically binds to S. Typhimurium but not to gram-positive bacteria ( S. aureus ) or to other gram-negative bacteria ( E. coli O157:H7).…”

Section: α-Toxinmentioning

confidence: 99%

Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

Pan

Liu

Zhang

2018

Journal of Infection

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The entire human population is at risk of infectious diseases worldwide. Thus far, the diagnosis and treatment of human infectious diseases at the molecular and nanoscale levels have been extremely challenging tasks because of the lack of effective probes to identify and recognize biomarkers of pathogens. Oligonucleotide aptamers are a class of small nucleic acid ligands that are composed of single-stranded DNA (ssDNA) or RNA and act as affinity probes or molecular recognition elements for a variety of targets. These aptamers have an exciting potential for diagnose and/or treatment of specific diseases. In this review, we highlight areas where aptamers have been developed as diagnostic and therapeutic agents for both bacterial and viral infectious diseases as well as aptamer-based detection.

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Section: α-Toxinmentioning

confidence: 99%

Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

Pan

Liu

Zhang

2018

Journal of Infection

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“…A label-free impedimetric aptamer-based biosensor for S. typhimurium was also fabricated by grafting a diazonium-supporting layer onto SPCEs followed by immobilization of an aminated-aptamer. This strategy allowed obtaining a dense aptamer layer, which resulted in a high sensitivity with a LOD of 8 cfu·mL −1 [123]. Also, a specific surface antigen, OmpD, was recently reported as a surface biomarker for detecting S. typhimurium.…”

Section: Electrochemical Affinity Biosensors For Microorganismsmentioning

confidence: 99%

“…), which is of key importance in food and water quality monitoring. Table 4 summarizes relevant information regarding recent reports on electrochemical affinity biosensors for microorganisms [118][119][120][121][122][123][124][125][126] and some important features of selected configurations are commented below.…”

Section: Electrochemical Affinity Biosensors For Microorganismsmentioning

confidence: 99%

Electrochemical Affinity Biosensors in Food Safety

2017

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Safety and quality are key issues of today's food industry. Since the food chain is becoming more and more complex, powerful analytical methods are required to verify the performance of food safety and quality systems. Indeed, such methods require high sensitivity, selectivity, ability for rapid implementation and capability of automatic screening. Electroanalytical chemistry has, for decades, played a relevant role in food safety and quality assessment, taking more and more significance over time in the solution of analytical problems. At present, the implementation of electrochemical methods in the food is evident. This is in a large part due to the relevant results obtained by combining the attractive advantages of electrochemical transduction strategies (in terms of relatively simple hardware, versatility, interface with automatic logging and feasibility of application outside the laboratory environment) with those from biosensors technology. Important examples of enzyme electrochemical biosensors are those dedicated to the determination of glucose, alcohol or cholesterol are important examples. In addition, other types of different electrochemical biosensing approaches have emerged strongly in the last years. Among these, the strategies involving affinity interactions have been shown to possess a large number of applications. Therefore, electrochemical immunosensors and DNA-based biosensors have been widely used to determine major and minor components in foodstuffs, providing sufficient data to evaluate food freshness, the quality of raw materials, or the origin of samples, as well as to determine a variety of compounds at trace levels related to food safety such as micotoxins, allergens, drugs residues or pathogen microorganisms. This review discusses some critical examples of the latest advances in this area, pointing out relevant methodologies related to the measurement techniques, including the use of nanostructured electrodes and strategies for signal amplification.

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“…Electrospun detection technique combined magnetic separation technique, capillary immunoassay, and direct electrical measurement for rapid and accurate sensing of the E. coli O157:H7 cells, the electrospun biosensor device has a linear detection for E. coli O157:H7 concentration of 10 1 to 10 4 CFU/mL with a low concentration of 67 CFU/mL . The impedimetric biosensor approach was implemented and studied (See Table ) by measuring the change in the impedance of the electrodes which indicates the presence or absence of the pathogen cells. These pathogen cells bind to the receptors such as antibodies that are immobilized on the interdigitated electrode arrays (IDEAs).…”

Section: Introductionmentioning

confidence: 99%

Microfluidic based impedance biosensor for pathogens detection in food products

et al. 2019

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A MEMS‐based impedance biosensor was designed, fabricated, and tested to effectively detect the presence of bacterial cells including E. coli O157:H7 and Salmonella typhimurium in raw chicken products using detection region made of multiple interdigitated electrode arrays. A positive dielectrophoresis based focusing electrode was used in order to focus and concentrate the bacterial cells at the centerline of the fluidic microchannel and direct them toward the detection microchannel. The biosensor was fabricated using surface micromachining technology on a glass substrate. The results demonstrate that the device can detect Salmonella with concentrations as low as 10 cells/mL in less than 1 h. The device sensitivity was improved by the addition of the focusing electrodes, which increased the signal response by a factor between 6 and 18 times higher than without the use of the focusing electrodes. The biosensor is selective and can detect other types of pathogen by changing the type of the antibody immobilized on the detection electrodes. The device was able to differentiate live from dead bacteria.

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Diazonium-based impedimetric aptasensor for the rapid label-free detection of Salmonella typhimurium in food sample

Cited by 137 publications

References 62 publications

Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

Electrochemical Affinity Biosensors in Food Safety

Microfluidic based impedance biosensor for pathogens detection in food products

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