Impact of low-intensity pulsed ultrasound on transcription and metabolite compositions in proliferation and functionalization of human adipose-derived mesenchymal stromal cells

Abstract

To investigate the effect of low-intensity pulsed ultrasound (LIPUS) on the proliferation of human adipose-derived mesenchymal stromal cells (hASCs) and uncovered its stimulation mechanism. LIPUS at 30 mW/cm 2 was applied for 5 min/day to promote the proliferation of hASCs. Flow cytometry was used to study the cell surface markers, cell cycle, and apoptosis of hASCs. The proliferation of hASCs was detected by cell counting kit-8, cell cycle assay, and RT-PCR. The expression of hASCs cytokines was determined by ELISA. The differences between transcriptional genes and metabolites were analyzed by transcript analysis and metabolomic profiling experiments. The number of cells increased after LIPUS stimulation, but there was no significant difference in cell surface markers. The results of flow cytometry, RT-PCR, and ELISA after LIPUS was administered showed that the G 1 and S phases of the cell cycle were prolonged. The expression of cell proliferation related genes (CyclinD1 and c-myc) and the paracrine function related gene (SDF-1α) were up-regulated. The expression of cytokines was increased, while the apoptosis rate was decreased. The results of transcriptome experiments showed that there were significant differences in 27 genes;15 genes were up-regulated, while 12 genes were down-regulated. The results of metabolomics experiments showed significant differences in 30 metabolites; 7 metabolites were up-regulated, and 23 metabolites were down-regulated. LIPUS at 30 mW/cm 2 intensity can promote the proliferation of hASCs cells in an undifferentiating state, and the stem-cell property of hASCs was maintained. CyclinD1 gene, c-myc gene, and various genes of transcription and products of metabolism play an essential role in cell proliferation. This study provides an important experimental and theoretical basis for the clinical application of LIPUS in promoting the proliferation of hASCs cells. As research into our understanding of possible applications, stem cell research advances, stem cell therapies have been used to treat multiple diseases: angiogenesis 1 , diabetes 2 , hepatic failure 3 , multiple scleroses 3,4 , spinal cord injury 5 , glaucomas 6 , and Crohn's diseases 7. In treating these diseases, stem cell therapies have achieved promising results.

Impact of low‐intensity pulsed ultrasound on the growth of Schizochytrium sp. for omega‐3 production

“…Previous studies by our group with different algal cells and mammalian cells showed that ultrasounds could enhance cell division as well as affect other metabolic pathways (Huang et al, 2020; Savchenko et al, 2017; Schultz et al, 2017; Xu et al, 2012). The same result was observed for Schizochytrium cells in this study.…”

Effects of low intensity pulsed ultrasound stimulation on metabolic lipolysis of adipocytes

“…We hypothesized that ultrasound stimulation may lead lipolysis. We searched for the ranges of ultrasound intensities to safely simulate adipocytes to induce lipolysis from previous studies [38]. Lipolysis is mainly catalyzed by three major lipases: ATGL, HSL, and MGL, which belong to the standard pathway of adipocyte lipolysis [39].…”

Biophysical and Biomechanical Effect of Low Intensity US Treatments on Pancreatic Adenocarcinoma 3D Cultures

“…While the effect of HIFU is associated with generated heat, the therapeutic effects of LIPUS are traced back to low intensities within the range of 0.07-3 W/cm 2 Spatial Average Temporal Average (SATA) [6] and frequencies of 0.5-5 MHz and a treatment duration of 5-20 min per day [7]. Many studies showed evidence that LIPUS could regulate cell proliferation and differentiation [8]. Induced biomechanical effects, such as radiation force, acoustic streaming, and strain gradients, seem to play a major role in triggering cellular responses at LIPUS exposures.…”