RETRACTED: Clinical Significance, Cellular Function, and Potential Molecular Pathways of CCT7 in Endometrial Cancer

Abstract

Objective: Endometrial cancer (EC) is a common gynecologic malignancy; myometrial invasion (MI) is a typical approach of EC spreads and an important index to assess tumor metastasis and outcome in EC patients. CCT7 is a member of the TCP1 chaperone family, involved in cytoskeletal protein folding and unfolding. In this study, the role of CCT7 in EC development was investigated. Methods: Clinical data for 87 EC cases and expression of CCT7 were analyzed. CCT7 was knocked out using siRNA-CCT7 in Ishikawa and RL95-2 cells, and their function about proliferation, apoptosis, and invasion was further tested. Bioinformatics methods were used to predict the potential pathways of CCT7 in EC development. Results: The rates of CCT7-positive cells in EC and adjacent normal endometrium tissues had a significant difference (67.8 vs. 51.4%, p = 0.035), and the expression rate increased from low to high pathological stage (39.7% in the I/II stage, 71.4% in the III/IV stage, p = 0.029). A similar change was found in protein level. CCT7 expression differed significantly between the deep MI group (>1/2) and the superficial MI group (≤1/2) (p = 0.039). However, there were no differences with respect to age, pathological type, and histological grade. CCT7 suppression induced a function loss in both Ishikawa and RL95-2 cells. Bioinformatics analysis demonstrated that EC patients with lower-level CCT7 expression had better overall survival (p = 0.0081). Gene ontology enrichment indicated that "RNA binding," "Mitochondrion," "Translation," and "Spliceosome" were most significantly enriched potential pathways. Five hub genes, PSMA5, PSMD14, SNRPB, SNRPG, and TXNL4A, were all significantly upregulated in EC and had a positive correlation with CCT7. Conclusions: CCT7 may be involved in EC development by excessively activating tumor cell function to promote MI or distant/nodal metastasis, which may contribute to the prognosis of EC patients.

Overexpression of chaperonin containing TCP1 subunit 7 has diagnostic and prognostic value for hepatocellular carcinoma

“…Each of these analyses revealed that CCT7 was involved in the spliceosome signaling pathway. Spliceosome signaling contributes significantly to the tumorigenesis and progression of several types of tumors [29][30][31][32], and previous research has suggested that CCT7 promotes the progression of endometrial cancer through this pathway [33]. Interestingly, in our study, the genes enriched in the spliceosome pathway were among those most relevant to the survival of HCC patients in the GEPIA database.…”

Prognostic and Genomic Analysis of Proteasome 20S Subunit Alpha (PSMA) Family Members in Breast Cancer

“…PSMA5 , alternatively known as proteasome subunit alpha type 5, encodes the outer α-rings of the 20S core particle. PSMA5 was significantly upregulated in endometrial cancer, while PSMA6 was significantly upregulated in multiple myeloma patients and pancreatic ductal carcinoma cell models [ 85 , 86 ]. PSMA6 , alternatively known as proteasome subunit alpha type 6, encodes the α1 subunit, which makes up the outer ring of the 20S core particle.…”

Overexpression of chaperonin containing TCP1 subunit 7 has diagnostic and prognostic value for hepatocellular carcinoma.

“…Previous research has suggested that CCT7 promoted the progression of endometrial cancer through Spliceosome signaling pathway [35]. In addition, Spliceosome pathway plays a signi cant role in the tumorigenesis and progression of several tumors [36][37][38][39].…”