Retracted: Urinary circulating DNA and circulating antigen for diagnosis of schistosomiasis mansoni: a field study

Abstract

Objective To evaluate three non‐invasive assays for the diagnosis of schistosomiasis mansoni in an Egyptian village. Methods Urine was collected for the detection of circulating cathodic antigen (CCA) and cell‐free parasite DNA (cfpd) by Point‐of‐contact (POC)‐cassette assay and PCR, respectively. These tests were compared to Kato‐Katz (KK) faecal thick smear for detection of Schistosoma mansoni eggs. Results Disease prevalence by POC‐CCA assay was 86%; by PCR it was 39% vs. 27% by KK. Compared to KK, the sensitivity of POC‐CCA reached 100%, but its specificity was only 19.2% with 41% accuracy. Sensitivity of the PCR assay for cfpd was 55.56%, and specificity was 67.12% with 64% accuracy. A new end point was calculated for combined analysis of KK, POC‐CCA assay and PCR. Sensitivity for the three tests was 52.94%, 90.2% and 76.47%; specificity was 100% for KK and PCR and 18.37% for POC‐CCA. The accuracy calculated for the three tests at the end point was 76% for KK, 55% for POC‐CCA assay and 88% for PCR. Conclusion Conventional PCR assay for detection of cfpd provides a potential screening tool for intestinal schistosomiasis with reliable specificity, reasonable accuracy and affordable financial and technical cost.

Accuracy of Two Circulating Antigen Tests for the Diagnosis and Surveillance of Schistosoma mansoni Infection in Low-Endemicity Settings of Côte d’Ivoire

“… 14 , 35 A recent study conducted in a village in Kafr El Dewar province in Egypt revealed that the sensitivity of POC-CCA was significantly higher than that of a PCR technique for S. mansoni diagnosis. 36 The main strengths of the POC-CCA urine cassette test are the relative low cost, simplicity of use, and the short duration until results are available compared with UCP-LF CAA test, which still requires a basic laboratory setup. The POC-CCA has been recommended for mapping schistosomiasis mansoni at large scale.…”

Foresight in the diagnosis of trematodiasis: innovations versus routine methods

“…Довольно много исследований проводится с целью поиска новых мишеней для диагностики и дифференцировки различных инвазий, поисков новых антигенов и их комбинации [10,15,18,24,33,65,[77][78][79][80][81][82][83][84]. В качестве новых маркеров исследуют: сапозин-подобный белок [37], микроРНК, выделенные из экстравезикул сыворотки [85,86], катепсин L3 при шистосомозе [87], очищенный антиген паразита молекулярной массой 27 при фасцилезе [14], межгенный спейсерный участок ДНК F. hepatica [17], антиген шистосомул при инвазии S. mansoni [88].…”