RETRACTED: miRNA‐200b improves hepatic fibrosis induced by CCL₄ by regulating toll‐like receptor 4 in mice

Abstract

To study the effect of miRNA-200b on hepatic fibrosis induced by CCl 4 in mice. The C59BL/6 mice were randomly divided into three groups (normal control [NC], CCLR model [Model], and CCl 4 + miRNA-200b [miRNA]). The hepatic fibrosis was induced by CCl 4 injected subcutaneously twice per week in Model and miRNA groups. After 6 weeks building model, the mice of miRNA group were injected the miRNA-200b from caudal vein twice per week. The mice of Model and miRNA groups were continuously fed for 3 weeks. The IL-1β, IL-6, and TNF-α concentrations of serum were measured by enzyme-linked immunosorbent assay. The hepatic tissues of difference groups were observed by hematoxylin and eosin (H&E) staining, sirius red staining, Masson staining, and terminal deoxynucleotidyl transferase dUTP nick end labeling assay and measured toll-like receptor 4 (TLR4) and nuclear factor-κB (NF-κB) proteins expressions by western blot assay. The correlation between miRNA-200b and TLR4 were analyzed by dual luciferase target assay. Compared with NC group, the interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) concentrations of Model group were significantly upregulated (P < 0.05, respectively). With miRNA-200b overexpression, the IL-1β, IL-6, and TNF-α concentrations were significantly suppressed (P < 0.05, respectively). The pathologies were improved by H&E staining, sirius red staining, and Masson staining; meanwhile, the hepatic cell apoptosis rate was significantly suppressed (P < 0.05). The TLR4 and NF-κB protein expressions of miRNA group were significantly suppressed compared with the Model group (P < 0.05, respectively). By dual luciferase target assay, the TLR4 was a target gene of miRNA-200b. The miRNA-200b upregulation improved hepatic fibrosis induced by CCl 4 via regulation of TLR4 in vivo.

Sulforaphane Attenuates Nonalcoholic Fatty Liver Disease by Inhibiting Hepatic Steatosis and Apoptosis

“…An assay for terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL, Roche Diagnostics, Germany) was conducted to detect hepatocyte apoptosis [ 19 ]. The hepatic tissue slices of different groups were conventional dewaxing and rehydration, and apoptosis was measured by TUNEL assay.…”

Endothelial Cell-Derived Extracellular Vesicles Target TLR4 via miRNA-326-3p to Regulate Skin Fibroblasts Senescence

“…TLR4 mRNA expression was weakened by miR-326-3p. There is a strong link between TLR4 and apoptosis in cells [ 34 ]. In the last report, miR-21 overexpression inhibits TLR4/NF- κ B pathway and reduces apoptosis levels and inflammation incidence in rat cardiomyocytes [ 35 ].…”