Retrospective study of pathology-based investigative techniques for the assessment of diet-induced changes in liver and intestine of flatfish

Abstract

(2018) Retrospective study of pathology-based investigative techniques for the assessment of diet-induced changes in liver and intestine of flatfish,

Effects of Dietary Plant Protein Replacement with Insect and Poultry By-Product Meals on the Liver Health and Serum Metabolites of Sea Bream (Sparus aurata) and Sea Bass (Dicentrarchus labrax)

“…Histological investigations represent an initial step in detecting potential changes associated with inflammation, degeneration, and cell death. These investigations are crucial for assessing the effects of novel diet formulations in feeding trials on liver metabolism and health [48,49,79].…”

“…Hepatocyte and nucleus area were measured (90 cells per fish) on microphotographs using the Image J 1.41 software [48], and the nucleus/hepatocyte (N/H) ratio was calculated. The degree of lipid accumulation (seen as cytoplasmatic vacuoles) and the position of nucleus were recorded, for each measured hepatocyte, on the same microphotograph, for the classification of each liver sample into the mild, moderate, or severe lipid accumulation classes, and subsequently, for the comparison of measurements as a function of dietary treatment.…”

“…Due to its role in metabolizing products from the digestive tract, the liver is also considered a valuable marker of nutritional pathologies [47]. Therefore, histopathology, which provides an analytical view of diseases and their impacts on tissues and cells, represents a valuable tool in studies on fish nutrition, particularly in feeding trials aimed at evaluating the suitability of alternative ingredients for innovative aquafeed [48,49]. Histological qualitative and semi-quantitative methods for evaluating hepatic tissue alterations have been widely applied in farmed fish to assess the effects of different diets on liver health.…”

Chitosan and chitooligosaccharides attenuate soyabean meal-induced intestinal inflammation of turbot (Scophthalmus maximus): possible involvement of NF-кB, activator protein-1 and mitogen-activated protein kinases pathways

“…This enhanced growth performance appears to translate into better intestinal health status, in response to CTS and COS supplementation. SBM-mediated inflammation of fish DI is characterised by swelling of the subepithelial mucosa and lamina propria, as well as by strong infiltration of various pro-inflammatory cells and overexpression of inflammation-related cytokines (51,52) . In turbot, the infiltration of inflammatory cells and expression of a subset of inflammation-related cytokines were promoted by SBM in a dose-dependent fashion, as indicated previously (3) .…”

Histochemical study of the intestine in the common carp Cyprinus carpio fingerlings to detect the impact of the replacement of dietary fish and soybean meals with shrimp wastes

“…Histochemical examinations of digestive system have been widely studied in several teleost fish species (Fiertak & Kilarski, 2002;Domeneghini et al, 2005). Moreover, histochemical studies are valid appliance for determining the type of cell content, also permit to detect cell damage even in mild signs (Sirri et al, 2017). The current study was carried out to investigate the effect of replacement fish meal and soybean meal with shrimp waste meal on intestine in common carp Cyprinus carpio fingerlings using different levels of replacement .This study applied new methodology for discovering the intestinal defects caused by diets replacement using histochemical stains.…”