RETRACTED: Exosome-Derived miR-130a Activates Angiogenesis in Gastric Cancer by Targeting C-MYB in Vascular Endothelial Cells

Abstract

Metastasis is a crucial reason for the poor prognosis of gastric cancer. Angiogenesis is closely associated with tumor invasion and metastasis. Cancer-derived exosomes play an important role in the establishment of the tumor microenvironment. In this study, exosomes were isolated by sequential differential centrifugation, and they were verified by transmission electron microscopy. Changes in the biological behavior of human umbilical vein endothelial cells were evaluated with downstream cellular functional experiments. The RNA and protein levels of the miRNA target gene were determined by real-time qPCR and western blotting. A mouse xenograft model was adopted to evaluate the correlation between exosome-derived miR-130a and tumor growth in vivo. We demonstrated that exosomes delivered miR-130a from gastric cancer cells into vascular cells to promote angiogenesis and tumor growth by targeting c-MYB both in vivo and in vitro. miR-130a packaged in exosomes secreted from cancer cells acts as a driver of angiogenesis. Therefore, miR-130a might be a potential biomarker for monitoring the activity of gastric cancer. In addition, suppressing the expression or blocking the transmission of these exosomes might be a novel antiangiogenic therapeutic strategy for gastric cancer.

Exosomes derived from acute myeloid leukemia cells promote chemoresistance by enhancing glycolysis‐mediated vascular remodeling

“…AML cells secrete angiogenic factors involved in vascular remodeling; however, antiangiogenic therapies do not improve AML patient outcome (Duarte et al, 2018), suggesting that AML cells *** *** *** Exosomes play a significant role in the establishment of the tumor microenvironment (X. Zhang, Yuan, et al, 2015) by shuttling oncogenic information that contributes to cancer spread (Yang et al, 2018).…”

Regulation of MYB mediated cisplatin resistance of ovarian cancer cells involves miR-21-wnt signaling axis

“…Moreover, the expression of c-MYB correlated with higher grade ovarian cancer which suggested a direct relationship between c-MYB expression and aggressive ovarian cancer. The oncogenic role of c-MYB is not limited to ovarian cancer and has been reported in hematological malignancies as well as several solid tumors [9][10][11][12][13] , Despite such wealth of information regarding the oncogenic potential of c-MYB, its role in cisplatin resistance was not known prior to this report 9 . The study reported a connection between c-MYB expression and acquired cisplatin resistance, but there is still no clear understanding regarding how c-MYB can influence cisplatin resistance.…”

The cancer exosomes: Clinical implications, applications and challenges

“…Emerging evidence indicates exosomal miRNAs mediate tumor‐associated angiogenesis. Yang et al . performed sequential differential centrifugation and identified that exosomal miR‐130a derived from GC cells could activate vascular cells via binding c‐MYB, indicated that miR‐130a drove angiogenesis (Table ).…”

“…Emerging evidence indicates exosomal miRNAs mediate tumor-associated angiogenesis. Yang et al 47 performed sequential differential centrifugation and identified that exosomal miR-130a derived from GC cells could activate vascular cells via binding c-MYB, indicated that miR-130a drove angiogenesis (Table 1). Zeng et al 48 validated that miR-25-3p, a metastasis-promoting miRNA existing in CRC cells, could be transferred to endothelial cells via exosomes, regulated the expression of VEGFR2 by targeting KLF2 and KLF4, further contributed to increased vascular permeability and angiogenesis (Table 1 and Figs.…”