RETRACTED: Protective effect of DMDD, isolated from the root of Averrhoa carambola L., on high glucose induced EMT in HK-2 cells by inhibiting the TLR4-BAMBI-Smad2/3 signaling pathway

Zhang, Hongliang; Wei, Xiaojie; Lu, Sisi; Lin, Xiaobo; Huang, Jianchun; Chen, Lixiu; Huang, Xiang; Jiang, Liping; Li, Yuchun; Qin, Luhui; Wei, Jinbin; Huang, Renbin

doi:10.1016/j.biopha.2019.108705

Cited by 13 publications

(11 citation statements)

References 33 publications

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“…Wei et al (2018) reported that BK affected TGF-β1-induced epithelial-mesenchymal transition by upregulating the expression of Smad7 and downregulating that of pSmad3 in the TGF-β/Smad pathway, probably as an effective therapy for proliferative vitreoretinopathy. Furthermore, Zhang et al (2019) demonstrated that suppressing the expression of Smad2 remarkably promoted high glucoseinduced fibrosis of human renal tubular epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

Effects of bradykinin on proliferation, apoptosis, and cycle of glomerular mesangial cells via the TGF-β1/Smad signaling pathway

Ding

Wang

et al. 2021

Turk J Biol

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We aimed to assess the effects of bradykinin (BK) on the proliferation, apoptosis, and cycle of glomerular mesangial cells via the transforming growth factor-β 1 (TGF-β1)/Smad signaling pathway. Rat glomerular mesangial cells, HBZY-1, were divided into normal group (untreated), model group (5 ng/L TGF-β1), BK group (5 ng/L TGF-β1 + 1 ng/L BK), and inhibitor group [5 ng/L TGF-β1 + 1 ng/L LY2109761 (TGF-β1-specific inhibitor)]. The cell proliferation, cycle, apoptosis, expression of type I collagen (Col-1), and protein expressions of Col-1, TGF-β1, and phosphorylated Smad2 (p-Smad2) were detected by EdU labeling, flow cytometry, acridine orange/ethidium bromide (AO/EB) dual staining, immunofluorescence assay, and Western blotting, respectively. Compared with the normal group, the cell proliferation rate (P = 0.02) and protein expression levels of Col-1 (P = 0.02), TGF-β1 (P = 0.01), p-Smad2 (P = 0.02), and p-Smad7 (P = 0.00) in the model group significantly increased, and apoptosis rate (P = 0.01) significantly decreased. Compared with the model group, the BK and inhibitor groups significantly decreased in proliferation rate (P = 0.01) and protein expression levels of Col-1 (P = 0.01), TGF-β1 (P = 0.01), and p-Smad2 (P = 0.00). Also, they were significantly elevated in apoptosis rate (P = 0.02) and p-Smad7 protein expression (P = 0.02). BK regulates the proliferation, apoptosis, and the cycle of glomerular mesangial cells by inhibiting the TGF-β1/Smad signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Effects of bradykinin on proliferation, apoptosis, and cycle of glomerular mesangial cells via the TGF-β1/Smad signaling pathway

Ding

Wang

et al. 2021

Turk J Biol

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“…12 Our previous works have revealed that the extract of Averrhoa carambola L. root had a high hypoglycemic activity on streptozotocin-induced diabetic mice. 13,14 The present research was projected to increase the hypoglycemic activity of BACR in diabetic animals. Figure 6 Pancreatic histological changes by HE staining (400×) (n=10).…”

Section: Discussionmentioning

confidence: 99%

<p>Protective Effect of Benzoquinone Isolated from the Roots of <em>Averrhoa carambola</em> L. on Streptozotocin-Induced Diabetic Mice by Inhibiting the TLR4/NF-κB Signaling Pathway</p>

Qin¹,

Zhang²,

Zhou³

et al. 2020

DMSO

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Background: Studies have demonstrated that the roots of Averrhoa carambola L. (Oxalidaceae), a traditional Chinese medicine, can be used to treat diabetes and diabetesrelated diseases. Nevertheless, the potential beneficial effects and mechanism of benzoquinone isolated from the roots of Averrhoa carambola L. (BACR) on diabetes remain unclear. Methods: Diabetic Kunming mice were injected with STZ (120 mgkg −1) in the tail vein. Fasting blood glucose (FBG) and the change of body weight were measured after oral administration of BACR (120, 60, 30 mg/kg/d) every week. The levels of the total cholesterol (TC), triglyceride (TG), free fatty acids (FFA), glucosylated hemoglobin (GHb), fasting insulin (FINS), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were measured. The histological examination of pancreatic tissues and the TLR4/NF-κB pathway was analyzed by RT-PCR, immunohistochemistry and Western blot. Results: The study found that clearly the BACR obviously reduced the blood glucose, serum lipids, GHb and FINS. In addition, BACR treatment markedly reduced the release of inflammatory factors, including IL-6 and TNF-α, and down-regulated the expression of the TLR4/NF-κB pathway. Conclusion: BACR has potential benefits for the treatment of diabetes by ameliorating metabolic functions and attenuating the inflammatory response via inhibition of the activation of theTLR4/NF-κB pathway.

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“…After the treatments, 10 μL CCK‐8 solution was added into each well, and the cells were additionally cultured at 37 °C for 3 h to check cell viability. Data were obtained by microplate reader (Infinite M200 pro; Tecan Inc., Männedorf, Switzerland) at 450 nm absorbance ( A 450 nm ) [17].…”

Section: Methodsmentioning

confidence: 99%

Pyruvate alleviates high glucose‐induced endoplasmic reticulum stress and apoptosis in HK‐2 cells

et al. 2020

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Endoplasmic reticulum (ER) stress plays a critical role in the development of diabetic nephropathy (DN). We previously demonstrated that pyruvate (Pyr)‐enriched oral rehydration solution improved glucometabolic disorders and ameliorated DN outcome in db/db mice. Here, we investigated the effects of Pyr on high glucose‐induced ER stress and apoptosis in HK‐2 cells. Our results suggest that high glucose can induce reactive oxygen species production, apoptosis and ER stress in HK‐2 cells, and that Pyr treatment can ameliorate these effects and restore the expression of key proteins involved in ER stress. Thus, Pyr may have potential for the development of novel strategies for the prevention and treatment of clinical DN.

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RETRACTED: Protective effect of DMDD, isolated from the root of Averrhoa carambola L., on high glucose induced EMT in HK-2 cells by inhibiting the TLR4-BAMBI-Smad2/3 signaling pathway

Cited by 13 publications

References 33 publications

Effects of bradykinin on proliferation, apoptosis, and cycle of glomerular mesangial cells via the TGF-β1/Smad signaling pathway

Effects of bradykinin on proliferation, apoptosis, and cycle of glomerular mesangial cells via the TGF-β1/Smad signaling pathway

<p>Protective Effect of Benzoquinone Isolated from the Roots of <em>Averrhoa carambola</em> L. on Streptozotocin-Induced Diabetic Mice by Inhibiting the TLR4/NF-κB Signaling Pathway</p>

Pyruvate alleviates high glucose‐induced endoplasmic reticulum stress and apoptosis in HK‐2 cells

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