RETRACTED: <i>LncAPC</i> drives Wnt/β‐catenin activation and liver TIC self‐renewal through EZH2 mediated APC transcriptional inhibition

Fu, Xiaomin; Lin, Jizhen; Qin, Fujun; Yang, Zihe; Ding, Yanqiao; Zhang, Yong; Han, Lu; Zhu, Xiaoyan; Zhang, Qinxian

doi:10.1002/mc.22764

Cited by 24 publications

(17 citation statements)

References 42 publications

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“…Although CDH1 is a well-known repressed gene by EZH2 [29,30], our study suggested, for the first time, that numerous additional genes were also controlled by EZH2 and KDM6B during EMT. Interestingly, it has been previously reported in liver cancers that EZH2 repressed, in an H3K27me3-dependent manner, the APC promoter leading to the activation of the Wnt/β-Catenin pathway [31]. Interestingly, our study confirmed the presence of both EZH2 and the H3K27me3 mark on the APC promoter (data not shown) demonstrating the validity of our model and experimental scheme.…”

Section: Discussionsupporting

confidence: 84%

EZH2 and KDM6B Expressions Are Associated with Specific Epigenetic Signatures during EMT in Non Small Cell Lung Carcinomas

Lachat

Bruyère

Etcheverry

et al. 2020

Cancers

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The role of Epigenetics in Epithelial Mesenchymal Transition (EMT) has recently emerged. Two epigenetic enzymes with paradoxical roles have previously been associated to EMT, EZH2 (Enhancer of Zeste 2 Polycomb Repressive Complex 2 (PRC2) Subunit), a lysine methyltranserase able to add the H3K27me3 mark, and the histone demethylase KDM6B (Lysine Demethylase 6B), which can remove the H3K27me3 mark. Nevertheless, it still remains unclear how these enzymes, with apparent opposite activities, could both promote EMT. In this study, we evaluated the function of these two enzymes using an EMT-inducible model, the lung cancer A549 cell line. ChIP-seq coupled with transcriptomic analysis showed that EZH2 and KDM6B were able to target and modulate the expression of different genes during EMT. Based on this analysis, we described INHBB, WTN5B, and ADAMTS6 as new EMT markers regulated by epigenetic modifications and directly implicated in EMT induction.

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Section: Discussionsupporting

confidence: 84%

EZH2 and KDM6B Expressions Are Associated with Specific Epigenetic Signatures during EMT in Non Small Cell Lung Carcinomas

Lachat

Bruyère

Etcheverry

et al. 2020

Cancers

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“…In our experiment, transcriptome analysis after LIPUS stimulation revealed significant differences in 27 genes, with 15 genes up-regulated and 12 down-regulated. Among them, KMT2A, APC, DYRK1A, and other genes that played an important role in transcriptional activation 45 , 46 , cell adhesion 47 , and regulation of cell proliferation signals, were up-regulated 48 . Meanwhile, WFS1 and other genes that participated in the occurrence and development of a variety of diseases, were down-regulated 49 .…”

Section: Discussionmentioning

confidence: 99%

Impact of low-intensity pulsed ultrasound on transcription and metabolite compositions in proliferation and functionalization of human adipose-derived mesenchymal stromal cells

Huang

Gao

Wang

et al. 2020

Sci Rep

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To investigate the effect of low-intensity pulsed ultrasound (LIPUS) on the proliferation of human adipose-derived mesenchymal stromal cells (hASCs) and uncovered its stimulation mechanism. LIPUS at 30 mW/cm 2 was applied for 5 min/day to promote the proliferation of hASCs. Flow cytometry was used to study the cell surface markers, cell cycle, and apoptosis of hASCs. The proliferation of hASCs was detected by cell counting kit-8, cell cycle assay, and RT-PCR. The expression of hASCs cytokines was determined by ELISA. The differences between transcriptional genes and metabolites were analyzed by transcript analysis and metabolomic profiling experiments. The number of cells increased after LIPUS stimulation, but there was no significant difference in cell surface markers. The results of flow cytometry, RT-PCR, and ELISA after LIPUS was administered showed that the G 1 and S phases of the cell cycle were prolonged. The expression of cell proliferation related genes (CyclinD1 and c-myc) and the paracrine function related gene (SDF-1α) were up-regulated. The expression of cytokines was increased, while the apoptosis rate was decreased. The results of transcriptome experiments showed that there were significant differences in 27 genes;15 genes were up-regulated, while 12 genes were down-regulated. The results of metabolomics experiments showed significant differences in 30 metabolites; 7 metabolites were up-regulated, and 23 metabolites were down-regulated. LIPUS at 30 mW/cm 2 intensity can promote the proliferation of hASCs cells in an undifferentiating state, and the stem-cell property of hASCs was maintained. CyclinD1 gene, c-myc gene, and various genes of transcription and products of metabolism play an essential role in cell proliferation. This study provides an important experimental and theoretical basis for the clinical application of LIPUS in promoting the proliferation of hASCs cells. As research into our understanding of possible applications, stem cell research advances, stem cell therapies have been used to treat multiple diseases: angiogenesis 1 , diabetes 2 , hepatic failure 3 , multiple scleroses 3,4 , spinal cord injury 5 , glaucomas 6 , and Crohn's diseases 7. In treating these diseases, stem cell therapies have achieved promising results.

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“…On the contrary, lncAPC inhibits transcription of APC via recruiting EZH2 to be a APC promoter, which facilitates the activation of Wnt/β-catenin signaling and liver TIC self-renewal [24] ( Figure 1C). Overexpression of lncWDR26 (GenBank Accession no.…”

Section: Transcriptional Regulationmentioning

confidence: 99%

The Emerging Role of Long Non-Coding RNAs in the Metastasis of Hepatocellular Carcinoma

et al. 2019

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Long non-coding RNAs (lncRNAs) play multifaceted roles in modulating gene expression under both physiological and pathological processes. The dysregulation of lncRNAs has been increasingly linked with many human diseases, including a plethora of cancers. Mounting evidence indicates that lncRNAs are aberrantly expressed in hepatocellular carcinoma (HCC) and can regulate HCC progression, as well as metastasis. In this review, we summarize the recent findings on the expanding roles of lncRNAs in modulating various functions of HCC, and elaborate on how can lncRNAs impact HCC metastasis and progression via interacting with chromatin, RNA, and proteins at the epigenetic, transcriptional, and post-transcriptional levels. This mini-review also highlights the current advances regarding the signaling pathways of lncRNAs in HCC metastasis and sheds light on the possible application of lncRNAs for the prevention and treatment of HCC.

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RETRACTED: LncAPC drives Wnt/β‐catenin activation and liver TIC self‐renewal through EZH2 mediated APC transcriptional inhibition

Cited by 24 publications

References 42 publications

EZH2 and KDM6B Expressions Are Associated with Specific Epigenetic Signatures during EMT in Non Small Cell Lung Carcinomas

EZH2 and KDM6B Expressions Are Associated with Specific Epigenetic Signatures during EMT in Non Small Cell Lung Carcinomas

Impact of low-intensity pulsed ultrasound on transcription and metabolite compositions in proliferation and functionalization of human adipose-derived mesenchymal stromal cells

The Emerging Role of Long Non-Coding RNAs in the Metastasis of Hepatocellular Carcinoma

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