Retraction Statement: Inhibition of <i>miR‐499‐5p</i> expression improves nonalcoholic fatty liver disease

Liu, Hanyun; Wang, Ting; Chen, Xi; Jiang, Jing; Song, Nianhua; Ran, Li; Xin, Yongning; Xuan, Shi‐Ying

doi:10.1111/ahg.12374

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…Furthermore, miR-499 genetic variation has also been reported as a crucial indicator of diabetic neuropathy susceptibility and could indicate patients with a higher risk of developing cardiovascular autonomic neuropathy [76]. Recently, a link between miR-499 and NAFLD was also discovered, as it was demonstrated by Hanyun et al [77] that miR-499 inhibition improves NAFLD which, as was previously mentioned, is often associated with insulin resistance. The experimental model involved specific pathogen-free (SPF) male C57BL/6 mice fed with a high-fat diet and injected with an miR-499 inhibitor.…”

Section: Mir 499-5pmentioning

confidence: 84%

Non-Coding RNAs as Potential Novel Biomarkers for Early Diagnosis of Hepatic Insulin Resistance

Pielok

Marycz

2020

IJMS

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In the recent years, the prevalence of metabolic conditions such as type 2 Diabetes (T2D) and metabolic syndrome (MetS) raises. The impairment of liver metabolism resulting in hepatic insulin resistance is a common symptom and a critical step in the development of T2D and MetS. The liver plays a crucial role in maintaining glucose homeostasis. Hepatic insulin resistance can often be identified before other symptoms arrive; therefore, establishing methods for its early diagnosis would allow for the implementation of proper treatment in patients before the disease develops. Non-coding RNAs such as miRNAs (micro-RNA) and lncRNAs (long-non-coding RNA) are being recognized as promising novel biomarkers and therapeutic targets—especially due to their regulatory function. The dysregulation of miRNA and lncRNA activity has been reported in the livers of insulin-resistant patients. Many of those transcripts are involved in the regulation of the hepatic insulin signaling cascade. Furthermore, for several miRNAs (miR-802, miR-499-5p, and miR-122) and lncRNAs (H19 imprinted maternally expressed transcript (H19), maternally expressed gene 3 (MEG3), and metastasis associated lung adenocarcinoma transcript 1 (MALAT1)), circulating levels were altered in patients with prediabetes, T2D, and MetS. In the course of this review, the role of the aforementioned ncRNAs in hepatic insulin signaling cascade, as well as their potential application in diagnostics, is discussed. Overall, circulating ncRNAs are precise indicators of hepatic insulin resistance in the development of metabolic diseases and could be applied as early diagnostic and/or therapeutic tools in conditions associated with insulin resistance.

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Section: Mir 499-5pmentioning

confidence: 84%

Non-Coding RNAs as Potential Novel Biomarkers for Early Diagnosis of Hepatic Insulin Resistance

Pielok

Marycz

2020

IJMS

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“…miR-133a could inhibit the browning of adipocytes [ 19 ], which may partly decrease adipocyte loss. Inhibition of miR-499-5p expression in mice with nonalcoholic fatty liver could reduce the accumulation of lipid and triglyceride and reduce total cholesterol in serum in individual mice [ 20 ]. miR-133a-5p was down-regulated in subcutaneous fat deposition in rats fed high-fat diet (HFD), but up-regulated in HFD mice [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

Screening of key miRNAs related with the differentiation of subcutaneous adipocytes and the validation of miR-133a-3p functional significance in goats

Zhang

Wang

et al. 2023

Anim Biosci

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Screening of key miRNAs related with the differentiation of subcutaneous adipocytes and the validation of miR-133a-3p functional significance in goats ABSTRACTObjective: Adipocyte differentiation is regulated by a variety of functional genes and non-coding RNAs. However, the role of miRNAs in lipid deposition of goat white adipose tissue is still unclear.Therefore, this study revealed the miRNA expression profile in goat subcutaneous adipocytes by sRNA-seq. Methods:The miRNA expressed in goat subcutaneous preadipocytes and the mature adipocytes were sequenced by sRNA-seq. The differentially expressed miRNAs (DEm) were screened, and GO and KEGG analyses were performed. Gain-of-function and loss-of-function combined with Oil Red O staining, Bodipy staining and qPCR were utilized to determine the effect of miR-133a-3p on adipocyte differentiation.Results: A total of 218 DEm were screened out. The target genes of these DEm were significantly enriched in GO items such as biological regulation and in KEGG terms such as FAK signaling pathway and MAPK signaling pathway. qPCR verified that the expression trend of miRNA was consistent with miRNA-seq. The gain-of-function or loss-of-function of miR-133a-3p showed that it promoted or inhibited the accumulation of lipid droplets, and C/EBPα and C/EBPβ were extremely significantly up-regulated or down-regulated respectively (P<0.01), the loss-of-function also led to a significant down-regulation of PPARγ (P<0.01). Conclusion:This study successfully identified miRNAs expression patterns in goat subcutaneous adipocytes, and functional identification indicates that miR-133a-3p is a positive regulator of the differentiation process of goat subcutaneous adipocytes. Our results lay the foundation for the molecular mechanism of lipid deposition in meat-source goats from the perspective of miRNA.

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“…Although recent studies have reported that miRNAs are involved in the development of intramuscular preadipocytes [ 57 , 58 ], the molecular regulation mechanism of miRNAs in porcine IMF development remains largely unknown. Studies have shown that inhibition of ssc-miR-499-5p expression in nonalcoholic fatty liver disease ( NAFLD ) cells reduces lipid deposition and of triglyceride (TG) content [ 59 ]. However, in this study, ssc-miR-499-5p was upregulated in high-IMF animals, which may be due to the high conservation and tissue specificity of miRNA, which plays different roles in different tissues.…”

Section: Discussionmentioning

confidence: 99%

Systematic Identification and Comparison of the Expressed Profiles of lncRNAs, miRNAs, circRNAs, and mRNAs with Associated Co-Expression Networks in Pigs with Low and High Intramuscular Fat

Cheng

Liang

Yang

et al. 2021

Animals

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Intramuscular fat (IMF) content is a complex trait that affects meat quality and determines pork quality. In order to explore the potential mechanisms that affect the intramuscular fat content of pigs, a Large white × Min pigs F2 resource populations were constructed, then whole-transcriptome profile analysis was carried out for five low-IMF and five high-IMF F2 individuals. In total, 218 messenger RNA (mRNAs), 213 long non-coding RNAs (lncRNAs), 18 microRNAs (miRNAs), and 59 circular RNAs (circRNAs) were found to be differentially expressed in the longissimus dorsi muscle. Gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes annotations revealed that these differentially expressed (DE) genes or potential target genes (PTGs) of DE regulatory RNAs (lncRNAs, miRNAs, and circRNAs) are mainly involved in cell differentiation, fatty acid synthesis, system development, muscle fiber development, and regulating lipid metabolism. In total, 274 PTGs were found to be differentially expressed between low- and high-IMF pigs, which indicated that some DE regulatory RNAs may contribute to the deposition/metabolism of IMF by regulating their PTGs. In addition, we analyzed the quantitative trait loci (QTLs) of DE RNAs co-located in high- and low-IMF groups. A total of 97 DE regulatory RNAs could be found located in the QTLs related to IMF. Co-expression networks among different types of RNA and competing endogenous RNA (ceRNA) regulatory networks were also constructed, and some genes involved in type I diabetes mellitus were found to play an important role in the complex molecular process of intramuscular fat deposition. This study identified and analyzed some differential RNAs, regulatory RNAs, and PTGs related to IMF, and provided new insights into the study of IMF formation at the level of the genome-wide landscape.

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Retraction Statement: Inhibition of miR‐499‐5p expression improves nonalcoholic fatty liver disease

Cited by 7 publications

References 35 publications

Non-Coding RNAs as Potential Novel Biomarkers for Early Diagnosis of Hepatic Insulin Resistance

Non-Coding RNAs as Potential Novel Biomarkers for Early Diagnosis of Hepatic Insulin Resistance

Screening of key miRNAs related with the differentiation of subcutaneous adipocytes and the validation of miR-133a-3p functional significance in goats

Systematic Identification and Comparison of the Expressed Profiles of lncRNAs, miRNAs, circRNAs, and mRNAs with Associated Co-Expression Networks in Pigs with Low and High Intramuscular Fat

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